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PubMed:12676895 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 1671-1675 gene:771 denotes CA12
T1 1619-1627 disease:C0017601 denotes glaucoma
T2 1994-1998 gene:771 denotes CA12
T3 2025-2033 disease:C0017601 denotes glaucoma
R1 T0 T1 associated_with CA12,glaucoma
R2 T2 T3 associated_with CA12,glaucoma

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12676895-0#74#78#gene771 74-78 gene771 denotes CA12
12676895-0#115#119#gene771 115-119 gene771 denotes CA12
12676895-0#121#126#gene771 121-126 gene771 denotes CAXII
12676895-0#131#139#diseaseC0017601 131-139 diseaseC0017601 denotes glaucoma
12676895-4#9#14#gene771 721-726 gene771 denotes CAXII
12676895-4#142#154#diseaseC0857112 854-866 diseaseC0857112 denotes glaucoma eye
74#78#gene771131#139#diseaseC0017601 12676895-0#74#78#gene771 12676895-0#131#139#diseaseC0017601 associated_with CA12,glaucoma
115#119#gene771131#139#diseaseC0017601 12676895-0#115#119#gene771 12676895-0#131#139#diseaseC0017601 associated_with CA12,glaucoma
121#126#gene771131#139#diseaseC0017601 12676895-0#121#126#gene771 12676895-0#131#139#diseaseC0017601 associated_with CAXII,glaucoma
9#14#gene771142#154#diseaseC0857112 12676895-4#9#14#gene771 12676895-4#142#154#diseaseC0857112 associated_with CAXII,glaucoma eye

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 150-702 OBJECTIVE denotes Carbonic anhydrase enzymes (CAs) are universally involved in many fundamental physiological processes, including acid base regulation and fluid formation and movement. In glaucoma patients, CA inhibitors are very effective in lowering intraocular pressure by reducing the rate of aqueous humour secretion mediated by the CAs in the ciliary epithelium. In this work, we investigated the expression and tissue distribution of two recently discovered CA genes CA9 (CAIX) and CA12 (CAXII) in fetal, neonatal, and adult human eyes with and without glaucoma.
T2 712-1063 METHODS denotes CAIX and CAXII expression in 16 normal and 10 glaucomatous eyes, and in cultured non-pigmented ciliary epithelial cells (NPE) from normal and glaucoma eye donors was assessed by immunostaining. In addition, northern blot hybridisation was performed to assess expression of CA4, CA9, and CA12 mRNA in cultured NPE cells from normal and glaucoma donors.
T3 1073-1851 RESULTS denotes CAXII was localised primarily to the NPE with its expression prominent during embryonic eye development but which decreased significantly in adults. CAIX expression in the NPE was very low. The epithelium of cornea and lens occasionally expressed both enzymes at low levels during development and in adult eye, and no expression was detected in the retina. The NPE from glaucoma eyes expressed higher levels of CAXII, but not CAIX, in comparison with normal eyes. This expression pattern was retained in cultured NPE cell lines. NPE cells from a glaucoma patient showed a five-fold increase in the CA12 mRNA level with no detectable expression of CA9 mRNA. Also, no expression of the CA4 gene encoding a GPI anchored plasma membrane protein was detected on these northern blots.
T4 1865-2034 CONCLUSIONS denotes Transmembrane CAIX and CAXII enzymes are expressed in the ciliary cells and, thus, may be involved in aqueous humour production. CA12 may be a targeted gene in glaucoma.