PubMed:12636916 JSONTXT

{"project":"bionlp-st-pc-2013-training","denotations":[{"id":"T1","span":{"begin":40,"end":46},"obj":"Gene_or_gene_product"},{"id":"T2","span":{"begin":79,"end":86},"obj":"Gene_or_gene_product"},{"id":"T3","span":{"begin":198,"end":205},"obj":"Gene_or_gene_product"},{"id":"T4","span":{"begin":236,"end":239},"obj":"Cellular_component"},{"id":"T5","span":{"begin":331,"end":338},"obj":"Gene_or_gene_product"},{"id":"T6","span":{"begin":360,"end":364},"obj":"Gene_or_gene_product"},{"id":"T7","span":{"begin":368,"end":383},"obj":"Gene_or_gene_product"},{"id":"T8","span":{"begin":389,"end":393},"obj":"Gene_or_gene_product"},{"id":"T9","span":{"begin":397,"end":403},"obj":"Gene_or_gene_product"},{"id":"T10","span":{"begin":448,"end":454},"obj":"Gene_or_gene_product"},{"id":"T11","span":{"begin":455,"end":459},"obj":"Gene_or_gene_product"},{"id":"T12","span":{"begin":460,"end":464},"obj":"Gene_or_gene_product"},{"id":"T13","span":{"begin":479,"end":485},"obj":"Gene_or_gene_product"},{"id":"T14","span":{"begin":550,"end":554},"obj":"Gene_or_gene_product"},{"id":"T15","span":{"begin":558,"end":569},"obj":"Cellular_component"},{"id":"T16","span":{"begin":603,"end":607},"obj":"Complex"},{"id":"T17","span":{"begin":628,"end":636},"obj":"Cellular_component"},{"id":"T18","span":{"begin":657,"end":661},"obj":"Gene_or_gene_product"},{"id":"T19","span":{"begin":693,"end":697},"obj":"Gene_or_gene_product"},{"id":"T20","span":{"begin":702,"end":706},"obj":"Gene_or_gene_product"},{"id":"T21","span":{"begin":724,"end":734},"obj":"Complex"},{"id":"T22","span":{"begin":813,"end":820},"obj":"Gene_or_gene_product"},{"id":"T23","span":{"begin":890,"end":896},"obj":"Gene_or_gene_product"}],"text":"Phosphorylation-dependent regulation of septin dynamics during the cell cycle.\nSeptins are GTPases involved in cytokinesis. In yeast, they form a ring at the cleavage site. Using FRAP, we show that septins are mobile within the ring at bud emergence and telophase and are immobile during S, G2, and M phases. Immobilization of the septins is dependent on both Cla4, a PAK-like kinase, and Gin4, a septin-dependent kinase that can phosphorylate the septin Shs1/Sep7. Induction of septin ring dynamics in telophase is triggered by the translocation of Rts1, a kinetochore-associated regulatory subunit of PP2A phosphatase, to the bud neck and correlates with Rts1-dependent dephosphorylation of Shs1. In rts1-Delta cells, the actomyosin ring contracts properly but cytokinesis fails. Together our results implicate septins in a late step of cytokinesis and indicate that proper regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis."}