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PubMed:12646839 JSONTXT

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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 140-725 BACKGROUND denotes Despite a strong association of schwannomin/merlin gene mutations with vestibular schwannoma formation, the regulatory mechanisms and biologic pathways involved are still largely unknown. The hypothesis of this study is that the genesis and growth characteristics of neurofibromatosis type 2 (NF2)-associated vestibular schwannomas are determined by genetic alterations that vary in gene transcript expression; this transcript expression includes oncogenic gene products that may be identified by construction and sequencing of a cDNA library from NF2-associated vestibular schwannoma.
T2 735-1291 METHODS denotes Approximately 3 mL of fresh tumor was obtained during resection of a 4-cm vestibular schwannoma from a patient with NF2. Poly(A)(+) mRNA was isolated, synthesized into double-stranded cDNA, and unidirectionally inserted into Uni-Zap XR (Stratagene, La Jolla, CA) bacteriophage vectors. Bacteriophage vectors containing cDNA inserts were processed into phagemids according to Uni-Zap XR protocol, and inserted vectors were sequenced and analyzed using BLAST software (National Institutes of Health, Bethesda, MD) with GenBank, EMBL, DDBJ, and PBD databases.
T3 1301-1815 RESULTS denotes The cDNA library contained 2.4 million primary plaques. Inserts averaged 1.8 kilobases (kb) in length, with a range of 0.8 to 3.0 kb. BLAST multidatabase comparison of the sequence data obtained from 50 randomly selected clones yielded identification of 13 sequences representing known human genes and 17 sequences representing cloned sequences with unknown function. Three clones represented sequences not previously described in vestibular schwannomas but strongly implicated in oncogenesis within other tissues.
T4 1829-2031 CONCLUSIONS denotes These data have implications for understanding the molecular mechanisms of vestibular schwannoma tumor biology. Identified genes may provide future diagnostic/prognostic markers and therapeutic targets.

