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PubMed:12634322 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 71-78 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 71-78 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 118-125 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T4 118-125 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T5 194-205 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin
T6 771-792 https://glytoucan.org/Structures/Glycans/G27025MB denotes N-acetylgalactosamine
T7 919-940 https://glytoucan.org/Structures/Glycans/G27025MB denotes N-acetylgalactosamine
T8 1521-1528 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T9 1521-1528 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 71-78 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T3 118-125 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T5 194-205 Glycan_Motif denotes chondroitin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT
T6 771-792 Glycan_Motif denotes N-acetylgalactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB
T7 919-940 Glycan_Motif denotes N-acetylgalactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB
T8 1521-1528 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-101 Sentence denotes Glycosaminoglycan structures required for strong binding to midkine, a heparin-binding growth factor.
TextSentencer_T2 102-241 Sentence denotes Midkine (MK), a heparin-binding growth factor, binds strongly to oversulfated structures in chondroitin sulfates (CSs) and heparan sulfate.
TextSentencer_T3 242-404 Sentence denotes To elucidate the carbohydrate structure actually involved in the strong binding, dissected brains from 13-day mouse embryos were incubated with [14C]-glucosamine.
TextSentencer_T4 405-634 Sentence denotes The labeled glycosaminoglycans were fractionated by MK-agarose affinity chromatography to a weakly binding fraction, which was eluted by 0.5 M NaCl, and a strongly binding fraction, which was eluted by higher NaCl concentrations.
TextSentencer_T5 635-875 Sentence denotes Among the unsaturated disaccharides released from the strongly binding fraction by chondroitinase ABC, DeltaDi-diSE with 4,6-disulfated N-acetylgalactosamine accounted for 32.3%, whereas its content was lower in the weakly binding fraction.
TextSentencer_T6 876-1020 Sentence denotes Artificial CS-E structure was formed using N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase purified from squid or recombinant human enzyme.
TextSentencer_T7 1021-1224 Sentence denotes Analysis of the products and their interaction with MK revealed that E units without 3-O-sulfation of glucuronic acid are sufficient for strong binding, provided that they are present as a dense cluster.
TextSentencer_T8 1225-1431 Sentence denotes Among the sulfated disaccharides released by heparitinase digestion, the trisulfated one, DeltaDiHS-triS, was the most abundant in the strongly binding fraction and was lower in the weakly binding fraction.
TextSentencer_T9 1432-1593 Sentence denotes Together with results of previous studies, we concluded that the multivalent trisulfated heparin-like unit is another structure involved in strong binding to MK.
T1 0-101 Sentence denotes Glycosaminoglycan structures required for strong binding to midkine, a heparin-binding growth factor.
T2 102-241 Sentence denotes Midkine (MK), a heparin-binding growth factor, binds strongly to oversulfated structures in chondroitin sulfates (CSs) and heparan sulfate.
T3 242-404 Sentence denotes To elucidate the carbohydrate structure actually involved in the strong binding, dissected brains from 13-day mouse embryos were incubated with [14C]-glucosamine.
T4 405-634 Sentence denotes The labeled glycosaminoglycans were fractionated by MK-agarose affinity chromatography to a weakly binding fraction, which was eluted by 0.5 M NaCl, and a strongly binding fraction, which was eluted by higher NaCl concentrations.
T5 635-875 Sentence denotes Among the unsaturated disaccharides released from the strongly binding fraction by chondroitinase ABC, DeltaDi-diSE with 4,6-disulfated N-acetylgalactosamine accounted for 32.3%, whereas its content was lower in the weakly binding fraction.
T6 876-1020 Sentence denotes Artificial CS-E structure was formed using N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase purified from squid or recombinant human enzyme.
T7 1021-1224 Sentence denotes Analysis of the products and their interaction with MK revealed that E units without 3-O-sulfation of glucuronic acid are sufficient for strong binding, provided that they are present as a dense cluster.
T8 1225-1431 Sentence denotes Among the sulfated disaccharides released by heparitinase digestion, the trisulfated one, DeltaDiHS-triS, was the most abundant in the strongly binding fraction and was lower in the weakly binding fraction.
T9 1432-1593 Sentence denotes Together with results of previous studies, we concluded that the multivalent trisulfated heparin-like unit is another structure involved in strong binding to MK.
T1 0-101 Sentence denotes Glycosaminoglycan structures required for strong binding to midkine, a heparin-binding growth factor.
T2 102-241 Sentence denotes Midkine (MK), a heparin-binding growth factor, binds strongly to oversulfated structures in chondroitin sulfates (CSs) and heparan sulfate.
T3 242-404 Sentence denotes To elucidate the carbohydrate structure actually involved in the strong binding, dissected brains from 13-day mouse embryos were incubated with [14C]-glucosamine.
T4 405-634 Sentence denotes The labeled glycosaminoglycans were fractionated by MK-agarose affinity chromatography to a weakly binding fraction, which was eluted by 0.5 M NaCl, and a strongly binding fraction, which was eluted by higher NaCl concentrations.
T5 635-875 Sentence denotes Among the unsaturated disaccharides released from the strongly binding fraction by chondroitinase ABC, DeltaDi-diSE with 4,6-disulfated N-acetylgalactosamine accounted for 32.3%, whereas its content was lower in the weakly binding fraction.
T6 876-1020 Sentence denotes Artificial CS-E structure was formed using N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase purified from squid or recombinant human enzyme.
T7 1021-1224 Sentence denotes Analysis of the products and their interaction with MK revealed that E units without 3-O-sulfation of glucuronic acid are sufficient for strong binding, provided that they are present as a dense cluster.
T8 1225-1431 Sentence denotes Among the sulfated disaccharides released by heparitinase digestion, the trisulfated one, DeltaDiHS-triS, was the most abundant in the strongly binding fraction and was lower in the weakly binding fraction.
T9 1432-1593 Sentence denotes Together with results of previous studies, we concluded that the multivalent trisulfated heparin-like unit is another structure involved in strong binding to MK.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 71-78 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 71-78 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 118-125 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T4 118-125 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T5 194-205 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin
T6 771-792 https://glytoucan.org/Structures/Glycans/G27025MB denotes N-acetylgalactosamine
T7 919-940 https://glytoucan.org/Structures/Glycans/G27025MB denotes N-acetylgalactosamine
T8 1521-1528 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T9 1521-1528 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 194-205 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin
T2 225-240 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 333-339 http://purl.obolibrary.org/obo/MAT_0000098 denotes brains
T2 358-365 http://purl.obolibrary.org/obo/MAT_0000226 denotes embryos

