PubMed:12493756
Annnotations
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 33-36 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T3 | 139-142 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T5 | 188-199 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
| T6 | 469-472 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T8 | 591-594 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T10 | 604-618 | Glycan_Motif | denotes | 9-O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74121RX |
| T11 | 615-618 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T13 | 716-719 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T15 | 734-737 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T17 | 1209-1212 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T19 | 1277-1280 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T21 | 1296-1310 | Glycan_Motif | denotes | 9-O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74121RX |
| T22 | 1307-1310 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T24 | 1347-1350 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T26 | 1541-1544 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T28 | 1574-1588 | Glycan_Motif | denotes | 9-O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74121RX |
| T29 | 1585-1588 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T31 | 1652-1655 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T33 | 1666-1669 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T35 | 1674-1688 | Glycan_Motif | denotes | 9-O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74121RX |
| T36 | 1685-1688 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T38 | 1705-1708 | Glycan_Motif | denotes | GM3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G91237TK|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G56516VH |
| T40 | 1788-1791 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T42 | 1993-1996 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T44 | 2036-2039 | Glycan_Motif | denotes | GD3 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ |
| T46 | 2251-2263 | Glycan_Motif | denotes | sialic acids | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
| T47 | 2303-2315 | Glycan_Motif | denotes | sialic acids | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-77 | Sentence | denotes | Genes modulated by expression of GD3 synthase in Chinese hamster ovary cells. |
| T2 | 78-159 | Sentence | denotes | Evidence that the Tis21 gene is involved in the induction of GD3 9-O-acetylation. |
| T3 | 160-292 | Sentence | denotes | 9-O-Acetylation is a common sialic acid modification, expressed in a developmentally regulated and tissue/cell type-specific manner. |
| T4 | 293-422 | Sentence | denotes | The relevant 9-O-acetyltransferase(s) have not been isolated or cloned; nor have mechanisms for their regulation been elucidated. |
| T5 | 423-619 | Sentence | denotes | We previously showed that transfection of the GD3 synthase (ST8Sia-I) gene into Chinese hamster ovary (CHO)-K1 cells gave expression of not only the disialoganglioside GD3 but also 9-O-acetyl-GD3. |
| T6 | 620-857 | Sentence | denotes | We now use differential display PCR between wild type CHO-K1 cells and clones stably expressing GD3 synthase (CHO-GD3 cells) to detect any increased expression of other genes and explore the possible induction of a 9-O-acetyltransferase. |
| T7 | 858-1025 | Sentence | denotes | The four CHO mRNAs showing major up-regulation were homologous to VCAM-1, Tis21, the KC-protein-like protein, and a functionally unknown type II transmembrane protein. |
| T8 | 1026-1102 | Sentence | denotes | A moderate increase in expression of the FxC1 and SPR-1 genes was also seen. |
| T9 | 1103-1253 | Sentence | denotes | Interestingly, these are different from genes observed by others to be up-regulated after transfection of GD3 synthase into a neuroblastoma cell line. |
| T10 | 1254-1359 | Sentence | denotes | We also isolated a CHO-GD3 mutant lacking 9-O-acetyl-GD3 following chemical mutagenesis (CHO-GD3-OAc(-)). |
| T11 | 1360-1492 | Sentence | denotes | Analysis of the above differential display PCR-derived genes in these cells showed that expression of Tis21 was selectively reduced. |
| T12 | 1493-1600 | Sentence | denotes | Transfection of a mouse Tis21 cDNA into the CHO-GD3-OAc(-) mutant cells restored 9-O-acetyl-GD3 expression. |
| T13 | 1601-1848 | Sentence | denotes | Since the only major gangliosides expressed by CHO-GD3 cells are GD3 and 9-O-acetyl-GD3 (in addition to GM3, the predominant ganglioside type in wild-type CHO-K1 cells), we conclude that GD3 enhances its own 9-O-acetylation via induction of Tis21. |
| T14 | 1849-2006 | Sentence | denotes | This is the first known nuclear inducible factor for 9-O-acetylation and also the first proof that 9-O-acetylation can be directly regulated by GD3 synthase. |
| T15 | 2007-2175 | Sentence | denotes | Finally, transfection of CHO-GD3-OAc(-) mutant cells with ST6Gal-I induced 9-O-acetylation specifically on sialylated N-glycans, in a manner similar to wild-type cells. |
| T16 | 2176-2329 | Sentence | denotes | This indicates separate machineries for 9-O-acetylation on alpha2-8-linked sialic acids of gangliosides and on alpha2-6-linked sialic acids on N-glycans. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 33-36 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T2 | 33-36 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T3 | 139-142 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T4 | 139-142 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T5 | 188-199 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