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-127 Sentence denotes Gene discovery using a human vestibular schwannoma cDNA library constructed from a patient with neurofibromatosis type 2 (NF2).
TextSentencer_T2 128-139 Sentence denotes BACKGROUND:
TextSentencer_T3 140-327 Sentence denotes Despite a strong association of schwannomin/merlin gene mutations with vestibular schwannoma formation, the regulatory mechanisms and biologic pathways involved are still largely unknown.
TextSentencer_T4 328-725 Sentence denotes The hypothesis of this study is that the genesis and growth characteristics of neurofibromatosis type 2 (NF2)-associated vestibular schwannomas are determined by genetic alterations that vary in gene transcript expression; this transcript expression includes oncogenic gene products that may be identified by construction and sequencing of a cDNA library from NF2-associated vestibular schwannoma.
TextSentencer_T5 726-734 Sentence denotes METHODS:
TextSentencer_T6 735-855 Sentence denotes Approximately 3 mL of fresh tumor was obtained during resection of a 4-cm vestibular schwannoma from a patient with NF2.
TextSentencer_T7 856-1020 Sentence denotes Poly(A)(+) mRNA was isolated, synthesized into double-stranded cDNA, and unidirectionally inserted into Uni-Zap XR (Stratagene, La Jolla, CA) bacteriophage vectors.
TextSentencer_T8 1021-1291 Sentence denotes Bacteriophage vectors containing cDNA inserts were processed into phagemids according to Uni-Zap XR protocol, and inserted vectors were sequenced and analyzed using BLAST software (National Institutes of Health, Bethesda, MD) with GenBank, EMBL, DDBJ, and PBD databases.
TextSentencer_T9 1292-1300 Sentence denotes RESULTS:
TextSentencer_T10 1301-1356 Sentence denotes The cDNA library contained 2.4 million primary plaques.
TextSentencer_T11 1357-1434 Sentence denotes Inserts averaged 1.8 kilobases (kb) in length, with a range of 0.8 to 3.0 kb.
TextSentencer_T12 1435-1668 Sentence denotes BLAST multidatabase comparison of the sequence data obtained from 50 randomly selected clones yielded identification of 13 sequences representing known human genes and 17 sequences representing cloned sequences with unknown function.
TextSentencer_T13 1669-1815 Sentence denotes Three clones represented sequences not previously described in vestibular schwannomas but strongly implicated in oncogenesis within other tissues.
TextSentencer_T14 1816-1828 Sentence denotes CONCLUSIONS:
TextSentencer_T15 1829-1940 Sentence denotes These data have implications for understanding the molecular mechanisms of vestibular schwannoma tumor biology.
TextSentencer_T16 1941-2031 Sentence denotes Identified genes may provide future diagnostic/prognostic markers and therapeutic targets.
T1 0-127 Sentence denotes Gene discovery using a human vestibular schwannoma cDNA library constructed from a patient with neurofibromatosis type 2 (NF2).
T2 128-139 Sentence denotes BACKGROUND:
T3 140-327 Sentence denotes Despite a strong association of schwannomin/merlin gene mutations with vestibular schwannoma formation, the regulatory mechanisms and biologic pathways involved are still largely unknown.
T4 328-725 Sentence denotes The hypothesis of this study is that the genesis and growth characteristics of neurofibromatosis type 2 (NF2)-associated vestibular schwannomas are determined by genetic alterations that vary in gene transcript expression; this transcript expression includes oncogenic gene products that may be identified by construction and sequencing of a cDNA library from NF2-associated vestibular schwannoma.
T5 726-734 Sentence denotes METHODS:
T6 735-855 Sentence denotes Approximately 3 mL of fresh tumor was obtained during resection of a 4-cm vestibular schwannoma from a patient with NF2.
T7 856-1020 Sentence denotes Poly(A)(+) mRNA was isolated, synthesized into double-stranded cDNA, and unidirectionally inserted into Uni-Zap XR (Stratagene, La Jolla, CA) bacteriophage vectors.
T8 1021-1291 Sentence denotes Bacteriophage vectors containing cDNA inserts were processed into phagemids according to Uni-Zap XR protocol, and inserted vectors were sequenced and analyzed using BLAST software (National Institutes of Health, Bethesda, MD) with GenBank, EMBL, DDBJ, and PBD databases.
T9 1292-1300 Sentence denotes RESULTS:
T10 1301-1356 Sentence denotes The cDNA library contained 2.4 million primary plaques.
T11 1357-1434 Sentence denotes Inserts averaged 1.8 kilobases (kb) in length, with a range of 0.8 to 3.0 kb.
T12 1435-1668 Sentence denotes BLAST multidatabase comparison of the sequence data obtained from 50 randomly selected clones yielded identification of 13 sequences representing known human genes and 17 sequences representing cloned sequences with unknown function.
T13 1669-1815 Sentence denotes Three clones represented sequences not previously described in vestibular schwannomas but strongly implicated in oncogenesis within other tissues.
T14 1816-1828 Sentence denotes CONCLUSIONS:
T15 1829-1940 Sentence denotes These data have implications for understanding the molecular mechanisms of vestibular schwannoma tumor biology.
T16 1941-2031 Sentence denotes Identified genes may provide future diagnostic/prognostic markers and therapeutic targets.

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12646839-1#44#50#gene4771 184-190 gene4771 denotes merlin
12646839-1#71#92#diseaseC0027859 211-232 diseaseC0027859 denotes vestibular schwannoma
44#50#gene477171#92#diseaseC0027859 12646839-1#44#50#gene4771 12646839-1#71#92#diseaseC0027859 associated_with merlin,vestibular schwannoma

PubCasesHPO

Id Subject Object Predicate Lexical cue
TI1 29-50 HP:0009588 denotes vestibular schwannoma
AB1 211-232 HP:0009588 denotes vestibular schwannoma
AB2 703-724 HP:0009588 denotes vestibular schwannoma
AB3 809-830 HP:0009588 denotes vestibular schwannoma
AB4 1904-1925 HP:0009588 denotes vestibular schwannoma

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 1807-1814 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues

PubCasesORDO

Id Subject Object Predicate Lexical cue
TI1 96-120 ORDO:637 denotes neurofibromatosis type 2
TI2 122-125 ORDO:637 denotes NF2
AB1 407-431 ORDO:637 denotes neurofibromatosis type 2
AB2 433-436 ORDO:637 denotes NF2
AB3 688-691 ORDO:637 denotes NF2
AB4 851-854 ORDO:637 denotes NF2

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 1807-1814 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues

DisGeNET

Id Subject Object Predicate Lexical cue
T0 172-183 gene:4771 denotes schwannomin
T1 211-232 disease:C0027859 denotes vestibular schwannoma
T2 184-190 gene:4771 denotes merlin
T3 211-232 disease:C0027859 denotes vestibular schwannoma
R1 T0 T1 associated_with schwannomin,vestibular schwannoma
R2 T2 T3 associated_with merlin,vestibular schwannoma