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T20 392-403 FMAID:196792 denotes glucosamine
_T1 0-17 FMAID:63011 denotes Glycosaminoglycan
_T2 0-17 FMAID:167395 denotes Glycosaminoglycan
_T3 71-78 FMAID:82839 denotes heparin
_T4 71-78 FMAID:167420 denotes heparin
_T5 118-125 FMAID:167420 denotes heparin
_T6 118-125 FMAID:82839 denotes heparin
_T7 194-214 FMAID:82837 denotes chondroitin sulfates
_T8 194-214 FMAID:196838 denotes chondroitin sulfates
_T9 225-232 FMAID:165191 denotes heparan
_T10 225-232 FMAID:67110 denotes heparan
_T11 225-240 FMAID:167405 denotes heparan sulfate
_T12 225-240 FMAID:63023 denotes heparan sulfate
_T13 259-271 FMAID:197276 denotes carbohydrate
_T14 259-271 FMAID:82737 denotes carbohydrate
_T15 333-339 FMAID:50801 denotes brains
_T16 333-339 FMAID:146514 denotes brains
_T17 358-365 FMAID:169646 denotes embryos
_T18 358-365 FMAID:69068 denotes embryos
_T19 392-403 FMAID:82797 denotes glucosamine
_T21 417-435 FMAID:167395 denotes glycosaminoglycans
_T22 417-435 FMAID:63011 denotes glycosaminoglycans
_T23 657-670 FMAID:82744 denotes disaccharides
_T24 657-670 FMAID:196733 denotes disaccharides
_T25 771-792 FMAID:82786 denotes N-acetylgalactosamine
_T26 771-792 FMAID:196780 denotes N-acetylgalactosamine
_T27 919-940 FMAID:82786 denotes N-acetylgalactosamine
_T28 919-940 FMAID:196780 denotes N-acetylgalactosamine
_T29 1123-1138 FMAID:82798 denotes glucuronic acid
_T30 1123-1138 FMAID:196793 denotes glucuronic acid
_T31 1244-1257 FMAID:82744 denotes disaccharides
_T32 1244-1257 FMAID:196733 denotes disaccharides
_T33 1521-1528 FMAID:167420 denotes heparin
_T34 1521-1528 FMAID:82839 denotes heparin

