> top > docs > PubMed:12187272 > annotations

PubMed:12187272 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-71 Sentence denotes Cyclooxygenase-2 expression is up-regulated in obstructed human ureter.
TextSentencer_T2 72-80 Sentence denotes PURPOSE:
TextSentencer_T3 81-194 Sentence denotes Prostanoids produce significant effects on ureteral function and are synthesized by cyclooxygenase (COX) enzymes.
TextSentencer_T4 195-284 Sentence denotes COX is found in the 2 isoforms COX-1 (a constitutive form) and COX-2 (an inducible form).
TextSentencer_T5 285-396 Sentence denotes Due to the side effects associated with COX-1 inhibition there is great interest in selective COX-2 inhibition.
TextSentencer_T6 397-502 Sentence denotes We determined if COX-2 messenger (m)RNA and protein expression are regulated during ureteral obstruction.
TextSentencer_T7 503-755 Sentence denotes MATERIALS AND METHODS: mRNA analysis was performed using excess ureteral segments from 6 patients undergoing reconstructive procedures for chronic ureteral obstruction and 8 (normal ureter) undergoing donor nephrectomy after providing informed consent.
TextSentencer_T8 756-833 Sentence denotes All ureteral segments were snap frozen in liquid nitrogen and stored at -70C.
TextSentencer_T9 834-1041 Sentence denotes RNA was isolated from the segments using phenol extraction and complementary DNA was synthesized by reverse transcription with murine leukemia virus reverse transcriptase (Promega Corp., Madison, Wisconsin).
TextSentencer_T10 1042-1215 Sentence denotes Semiquantitative reverse transcriptase-polymerase chain reaction was performed using specific COX-2 gene primers with ribosomal S26 primers serving as the housekeeping gene.
TextSentencer_T11 1216-1320 Sentence denotes The polymerase chain reaction product was quantified by agarose gel electrophoresis and phospho-imaging.
TextSentencer_T12 1321-1365 Sentence denotes The ratio of COX-2-to-S26 mRNA was compared.
TextSentencer_T13 1366-1552 Sentence denotes Additional segments were homogenized and total protein was extracted, separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes.
TextSentencer_T14 1553-1715 Sentence denotes These membranes were Western blotted for COX-2 and glyceraldehyde-3-phosphate dehydrogenase (housekeeping protein) with specific primary and secondary antibodies.
TextSentencer_T15 1716-1724 Sentence denotes RESULTS:
TextSentencer_T16 1725-1967 Sentence denotes The mean ratio of COX-2-to-S26 mRNA plus or minus standard error at 20, 22 and 24 cycles of amplification was 0.22 +/- 0.04 in the 8 normal ureters compared with 1.01 +/- 0.21 in the 6 obstructed ureters (unpaired Student's t test p = 0.004).
TextSentencer_T17 1968-2182 Sentence denotes Similarly the mean ratio of COX-2-to-glyceraldehyde-3-phosphate dehydrogenase protein on immunoblotting was 0.15 +/- 0.02 in the 8 normal ureters compared with 0.59 +/- 0.10 in the 6 obstructed ureters (p = 0.003).
TextSentencer_T18 2183-2195 Sentence denotes CONCLUSIONS:
TextSentencer_T19 2196-2308 Sentence denotes These data indicate that COX-2 mRNA and protein levels are up-regulated in chronically obstructed human ureters.
TextSentencer_T20 2309-2433 Sentence denotes Using selective COX-2 inhibitors may be useful for treating prostanoid induced effects associated with ureteral obstruction.
T1 0-71 Sentence denotes Cyclooxygenase-2 expression is up-regulated in obstructed human ureter.
T2 72-80 Sentence denotes PURPOSE:
T3 81-194 Sentence denotes Prostanoids produce significant effects on ureteral function and are synthesized by cyclooxygenase (COX) enzymes.
T4 195-284 Sentence denotes COX is found in the 2 isoforms COX-1 (a constitutive form) and COX-2 (an inducible form).
T5 285-396 Sentence denotes Due to the side effects associated with COX-1 inhibition there is great interest in selective COX-2 inhibition.
T6 397-502 Sentence denotes We determined if COX-2 messenger (m)RNA and protein expression are regulated during ureteral obstruction.
T7 503-755 Sentence denotes MATERIALS AND METHODS: mRNA analysis was performed using excess ureteral segments from 6 patients undergoing reconstructive procedures for chronic ureteral obstruction and 8 (normal ureter) undergoing donor nephrectomy after providing informed consent.
T8 756-833 Sentence denotes All ureteral segments were snap frozen in liquid nitrogen and stored at -70C.
T9 834-1041 Sentence denotes RNA was isolated from the segments using phenol extraction and complementary DNA was synthesized by reverse transcription with murine leukemia virus reverse transcriptase (Promega Corp., Madison, Wisconsin).
T10 1042-1215 Sentence denotes Semiquantitative reverse transcriptase-polymerase chain reaction was performed using specific COX-2 gene primers with ribosomal S26 primers serving as the housekeeping gene.
T11 1216-1320 Sentence denotes The polymerase chain reaction product was quantified by agarose gel electrophoresis and phospho-imaging.
T12 1321-1365 Sentence denotes The ratio of COX-2-to-S26 mRNA was compared.
T13 1366-1552 Sentence denotes Additional segments were homogenized and total protein was extracted, separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes.
T14 1553-1715 Sentence denotes These membranes were Western blotted for COX-2 and glyceraldehyde-3-phosphate dehydrogenase (housekeeping protein) with specific primary and secondary antibodies.
T15 1716-1724 Sentence denotes RESULTS:
T16 1725-1967 Sentence denotes The mean ratio of COX-2-to-S26 mRNA plus or minus standard error at 20, 22 and 24 cycles of amplification was 0.22 +/- 0.04 in the 8 normal ureters compared with 1.01 +/- 0.21 in the 6 obstructed ureters (unpaired Student's t test p = 0.004).
T17 1968-2182 Sentence denotes Similarly the mean ratio of COX-2-to-glyceraldehyde-3-phosphate dehydrogenase protein on immunoblotting was 0.15 +/- 0.02 in the 8 normal ureters compared with 0.59 +/- 0.10 in the 6 obstructed ureters (p = 0.003).
T18 2183-2195 Sentence denotes CONCLUSIONS:
T19 2196-2308 Sentence denotes These data indicate that COX-2 mRNA and protein levels are up-regulated in chronically obstructed human ureters.
T20 2309-2433 Sentence denotes Using selective COX-2 inhibitors may be useful for treating prostanoid induced effects associated with ureteral obstruction.

