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PubMed:12553051 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 1033-1038 gene:2597 denotes GAPDH
T1 1121-1136 disease:C0007102 denotes colonic cancers
T2 1043-1053 gene:60 denotes beta-actin
T3 1121-1136 disease:C0007102 denotes colonic cancers
T4 1043-1053 gene:728378 denotes beta-actin
T5 1121-1136 disease:C0007102 denotes colonic cancers
R1 T0 T1 associated_with GAPDH,colonic cancers
R2 T2 T3 associated_with beta-actin,colonic cancers
R3 T4 T5 associated_with beta-actin,colonic cancers

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 95-477 BACKGROUND denotes GAPDH, beta-actin and 18S rRNA are widely employed as internal control genes, with the assumption that they are expressed constitutively to similar degrees in different cells and tissues and under different experimental conditions. In this study, we tested this assumption by assessment of the transcription of these three genes in human colonic tissues using a quantitative RT-PCR.
T2 487-961 RESULTS denotes GAPDH transcription was significantly greater in both colonic adenomas and cancers than in normal mucosa. In addition, transcription of beta-actin was significantly increased in cancers. The expression of 18S rRNA was essentially constant among these various tissues. Stable expression of 18S rRNA was observed during the growth of colonic cancer cells stimulated with serum, but both GAPDH and beta-actin transcription were up-regulated, coinciding with cell proliferation.
T3 974-1137 CONCLUSIONS denotes These results indicate that 18S rRNA is more reliable than GAPDH and beta-actin as an internal control gene for quantitative comparison of mRNA in colonic cancers.