uniprot-human

Id Subject Object Predicate Lexical cue
T1 60-67 http://www.uniprot.org/uniprot/P21741 denotes midkine
T2 102-109 http://www.uniprot.org/uniprot/P21741 denotes Midkine
T3 111-113 http://www.uniprot.org/uniprot/P21741 denotes MK
T4 457-459 http://www.uniprot.org/uniprot/P21741 denotes MK
T5 1073-1075 http://www.uniprot.org/uniprot/P21741 denotes MK
T6 1590-1592 http://www.uniprot.org/uniprot/P21741 denotes MK
T7 111-113 http://www.uniprot.org/uniprot/Q03426 denotes MK
T8 457-459 http://www.uniprot.org/uniprot/Q03426 denotes MK
T9 1073-1075 http://www.uniprot.org/uniprot/Q03426 denotes MK
T10 1590-1592 http://www.uniprot.org/uniprot/Q03426 denotes MK
T11 718-732 http://www.uniprot.org/uniprot/P34059 denotes chondroitinase
T12 887-889 http://www.uniprot.org/uniprot/O75390 denotes CS
T13 919-942 http://www.uniprot.org/uniprot/P15848 denotes N-acetylgalactosamine 4

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 60-67 http://www.uniprot.org/uniprot/P12025 denotes midkine
T2 102-109 http://www.uniprot.org/uniprot/P12025 denotes Midkine
T3 111-113 http://www.uniprot.org/uniprot/P12025 denotes MK
T4 457-459 http://www.uniprot.org/uniprot/P12025 denotes MK
T5 1073-1075 http://www.uniprot.org/uniprot/P12025 denotes MK
T6 1590-1592 http://www.uniprot.org/uniprot/P12025 denotes MK
T7 111-113 http://www.uniprot.org/uniprot/Q9R008 denotes MK
T8 457-459 http://www.uniprot.org/uniprot/Q9R008 denotes MK
T9 1073-1075 http://www.uniprot.org/uniprot/Q9R008 denotes MK
T10 1590-1592 http://www.uniprot.org/uniprot/Q9R008 denotes MK
T11 111-113 http://www.uniprot.org/uniprot/Q9QZM4 denotes MK
T12 457-459 http://www.uniprot.org/uniprot/Q9QZM4 denotes MK
T13 1073-1075 http://www.uniprot.org/uniprot/Q9QZM4 denotes MK
T14 1590-1592 http://www.uniprot.org/uniprot/Q9QZM4 denotes MK
T15 718-732 http://www.uniprot.org/uniprot/Q571E4 denotes chondroitinase

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 348-351 http://purl.bioontology.org/ontology/NCBITAXON/82391 denotes day

