PubMed:12629989
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/12629989","sourcedb":"PubMed","sourceid":"12629989","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/12629989","text":"Expression of transforming growth factor-beta 1 in hepatocellular carcinoma in comparison with the non-tumor tissue.\nBACKGROUND/AIMS: TGF-beta 1 (transforming growth factor-beta 1) has been shown to be overexpressed in various cancer cells including hepatocellular carcinoma cells. In this study, we examined the association of the immunohistochemical expression of TGF-beta 1 in hepatocellular carcinoma tissues with the clinicopathological findings.\nMETHODOLOGY: Thirty liver tumor biopsy specimens obtained with ultrasound guidance and in which tumor-tissue and non-tumor tissue existed were examined. TGF-beta 1 was stained using rabbit anti-human TGF-beta 1 antibody.\nRESULTS: TGF-beta 1 was expressed mainly in the cytoplasm and plasma membrane of the hepatocellular carcinoma cells, while it was stained in the fibrous septa and sinusoidal cells in the non-tumor tissue. When comparing the intensity of TGF-beta 1 staining in the hepatocellular carcinoma tissue with that in the non-tumor tissue, the former was more intense than the latter in 21 (70%) of the 30 cases. Although the expression of TGF-beta 1 in the hepatocellular carcinoma tissue was not related to the tumor size of hepatocellular carcinoma, it was correlated with the histological differentiation: the lower the histological differentiation grade of hepatocellular carcinoma was, the more intense was the TGF-beta 1 staining in hepatocellular carcinoma tissue. In addition, TGF-beta 1 tended to be overexpressed in the trabecular type of hepatocellular carcinoma. To elucidate the relationship of TGF-beta 1 staining with apoptosis in hepatocellular carcinoma cells, we performed terminal deoxynucleotidyl transferase-mediated DUTP biotinnick end labeling (TUNEL) in 11 of the 30 specimens. No positive cells for TUNEL were detected in the hepatocellular carcinoma tissues.\nCONCLUSIONS: These results indicated that the expression of TGF-beta 1 was associated with the histological differentiation of hepatocellular carcinoma cells.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":682,"end":692},"obj":"gene:7040"},{"id":"T1","span":{"begin":758,"end":782},"obj":"disease:C2239176"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"12629989-0#14#47#gene7040","span":{"begin":754,"end":1114},"obj":"gene7040"},{"id":"12629989-0#51#75#diseaseC2239176","span":{"begin":1119,"end":1146},"obj":"diseaseC2239176"}],"relations":[{"id":"14#47#gene704051#75#diseaseC2239176","pred":"associated_with","subj":"12629989-0#14#47#gene7040","obj":"12629989-0#51#75#diseaseC2239176"}],"attributes":[{"subj":"12629989-0#14#47#gene7040","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"12629989-0#51#75#diseaseC2239176","pred":"source","obj":"DisGeNET5_gene_disease"}]},{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":134,"end":451},"obj":"OBJECTIVE"},{"id":"T2","span":{"begin":465,"end":672},"obj":"METHODS"},{"id":"T3","span":{"begin":682,"end":1849},"obj":"RESULTS"},{"id":"T4","span":{"begin":1863,"end":2008},"obj":"CONCLUSIONS"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T3","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T4","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#939cec","default":true},{"id":"DisGeNET5_gene_disease","color":"#b6ec93"},{"id":"PubMed_Structured_Abstracts","color":"#ec93d0"}]}]}}