| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-101 |
Sentence |
denotes |
Identification of critical residues of an immunodominant region of Echinococcus granulosus antigen B. |
| T2 |
102-182 |
Sentence |
denotes |
Immune evasion strategies often shape the immunogenicity of parasite components. |
| T3 |
183-407 |
Sentence |
denotes |
We recently found that the N-terminal extension of the major subunit of Echinococcus granulosus antigen B (AgB), the causative agent of hydatid disease, concentrates the immunoreactive B cell epitopes of the native molecule. |
| T4 |
408-561 |
Sentence |
denotes |
The nature of this immunodominance was analyzed using four monoclonal antibodies (mAbs) defining overlapping epitopes in this region of the AgB molecule. |
| T5 |
562-663 |
Sentence |
denotes |
The minimal epitope requirements of these mAbs were determined using phage display peptide libraries. |
| T6 |
664-888 |
Sentence |
denotes |
The consensus sequences isolated with the mAbs, and alanine replacement analysis with synthetic peptides mapped the relevant molecular contacts within a short stretch corresponding to residues 17-24 of the AgB major subunit. |
| T7 |
889-1025 |
Sentence |
denotes |
Substitution of two critical residues within this stretch produced a dramatic loss of antigenicity, as determined by using patient sera. |
| T8 |
1026-1251 |
Sentence |
denotes |
The circular dichroism spectra of the antigen, together with the distribution of the contact residues, suggest that this region adopts an amphipathic alpha-helix structure that clusters the contact residues on its polar side. |
| T9 |
1252-1506 |
Sentence |
denotes |
To provide further insight in the interpretation of the structure activity relationships for this immunoreactive region of E. granulosus AgB, we developed a model for the N-terminal extension of the AgB major subunit, which helps to rationalize our data. |
