PubMed:1212667 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1212667","sourcedb":"PubMed","sourceid":"1212667","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1212667","text":"Multiple Smith-degradations of carcinoembryonic antigen (CEA) and of asialo CEA.\nCarcinoembryonic antigen (CEA) and asialo CEA were subjected to multiple Smith-degradation (i.e., for each degradation, application in sequence of periodate oxidation, borohydride reduction, and mild hydrolysis with acid; borohydride-t was substituted for unlabelled borohydride). High yields of modified glycoproteins were obtained at each stage. After three complete degradations and a further periodate-borohydride-t treatment, the carbohydrate content of CEA and of asialo CEA had decreased from 45-50% to 11-12% (i.e., 90% removal of carbohydrate). Glycerol was always one of the products obtained after each degradation, but threitol and erythritol were not detected. The second degradation caused a substantial loss of 2-acetamide-2-deoxyglucose, which is consistent with the location of some of this monosaccharide towards the terminal (non-reducing) end of the oligosaccharides. The \"core\" region of the oligosaccharides is composed of galactose, mannose, and 2-acetamido-2-deoxyglucose. After the fourth oxidation, 2-acetamido-2-deoxyglucose was 50-60% of the total content of residual carbohydrate. After the first degradation, there was a progressive loss in antigenic activity, but this was associated with a small amount of hydrolysis of the protein moiety of CEA.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":80},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":81,"end":361},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":362,"end":428},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":429,"end":634},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":635,"end":754},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":755,"end":968},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":969,"end":1077},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1078,"end":1190},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1191,"end":1359},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":80},"obj":"Sentence"},{"id":"T2","span":{"begin":81,"end":361},"obj":"Sentence"},{"id":"T3","span":{"begin":362,"end":428},"obj":"Sentence"},{"id":"T4","span":{"begin":429,"end":634},"obj":"Sentence"},{"id":"T5","span":{"begin":635,"end":754},"obj":"Sentence"},{"id":"T6","span":{"begin":755,"end":968},"obj":"Sentence"},{"id":"T7","span":{"begin":969,"end":1077},"obj":"Sentence"},{"id":"T8","span":{"begin":1078,"end":1190},"obj":"Sentence"},{"id":"T9","span":{"begin":1191,"end":1359},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":1026,"end":1035},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T2","span":{"begin":1026,"end":1035},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"},{"id":"T3","span":{"begin":1037,"end":1044},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":1026,"end":1035},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":1037,"end":1044},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ad93ec","default":true},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93ec93"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93ac"}]}]}}