PubMed:12483223 JSONTXT

{"project":"silkworm_phenotype","denotations":[{"id":"T15","span":{"begin":695,"end":710},"obj":"BMO_00548"},{"id":"T13","span":{"begin":448,"end":463},"obj":"BMO_00548"},{"id":"T9","span":{"begin":695,"end":710},"obj":"Gene:693047"},{"id":"T8","span":{"begin":510,"end":518},"obj":"Species:7091"},{"id":"T7","span":{"begin":448,"end":463},"obj":"Gene:693047"},{"id":"T6","span":{"begin":303,"end":315},"obj":"Chemical:-"},{"id":"T5","span":{"begin":260,"end":265},"obj":"Species:9606"},{"id":"T4","span":{"begin":175,"end":180},"obj":"Species:9606"},{"id":"T3","span":{"begin":121,"end":130},"obj":"Species:7091"},{"id":"T2","span":{"begin":41,"end":46},"obj":"Species:9606"},{"id":"T10","span":{"begin":1050,"end":1059},"obj":"Species:7091"},{"id":"T1","span":{"begin":11,"end":20},"obj":"Species:7091"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":11,"end":20},"obj":"Species:7091"},{"id":"T2","span":{"begin":41,"end":46},"obj":"Species:9606"},{"id":"T3","span":{"begin":121,"end":130},"obj":"Species:7091"},{"id":"T4","span":{"begin":175,"end":180},"obj":"Species:9606"},{"id":"T5","span":{"begin":260,"end":265},"obj":"Species:9606"},{"id":"T6","span":{"begin":303,"end":315},"obj":"Chemical:-"},{"id":"T7","span":{"begin":448,"end":463},"obj":"Gene:693047"},{"id":"T8","span":{"begin":510,"end":518},"obj":"Species:7091"},{"id":"T9","span":{"begin":695,"end":710},"obj":"Gene:693047"},{"id":"T10","span":{"begin":1050,"end":1059},"obj":"Species:7091"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":11,"end":20},"obj":"Species:7091"},{"id":"T10","span":{"begin":1050,"end":1059},"obj":"Species:7091"},{"id":"T2","span":{"begin":41,"end":46},"obj":"Species:9606"},{"id":"T3","span":{"begin":121,"end":130},"obj":"Species:7091"},{"id":"T4","span":{"begin":175,"end":180},"obj":"Species:9606"},{"id":"T5","span":{"begin":260,"end":265},"obj":"Species:9606"},{"id":"T6","span":{"begin":303,"end":315},"obj":"Chemical:-"},{"id":"T7","span":{"begin":448,"end":463},"obj":"Gene:693047"},{"id":"T8","span":{"begin":510,"end":518},"obj":"Species:7091"},{"id":"T9","span":{"begin":695,"end":710},"obj":"Gene:693047"},{"id":"T13","span":{"begin":448,"end":463},"obj":"BMO_00548"},{"id":"T15","span":{"begin":695,"end":710},"obj":"BMO_00548"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":519,"end":523},"obj":"Body_part"},{"id":"T2","span":{"begin":615,"end":621},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000213"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000021"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":11,"end":20},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":41,"end":46},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":121,"end":130},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":175,"end":180},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":260,"end":265},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":510,"end":518},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":1050,"end":1059},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"7091"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"7091"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9606"},{"id":"A5","pred":"db_id","subj":"T5","obj":"9606"},{"id":"A6","pred":"db_id","subj":"T6","obj":"7091"},{"id":"A7","pred":"db_id","subj":"T7","obj":"7091"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":610,"end":621},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0011146"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":992,"end":996},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000560"}],"text":"Transgenic silkworms produce recombinant human type III procollagen in cocoons.\nWe describe the generation of transgenic silkworms that produce cocoons containing recombinant human collagen. A fusion cDNA was constructed encoding a protein that incorporated a human type III procollagen mini-chain with C-propeptide deleted, a fibroin light chain (L-chain), and an enhanced green fluorescent protein (EGFP). This cDNA was ligated downstream of the fibroin L-chain promoter and inserted into a piggyBac vector. Silkworm eggs were injected with the vectors, producing worms displaying EGFP fluorescence in their silk glands. The cocoons emitted EGFP fluorescence, indicating that the promoter and fibroin L-chain cDNAs directed the synthesized products to be secreted into cocoons. The presence of fusion proteins in cocoons was demonstrated by immunoblotting, collagenase-sensitivity tests, and amino acid sequencing. The fusion proteins from cocoons were purified to a single electrophoretic band. This study demonstrates the viability of transgenic silkworms as a tool for producing useful proteins in bulk."}