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PubMed:12397072 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-242 Sentence denotes Nascent lipidated apolipoprotein B is transported to the Golgi as an incompletely folded intermediate as probed by its association with network of endoplasmic reticulum molecular chaperones, GRP94, ERp72, BiP, calreticulin, and cyclophilin B.
T2 243-401 Sentence denotes We have previously demonstrated that endoplasmic reticulum (ER)-resident molecular chaperones interact with apolipoprotein B-100 (apoB) during its maturation.
T3 402-552 Sentence denotes The initial stages of apoB folding occur while it is bound to the ER membrane, where it becomes partially lipidated to form a primordial intermediate.
T4 553-681 Sentence denotes We determined whether this intermediate is dependent on the assistance of molecular chaperones for its subsequent folding steps.
T5 682-812 Sentence denotes To that end, microsomes were prepared from HepG2 cells and luminal contents were subjected to KBr density gradient centrifugation.
T6 813-1039 Sentence denotes Immunoprecipitation of apoB followed by Western blotting showed that the luminal pool floated at a density of 1.12 g/ml and, like the membrane-bound pool, was associated with GRP94, ERp72, BiP, calreticulin, and cyclophilin B.
T7 1040-1297 Sentence denotes Except for calreticulin, chaperone/apoB ratio in the lumen was severalfold higher than that in the membrane, suggesting a role for these chaperones both in facilitating the release of the primordial intermediate into the ER lumen and in providing stability.
T8 1298-1538 Sentence denotes Subcellular fractionation on sucrose gradients showed that apoB in the Golgi was associated with the same array of chaperones as the pool of apoB recovered from heavy microsomes containing the ER, except that chaperone/apoB ratio was lower.
T9 1539-1739 Sentence denotes KBr density gradient fractionation showed that the major pool of luminal apoB in the Golgi was recovered from 1.02 < d < 1.08 g/ml, whereas apoB in ER was recovered primarily from 1.08 < d < 1.2 g/ml.
T10 1740-1808 Sentence denotes Both fractions were associated with the same spectrum of chaperones.
T11 1809-2124 Sentence denotes Together with the finding that GRP94 was found associated with sialylated apoB, we conclude that correct folding of apoB is dependent on the assistance of molecular chaperone, which play multiple roles in its maturation throughout the secretory pathway including distal compartments such as the trans-Golgi network.

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-242 Sentence denotes Nascent lipidated apolipoprotein B is transported to the Golgi as an incompletely folded intermediate as probed by its association with network of endoplasmic reticulum molecular chaperones, GRP94, ERp72, BiP, calreticulin, and cyclophilin B.
T2 243-401 Sentence denotes We have previously demonstrated that endoplasmic reticulum (ER)-resident molecular chaperones interact with apolipoprotein B-100 (apoB) during its maturation.
T3 402-552 Sentence denotes The initial stages of apoB folding occur while it is bound to the ER membrane, where it becomes partially lipidated to form a primordial intermediate.
T4 553-681 Sentence denotes We determined whether this intermediate is dependent on the assistance of molecular chaperones for its subsequent folding steps.
T5 682-812 Sentence denotes To that end, microsomes were prepared from HepG2 cells and luminal contents were subjected to KBr density gradient centrifugation.
T6 813-1297 Sentence denotes Immunoprecipitation of apoB followed by Western blotting showed that the luminal pool floated at a density of 1.12 g/ml and, like the membrane-bound pool, was associated with GRP94, ERp72, BiP, calreticulin, and cyclophilin B. Except for calreticulin, chaperone/apoB ratio in the lumen was severalfold higher than that in the membrane, suggesting a role for these chaperones both in facilitating the release of the primordial intermediate into the ER lumen and in providing stability.
T7 1298-1538 Sentence denotes Subcellular fractionation on sucrose gradients showed that apoB in the Golgi was associated with the same array of chaperones as the pool of apoB recovered from heavy microsomes containing the ER, except that chaperone/apoB ratio was lower.
T8 1539-1739 Sentence denotes KBr density gradient fractionation showed that the major pool of luminal apoB in the Golgi was recovered from 1.02 < d < 1.08 g/ml, whereas apoB in ER was recovered primarily from 1.08 < d < 1.2 g/ml.
T9 1740-1808 Sentence denotes Both fractions were associated with the same spectrum of chaperones.
T10 1809-2124 Sentence denotes Together with the finding that GRP94 was found associated with sialylated apoB, we conclude that correct folding of apoB is dependent on the assistance of molecular chaperone, which play multiple roles in its maturation throughout the secretory pathway including distal compartments such as the trans-Golgi network.

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 57-62 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794
T2 159-168 Body_part denotes reticulum http://purl.obolibrary.org/obo/UBERON_0007361
T3 292-301 Body_part denotes reticulum http://purl.obolibrary.org/obo/UBERON_0007361
T4 471-479 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 947-955 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T10 1093-1098 Body_part denotes lumen http://purl.obolibrary.org/obo/UBERON_0000464
T11 1139-1147 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T14 1264-1269 Body_part denotes lumen http://purl.obolibrary.org/obo/UBERON_0000464
T15 1369-1374 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794
T16 1624-1629 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794
T17 2110-2115 Body_part denotes Golgi http://purl.obolibrary.org/obo/GO_0005794