GO-BP

Id Subject Object Predicate Lexical cue
T1 87-93 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T2 134-140 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T3 206-214 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfates
T4 233-240 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T5 943-950 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T6 1110-1119 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfation
T7 1235-1243 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfated
T8 718-732 http://purl.obolibrary.org/obo/GO_0043890 denotes chondroitinase
T9 1283-1292 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion

GO-MF

Id Subject Object Predicate Lexical cue
T1 49-56 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T2 79-86 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T3 126-133 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T4 314-321 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T5 504-511 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T6 569-576 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T7 698-705 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T8 858-865 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T9 1165-1172 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T10 1369-1376 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T11 1414-1421 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T12 149-154 http://purl.obolibrary.org/obo/GO_0070026 denotes binds
T13 49-56 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T14 79-86 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T15 126-133 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T16 314-321 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T17 504-511 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T18 569-576 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T19 698-705 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T20 858-865 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T21 1165-1172 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T22 1369-1376 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T23 1414-1421 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T24 149-154 http://purl.obolibrary.org/obo/GO_0003680 denotes binds
T25 49-56 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T26 79-86 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T27 126-133 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T28 314-321 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T29 504-511 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T30 569-576 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T31 698-705 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T32 858-865 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T33 1165-1172 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T34 1369-1376 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T35 1414-1421 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T36 149-154 http://purl.obolibrary.org/obo/GO_0017091 denotes binds
T37 49-56 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T38 79-86 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T39 126-133 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T40 314-321 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T41 504-511 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T42 569-576 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T43 698-705 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T44 858-865 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T45 1165-1172 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T46 1369-1376 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T47 1414-1421 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T48 149-154 http://purl.obolibrary.org/obo/GO_0005488 denotes binds
T49 71-86 http://purl.obolibrary.org/obo/GO_0008201 denotes heparin-binding
T50 118-133 http://purl.obolibrary.org/obo/GO_0008201 denotes heparin-binding
T51 79-100 http://purl.obolibrary.org/obo/GO_0019838 denotes binding growth factor
T52 134-154 http://purl.obolibrary.org/obo/GO_0019838 denotes growth factor, binds
T53 79-100 http://purl.obolibrary.org/obo/GO_0048408 denotes binding growth factor
T54 134-154 http://purl.obolibrary.org/obo/GO_0048408 denotes growth factor, binds
T55 79-100 http://purl.obolibrary.org/obo/GO_0036458 denotes binding growth factor
T56 134-154 http://purl.obolibrary.org/obo/GO_0036458 denotes growth factor, binds
T57 79-100 http://purl.obolibrary.org/obo/GO_0017134 denotes binding growth factor
T58 134-154 http://purl.obolibrary.org/obo/GO_0017134 denotes growth factor, binds
T59 79-100 http://purl.obolibrary.org/obo/GO_0048406 denotes binding growth factor
T60 134-154 http://purl.obolibrary.org/obo/GO_0048406 denotes growth factor, binds
T61 79-100 http://purl.obolibrary.org/obo/GO_0070851 denotes binding growth factor
T62 134-154 http://purl.obolibrary.org/obo/GO_0070851 denotes growth factor, binds

UBERON-AE

Id Subject Object Predicate Lexical cue
T1 333-339 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brains
T2 358-365 http://purl.obolibrary.org/obo/UBERON_0000922 denotes embryos

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 259-271 http://edamontology.org/topic_0152 denotes carbohydrate
T2 1007-1012 http://edamontology.org/topic_2815 denotes human
T3 1056-1067 http://edamontology.org/topic_0602 denotes interaction
T4 1466-1473 http://edamontology.org/topic_3678 denotes studies

