PubMed:12374875 / 511-1201 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
12374875-4#28#33#gene1673	28-33	gene1673	denotes	HBD-2
12374875-4#28#33#gene100289462	28-33	gene100289462	denotes	HBD-2
12374875-4#86#95#diseaseC0033860	86-95	diseaseC0033860	denotes	psoriasis
28#33#gene167386#95#diseaseC0033860	12374875-4#28#33#gene1673	12374875-4#86#95#diseaseC0033860	associated_with	HBD-2,psoriasis
28#33#gene10028946286#95#diseaseC0033860	12374875-4#28#33#gene100289462	12374875-4#86#95#diseaseC0033860	associated_with	HBD-2,psoriasis

Id	Subject	Object	Predicate	Lexical cue
T2	0-690	METHODS	denotes	The expression of LL-37 and HBD-2 protein in skin-biopsy specimens from patients with psoriasis, patients with atopic dermatitis, and normal subjects was determined by immunohistochemical analysis. The amount of antimicrobial peptides in extracts of skin samples was also analyzed by immunodot blot analysis (for LL-37) and Western blot analysis (for HBD-2). Quantitative, real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assays were used to confirm the relative expression of HBD-2 and LL-37 messenger RNA (mRNA) in the skin-biopsy specimens. These peptides were also tested for antimicrobial activity against Staphylococcus aureus with the use of a colony-forming assay.