| T6 | 469-472 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T7 | 469-472 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T8 | 591-594 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T9 | 591-594 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T10 | 604-618 | https://glytoucan.org/Structures/Glycans/G74121RX | denotes | 9-O-acetyl-GD3 |
| T11 | 615-618 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T12 | 615-618 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T13 | 716-719 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T14 | 716-719 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T15 | 734-737 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T16 | 734-737 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T17 | 1209-1212 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T18 | 1209-1212 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T19 | 1277-1280 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T20 | 1277-1280 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T21 | 1296-1310 | https://glytoucan.org/Structures/Glycans/G74121RX | denotes | 9-O-acetyl-GD3 |
| T22 | 1307-1310 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T23 | 1307-1310 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T24 | 1347-1350 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T25 | 1347-1350 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T26 | 1541-1544 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T27 | 1541-1544 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T28 | 1574-1588 | https://glytoucan.org/Structures/Glycans/G74121RX | denotes | 9-O-acetyl-GD3 |
| T29 | 1585-1588 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T30 | 1585-1588 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T31 | 1652-1655 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T32 | 1652-1655 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T33 | 1666-1669 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T34 | 1666-1669 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T35 | 1674-1688 | https://glytoucan.org/Structures/Glycans/G74121RX | denotes | 9-O-acetyl-GD3 |
| T36 | 1685-1688 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T37 | 1685-1688 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T38 | 1705-1708 | https://glytoucan.org/Structures/Glycans/G56516VH | denotes | GM3 |
| T39 | 1705-1708 | https://glytoucan.org/Structures/Glycans/G91237TK | denotes | GM3 |
| T40 | 1788-1791 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T41 | 1788-1791 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T42 | 1993-1996 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T43 | 1993-1996 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T44 | 2036-2039 | https://glytoucan.org/Structures/Glycans/G81928IQ | denotes | GD3 |
| T45 | 2036-2039 | https://glytoucan.org/Structures/Glycans/G98544DH | denotes | GD3 |
| T46 | 2251-2263 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acids |
| T47 | 2303-2315 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acids |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 604-618 | http://www.glycoepitope.jp/epitopes/EP0064 | denotes | 9-O-acetyl-GD3 |
| T2 | 1296-1310 | http://www.glycoepitope.jp/epitopes/EP0064 | denotes | 9-O-acetyl-GD3 |
| T3 | 1574-1588 | http://www.glycoepitope.jp/epitopes/EP0064 | denotes | 9-O-acetyl-GD3 |
| T4 | 1674-1688 | http://www.glycoepitope.jp/epitopes/EP0064 | denotes | 9-O-acetyl-GD3 |
PennBioIE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 33-45 | protein | denotes | GD3 synthase |
| T2 | 96-101 | protein | denotes | Tis21 |
| T3 | 139-142 | protein | denotes | GD3 |
| T4 | 306-330 | protein | denotes | 9-O-acetyltransferase(s) |
| T5 | 469-481 | protein | denotes | GD3 synthase |
| T6 | 483-491 | protein | denotes | ST8Sia-I |
| T7 | 591-594 | protein | denotes | GD3 |
| T8 | 604-618 | protein | denotes | 9-O-acetyl-GD3 |
| T9 | 716-728 | protein | denotes | GD3 synthase |
| T10 | 835-856 | protein | denotes | 9-O-acetyltransferase |
| T11 | 867-870 | protein | denotes | CHO |
| T12 | 924-930 | protein | denotes | VCAM-1 |
| T13 | 932-937 | protein | denotes | Tis21 |
| T14 | 943-966 | protein | denotes | KC-protein-like protein |
| T15 | 987-1024 | protein | denotes | unknown type II transmembrane protein |
| T16 | 1067-1071 | protein | denotes | FxC1 |
| T17 | 1076-1081 | protein | denotes | SPR-1 |
| T18 | 1209-1221 | protein | denotes | GD3 synthase |
| T19 | 1229-1242 | protein | denotes | neuroblastoma |
| T20 | 1296-1310 | protein | denotes | 9-O-acetyl-GD3 |
| T21 | 1462-1467 | protein | denotes | Tis21 |
| T22 | 1517-1522 | protein | denotes | Tis21 |
| T23 | 1574-1588 | protein | denotes | 9-O-acetyl-GD3 |
| T24 | 1666-1669 | protein | denotes | GD3 |
| T25 | 1674-1688 | protein | denotes | 9-O-acetyl-GD3 |
| T26 | 1842-1847 | protein | denotes | Tis21 |