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 18-28 http://edamontology.org/data_0883 denotes structures
T2 180-190 http://edamontology.org/data_0883 denotes structures
T3 180-193 http://edamontology.org/data_1070 denotes structures in
T4 259-281 http://edamontology.org/data_1462 denotes carbohydrate structure
T5 272-281 http://edamontology.org/data_0883 denotes structure
T6 619-633 http://edamontology.org/data_2140 denotes concentrations
T7 892-901 http://edamontology.org/data_0883 denotes structure
T8 1021-1029 http://edamontology.org/operation_2945 denotes Analysis
T9 1216-1223 http://edamontology.org/operation_3432 denotes cluster
T10 1550-1559 http://edamontology.org/data_0883 denotes structure

GlycoBiology-MAT

Id Subject Object Predicate Lexical cue
T1 333-339 http://purl.obolibrary.org/obo/MAT_0000098 denotes brains
T2 358-365 http://purl.obolibrary.org/obo/MAT_0000226 denotes embryos

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 71-78 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T2 118-125 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T3 1521-1528 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T4 194-205 http://rdf.glycoinfo.org/glycan/G00018MO denotes chondroitin
T5 194-214 http://rdf.glycoinfo.org/glycan/G00018MO denotes chondroitin sulfates

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 409-416 id denotes labeled
PD-GlycoEpitope-B_T2 194-205 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin
PD-GlycoEpitope-B_T3 225-240 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 333-339 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brains
PD-UBERON-AE-B_T2 358-365 http://purl.obolibrary.org/obo/UBERON_0000922 denotes embryos

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 333-339 Body_part denotes brains http://purl.obolibrary.org/obo/MAT_0000098
T2 358-365 Body_part denotes embryos http://purl.obolibrary.org/obo/MAT_0000226

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 194-205 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT
T2 225-240 Glycan denotes heparan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 194-205 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081
T2 225-240 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 194-205 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT
T3 225-240 Glycan denotes heparan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 352-357 Organism denotes mouse 10088|10090
T3 1007-1012 Organism denotes human 9606

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-101 Sentence denotes Glycosaminoglycan structures required for strong binding to midkine, a heparin-binding growth factor.
T2 102-241 Sentence denotes Midkine (MK), a heparin-binding growth factor, binds strongly to oversulfated structures in chondroitin sulfates (CSs) and heparan sulfate.
T3 242-404 Sentence denotes To elucidate the carbohydrate structure actually involved in the strong binding, dissected brains from 13-day mouse embryos were incubated with [14C]-glucosamine.
T4 405-634 Sentence denotes The labeled glycosaminoglycans were fractionated by MK-agarose affinity chromatography to a weakly binding fraction, which was eluted by 0.5 M NaCl, and a strongly binding fraction, which was eluted by higher NaCl concentrations.
T5 635-875 Sentence denotes Among the unsaturated disaccharides released from the strongly binding fraction by chondroitinase ABC, DeltaDi-diSE with 4,6-disulfated N-acetylgalactosamine accounted for 32.3%, whereas its content was lower in the weakly binding fraction.
T6 876-1020 Sentence denotes Artificial CS-E structure was formed using N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase purified from squid or recombinant human enzyme.
T7 1021-1224 Sentence denotes Analysis of the products and their interaction with MK revealed that E units without 3-O-sulfation of glucuronic acid are sufficient for strong binding, provided that they are present as a dense cluster.
T8 1225-1431 Sentence denotes Among the sulfated disaccharides released by heparitinase digestion, the trisulfated one, DeltaDiHS-triS, was the most abundant in the strongly binding fraction and was lower in the weakly binding fraction.
T9 1432-1593 Sentence denotes Together with results of previous studies, we concluded that the multivalent trisulfated heparin-like unit is another structure involved in strong binding to MK.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 194-205 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081
T2 225-240 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 358-365 Body_part denotes embryos FMA:296970

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 333-339 Body_part denotes brains http://purl.obolibrary.org/obo/MAT_0000098
T2 358-365 Body_part denotes embryos http://purl.obolibrary.org/obo/MAT_0000226

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 352-357 OrganismTaxon denotes mouse 10088|10090
T3 1007-1012 OrganismTaxon denotes human 9606