> top > docs > PubMed:12677400 > annotations

PubMed:12677400 JSONTXT

Annnotations TAB JSON ListView MergeView

DisGeNET

Id Subject Object Predicate Lexical cue
T0 362-398 gene:7422 denotes vascular endothelial growth factor-A
T1 228-236 disease:C0011847 denotes diabetes
T2 362-398 gene:7422 denotes vascular endothelial growth factor-A
T3 228-236 disease:C0011849 denotes diabetes
T4 400-406 gene:7422 denotes VEGF-A
T5 228-236 disease:C0011847 denotes diabetes
T6 400-406 gene:7422 denotes VEGF-A
T7 228-236 disease:C0011849 denotes diabetes
R1 T0 T1 associated_with vascular endothelial growth factor-A,diabetes
R2 T2 T3 associated_with vascular endothelial growth factor-A,diabetes
R3 T4 T5 associated_with VEGF-A,diabetes
R4 T6 T7 associated_with VEGF-A,diabetes

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12677400-10#24#28#gene7422 1834-1838 gene7422 denotes VEGF
12677400-10#123#131#diseaseC0011847 1933-1941 diseaseC0011847 denotes diabetes
12677400-10#123#131#diseaseC0011849 1933-1941 diseaseC0011849 denotes diabetes
24#28#gene7422123#131#diseaseC0011847 12677400-10#24#28#gene7422 12677400-10#123#131#diseaseC0011847 associated_with VEGF,diabetes
24#28#gene7422123#131#diseaseC0011849 12677400-10#24#28#gene7422 12677400-10#123#131#diseaseC0011849 associated_with VEGF,diabetes

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 188-550 OBJECTIVE denotes We studied the gene therapy efficacy of diabetes-associated wound healing disorder with an adeno-associated virus (AAV) vector expressing the 165-amino acid isoform of human vascular endothelial growth factor-A (VEGF-A) by using an incisional skin-wound model produced on the back of female diabetic C57BL/KsJ db+/db+ mice and their normal littermates ( db+/+m).
T2 560-963 METHODS denotes Animals were randomized to receive intradermally into the wound edges either rAAV-LacZ (a control gene), or rAAV-VEGF165. Animals were killed on different days (7 and 14 days after skin injury) and wounded skin tissues were used for gene marker studies, histological evaluation and immunohistochemistry, and wound breaking strength analysis. Furthermore we studied the VEGF mature protein in the wounds.
T3 973-1782 RESULTS denotes We found that AAV vectors are highly efficient for gene transfer to the mouse skin, displaying an exquisite tropism for the panniculus carnosus by using the beta-galactosidase activity assay. We confirmed the increased expression of the angiogenic factor at day 7 by measuring the wound content of the mature protein. Delivery of VEGF165 to incisional skin wounds of diabetic mice resulted in a remarkable induction of new vessel formation with consequent improvement in the wound healing process. The rAAV-VEGF165 gene improved wound healing in diabetic mice through the stimulation of angiogenesis, reepithelization, synthesis and maturation of extracellular matrix. Moreover the recombinant AAV encoding the human VEGF165 increased the breaking strength of the wound and enhanced the wound content of VEGF.
T4 1810-1942 CONCLUSIONS denotes Our study suggests that VEGF gene transfer might represent a new approach to treat wound healing disorders associated with diabetes.