| T27 | 1993-2005 | protein | denotes | GD3 synthase |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 1229-1242 | Phenotype | denotes | neuroblastoma | HP:0003006 |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 1229-1242 | Disease | denotes | neuroblastoma | http://purl.obolibrary.org/obo/MONDO_0005072 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 33-36 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T2 | 139-142 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T3 | 469-472 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T4 | 591-594 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T5 | 606-618 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T6 | 716-719 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T7 | 734-737 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T8 | 1209-1212 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T9 | 1277-1280 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T10 | 1298-1310 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T11 | 1347-1350 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T12 | 1541-1544 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T13 | 1576-1588 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T14 | 1652-1655 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T15 | 1666-1669 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T16 | 1676-1688 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T17 | 1705-1708 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T18 | 1788-1791 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T19 | 1993-1996 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T20 | 2036-2039 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 604-618 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T2 | 1296-1310 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T3 | 1574-1588 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T4 | 1674-1688 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
GlyCosmos15-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 1229-1242 | Phenotype | denotes | neuroblastoma | HP:0003006 |
GlyCosmos15-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 1229-1242 | Disease | denotes | neuroblastoma | http://purl.obolibrary.org/obo/MONDO_0005072 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 49-64 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T2 | 503-518 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T3 | 1511-1516 | OrganismTaxon | denotes | mouse | 10088|10090 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 65-70 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
| T2 | 259-265 | Body_part | denotes | tissue | http://purl.obolibrary.org/obo/UBERON_0000479 |
| T3 | 519-524 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
| T4 | 1003-1016 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-77 | Sentence | denotes | Genes modulated by expression of GD3 synthase in Chinese hamster ovary cells. |
| T2 | 78-159 | Sentence | denotes | Evidence that the Tis21 gene is involved in the induction of GD3 9-O-acetylation. |
| T3 | 160-292 | Sentence | denotes | 9-O-Acetylation is a common sialic acid modification, expressed in a developmentally regulated and tissue/cell type-specific manner. |
| T4 | 293-422 | Sentence | denotes | The relevant 9-O-acetyltransferase(s) have not been isolated or cloned; nor have mechanisms for their regulation been elucidated. |
| T5 | 423-619 | Sentence | denotes | We previously showed that transfection of the GD3 synthase (ST8Sia-I) gene into Chinese hamster ovary (CHO)-K1 cells gave expression of not only the disialoganglioside GD3 but also 9-O-acetyl-GD3. |
| T6 | 620-857 | Sentence | denotes | We now use differential display PCR between wild type CHO-K1 cells and clones stably expressing GD3 synthase (CHO-GD3 cells) to detect any increased expression of other genes and explore the possible induction of a 9-O-acetyltransferase. |
| T7 | 858-1025 | Sentence | denotes | The four CHO mRNAs showing major up-regulation were homologous to VCAM-1, Tis21, the KC-protein-like protein, and a functionally unknown type II transmembrane protein. |
| T8 | 1026-1102 | Sentence | denotes | A moderate increase in expression of the FxC1 and SPR-1 genes was also seen. |
| T9 | 1103-1253 | Sentence | denotes | Interestingly, these are different from genes observed by others to be up-regulated after transfection of GD3 synthase into a neuroblastoma cell line. |
| T10 | 1254-1359 | Sentence | denotes | We also isolated a CHO-GD3 mutant lacking 9-O-acetyl-GD3 following chemical mutagenesis (CHO-GD3-OAc(-)). |
| T11 | 1360-1492 | Sentence | denotes | Analysis of the above differential display PCR-derived genes in these cells showed that expression of Tis21 was selectively reduced. |
| T12 | 1493-1600 | Sentence | denotes | Transfection of a mouse Tis21 cDNA into the CHO-GD3-OAc(-) mutant cells restored 9-O-acetyl-GD3 expression. |
| T13 | 1601-1848 | Sentence | denotes | Since the only major gangliosides expressed by CHO-GD3 cells are GD3 and 9-O-acetyl-GD3 (in addition to GM3, the predominant ganglioside type in wild-type CHO-K1 cells), we conclude that GD3 enhances its own 9-O-acetylation via induction of Tis21. |
| T14 | 1849-2006 | Sentence | denotes | This is the first known nuclear inducible factor for 9-O-acetylation and also the first proof that 9-O-acetylation can be directly regulated by GD3 synthase. |
| T15 | 2007-2175 | Sentence | denotes | Finally, transfection of CHO-GD3-OAc(-) mutant cells with ST6Gal-I induced 9-O-acetylation specifically on sialylated N-glycans, in a manner similar to wild-type cells. |
| T16 | 2176-2329 | Sentence | denotes | This indicates separate machineries for 9-O-acetylation on alpha2-8-linked sialic acids of gangliosides and on alpha2-6-linked sialic acids on N-glycans. |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-77 | Sentence | denotes | Genes modulated by expression of GD3 synthase in Chinese hamster ovary cells. |