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12187272-16#16#21#gene4513 2325-2330 gene4513 denotes COX-2
12187272-16#16#21#gene5743 2325-2330 gene5743 denotes COX-2
12187272-16#103#123#diseaseC0041956 2412-2432 diseaseC0041956 denotes ureteral obstruction
16#21#gene4513103#123#diseaseC0041956 12187272-16#16#21#gene4513 12187272-16#103#123#diseaseC0041956 associated_with COX-2,ureteral obstruction
16#21#gene5743103#123#diseaseC0041956 12187272-16#16#21#gene5743 12187272-16#103#123#diseaseC0041956 associated_with COX-2,ureteral obstruction

DisGeNet-2017-sample

Id Subject Object Predicate Lexical cue
T1647 2325-2330 gene:4513 denotes COX-2
T1648 2412-2432 disease:C0041956 denotes ureteral obstruction
R1 T1647 T1648 associated_with COX-2,ureteral obstruction
R2 T1647 T1648 associated_with COX-2,ureteral obstruction

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 64-70 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureter
PD-UBERON-AE-B_T2 685-691 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureter
PD-UBERON-AE-B_T3 1865-1872 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T4 1921-1928 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T5 2106-2113 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T6 2162-2169 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T7 2300-2307 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 64-70 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureter
PD-UBERON-AE-B_T2 685-691 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureter
PD-UBERON-AE-B_T3 1865-1872 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T4 1921-1928 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T5 2106-2113 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T6 2162-2169 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters
PD-UBERON-AE-B_T7 2300-2307 http://purl.obolibrary.org/obo/UBERON_0000056 denotes ureters

DisGeNET

Id Subject Object Predicate Lexical cue
T0 414-419 gene:4513 denotes COX-2
T1 481-501 disease:C0041956 denotes ureteral obstruction
T2 414-419 gene:5743 denotes COX-2
T3 481-501 disease:C0041956 denotes ureteral obstruction
R1 T0 T1 associated_with COX-2,ureteral obstruction
R2 T2 T3 associated_with COX-2,ureteral obstruction