| T2 | 78-159 | Sentence | denotes | Evidence that the Tis21 gene is involved in the induction of GD3 9-O-acetylation. |
| T3 | 160-292 | Sentence | denotes | 9-O-Acetylation is a common sialic acid modification, expressed in a developmentally regulated and tissue/cell type-specific manner. |
| T4 | 293-422 | Sentence | denotes | The relevant 9-O-acetyltransferase(s) have not been isolated or cloned; nor have mechanisms for their regulation been elucidated. |
| T5 | 423-619 | Sentence | denotes | We previously showed that transfection of the GD3 synthase (ST8Sia-I) gene into Chinese hamster ovary (CHO)-K1 cells gave expression of not only the disialoganglioside GD3 but also 9-O-acetyl-GD3. |
| T6 | 620-857 | Sentence | denotes | We now use differential display PCR between wild type CHO-K1 cells and clones stably expressing GD3 synthase (CHO-GD3 cells) to detect any increased expression of other genes and explore the possible induction of a 9-O-acetyltransferase. |
| T7 | 858-1025 | Sentence | denotes | The four CHO mRNAs showing major up-regulation were homologous to VCAM-1, Tis21, the KC-protein-like protein, and a functionally unknown type II transmembrane protein. |
| T8 | 1026-1102 | Sentence | denotes | A moderate increase in expression of the FxC1 and SPR-1 genes was also seen. |
| T9 | 1103-1253 | Sentence | denotes | Interestingly, these are different from genes observed by others to be up-regulated after transfection of GD3 synthase into a neuroblastoma cell line. |
| T10 | 1254-1359 | Sentence | denotes | We also isolated a CHO-GD3 mutant lacking 9-O-acetyl-GD3 following chemical mutagenesis (CHO-GD3-OAc(-)). |
| T11 | 1360-1492 | Sentence | denotes | Analysis of the above differential display PCR-derived genes in these cells showed that expression of Tis21 was selectively reduced. |
| T12 | 1493-1600 | Sentence | denotes | Transfection of a mouse Tis21 cDNA into the CHO-GD3-OAc(-) mutant cells restored 9-O-acetyl-GD3 expression. |
| T13 | 1601-1848 | Sentence | denotes | Since the only major gangliosides expressed by CHO-GD3 cells are GD3 and 9-O-acetyl-GD3 (in addition to GM3, the predominant ganglioside type in wild-type CHO-K1 cells), we conclude that GD3 enhances its own 9-O-acetylation via induction of Tis21. |
| T14 | 1849-2006 | Sentence | denotes | This is the first known nuclear inducible factor for 9-O-acetylation and also the first proof that 9-O-acetylation can be directly regulated by GD3 synthase. |
| T15 | 2007-2175 | Sentence | denotes | Finally, transfection of CHO-GD3-OAc(-) mutant cells with ST6Gal-I induced 9-O-acetylation specifically on sialylated N-glycans, in a manner similar to wild-type cells. |
| T16 | 2176-2329 | Sentence | denotes | This indicates separate machineries for 9-O-acetylation on alpha2-8-linked sialic acids of gangliosides and on alpha2-6-linked sialic acids on N-glycans. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 33-36 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T2 | 139-142 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T3 | 469-472 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T4 | 591-594 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T5 | 606-618 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T6 | 716-719 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T7 | 734-737 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T8 | 1209-1212 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T9 | 1277-1280 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T10 | 1298-1310 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T11 | 1347-1350 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T12 | 1541-1544 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T13 | 1576-1588 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T14 | 1652-1655 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T15 | 1666-1669 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T16 | 1676-1688 | Glycan | denotes | O-acetyl-GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G74121RX |
| T17 | 1705-1708 | Glycan | denotes | GM3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK |
| T18 | 1788-1791 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T19 | 1993-1996 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
| T20 | 2036-2039 | Glycan | denotes | GD3 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 604-618 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T2 | 1296-1310 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T3 | 1574-1588 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
| T4 | 1674-1688 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | 9-O-acetyl-GD3 | http://www.glycoepitope.jp/epitopes/EP0064 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 49-64 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T2 | 503-518 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T3 | 1511-1516 | OrganismTaxon | denotes | mouse | 10088|10090 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 65-70 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
| T2 | 259-265 | Body_part | denotes | tissue | http://purl.obolibrary.org/obo/UBERON_0000479 |
| T3 | 519-524 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
| T4 | 1003-1016 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |