PubMed:12044662
Annnotations
UseCases_ArguminSci_Discourse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-126 | DRI_Background | denotes | A gelatin in situ-overlay technique localizes brain matrix metalloproteinase activity in experimental focal cerebral ischemia. |
| T2 | 127-446 | DRI_Background | denotes | To determine the activity of matrix metalloproteinases (MMP), especially MMP-2 and MMP-9, which play an important role in ischemic stroke and intracerebral hemorrhage, we adapted a simple and rapid method for localizing gelatinase activity to a gelatin film in situ-overlay technique previously used in cancer research. |
| T3 | 447-624 | DRI_Background | denotes | Ten micrometer cryosections of rat brain from controls and animals subjected to 3 h of ischemia and 48 h of reperfusion (suture model for transient cerebral ischemia) were used. |
| T4 | 625-801 | DRI_Approach | denotes | After thawing, a gelatin film with a polyester base was put on the slide, incubated for 24 h at 37 degrees C, stained with Ponceau S, and then discolored in bi-distilled water. |
| T5 | 802-887 | DRI_Approach | denotes | Non-staining areas on the film corresponded to lysis zones, caused by activated MMPs. |
| T6 | 888-1078 | DRI_Approach | denotes | This was proven by MMP incubation at various concentrations on the plain gelatin film and pretreatment with EDTA (an MMP inhibitor), which prevents lysis zones in normal and ischemic brains. |
| T7 | 1079-1238 | DRI_Approach | denotes | As confirmatory tests, SDS-PAGE zymography was used to define MMP activity, and also MMP-2 immunohistochemistry to detect the possibly cellular origin of MMPs. |
| T8 | 1239-1369 | DRI_Background | denotes | Normal rat brain exhibited a low background activity, which was visible as a light halo-like lysis zone over and around the brain. |
| T9 | 1370-1501 | DRI_Background | denotes | Areas in normal brain with medium MMP activity were within the white matter (corpus callosum, anterior commissure, and cerebellum). |
| T10 | 1502-1635 | DRI_Background | denotes | Ischemic brain exhibited high activity lysis zones within the infarcted area (detected by microtubuli associated protein-2 staining). |
| T11 | 1636-1733 | DRI_Background | denotes | These zones consisted of microscopically small lysis holes with a diameter of about 10-20 microm. |
| T12 | 1734-1813 | DRI_Outcome | denotes | Immunohistochemistry showed that especially microvessels expressed MMP antigen. |
| T13 | 1814-1959 | DRI_Background | denotes | SDS-PAGE zymography differentiated between a high level of activated MMPs in the ischemic area and a low level in the non-ischemic basal ganglia. |
| T14 | 1960-2088 | DRI_Background | denotes | The gelatin film in situ-overlay technique is able to localize MMP activity in ischemic rat brain tissue on a microscopic level. |
c_corpus
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 2-9 | 4716 | denotes | gelatin |
| T2 | 2-9 | D005780 | denotes | gelatin |
| T3 | 2-9 | CHEBI:5291 | denotes | gelatin |
| T4 | 46-51 | UBERON:6110636 | denotes | brain |
| T5 | 46-51 | UBERON:0000955 | denotes | brain |
| T6 | 52-58 | UBERON:0027368 | denotes | matrix |
| T7 | 59-85 | GO:0008237 | denotes | metalloproteinase activity |
| T8 | 108-125 | D002545 | denotes | cerebral ischemia |
| T9 | 108-125 | D002545 | denotes | cerebral ischemia |
| T12 | 156-162 | UBERON:0027368 | denotes | matrix |
| T13 | 156-181 | D020782 | denotes | matrix metalloproteinases |
| T17 | 183-186 | PR:O04529 | denotes | MMP |
| T15 | 183-186 | CHEBI:340824 | denotes | MMP |
| T18 | 183-186 | CHEBI:59761 | denotes | MMP |
| T16 | 183-186 | GO:0004235 | denotes | MMP |
| T19 | 200-203 | CHEBI:340824 | denotes | MMP |
| T22 | 200-203 | CHEBI:59761 | denotes | MMP |
| T23 | 200-205 | PR:P08253 | denotes | MMP-2 |
| T24 | 200-205 | Q9GLE5 | denotes | MMP-2 |
| T25 | 200-205 | P08253 | denotes | MMP-2 |
| T26 | 200-205 | Q90611 | denotes | MMP-2 |
| T28 | 200-205 | PR:Q8MPP3 | denotes | MMP-2 |
| T29 | 200-205 | P33436 | denotes | MMP-2 |
| T30 | 200-205 | PR:Q90611 | denotes | MMP-2 |
| T31 | 200-205 | PR:P33436 | denotes | MMP-2 |
| T32 | 200-205 | PR:000010479 | denotes | MMP-2 |
| T33 | 200-205 | PR:P33434 | denotes | MMP-2 |
| T34 | 200-205 | P33434 | denotes | MMP-2 |
| T35 | 200-205 | P50757 | denotes | MMP-2 |
| T27 | 200-205 | GO:0004228 | denotes | MMP-2 |
| T36 | 210-213 | CHEBI:340824 | denotes | MMP |
| T39 | 210-213 | CHEBI:59761 | denotes | MMP |
| T40 | 210-215 | PR:P50282 | denotes | MMP-9 |
| T41 | 210-215 | PR:P14780 | denotes | MMP-9 |
| T42 | 210-215 | P14780 | denotes | MMP-9 |
| T43 | 210-215 | PR:P41245 | denotes | MMP-9 |
| T44 | 210-215 | P41246 | denotes | MMP-9 |
| T45 | 210-215 | P41245 | denotes | MMP-9 |
| T46 | 210-215 | PR:P52176 | denotes | MMP-9 |
| T47 | 210-215 | P50282 | denotes | MMP-9 |
| T49 | 210-215 | PR:000010491 | denotes | MMP-9 |
| T50 | 210-215 | P52176 | denotes | MMP-9 |
| T51 | 210-215 | O18733 | denotes | MMP-9 |
| T48 | 210-215 | GO:0004229 | denotes | MMP-9 |
| T52 | 269-293 | D002543 | denotes | intracerebral hemorrhage |
| T53 | 269-293 | D002543 | denotes | intracerebral hemorrhage |
| T57 | 347-357 | Q833V7 | denotes | gelatinase |
| T56 | 347-357 | D018093 | denotes | gelatinase |
| T59 | 372-379 | 4716 | denotes | gelatin |
| T62 | 372-384 | C015233 | denotes | gelatin film |
| T64 | 478-481 | PR:P63003-1 | denotes | rat |
| T65 | 478-481 | PR:Q2G0B1 | denotes | rat |
| T66 | 478-481 | PR:Q8VHJ4 | denotes | rat |
| T63 | 478-481 | 10116 | denotes | rat |
| T67 | 478-481 | D051381 | denotes | rat |
| T68 | 482-487 | UBERON:6110636 | denotes | brain |
| T69 | 482-487 | UBERON:0000955 | denotes | brain |
| T70 | 534-542 | D007511 | denotes | ischemia |
| T71 | 534-542 | D007511 | denotes | ischemia |
| T72 | 568-574 | UBERON:0003685 | denotes | suture |
| T73 | 568-574 | UBERON:4200215 | denotes | suture |
| T74 | 585-612 | D002546 | denotes | transient cerebral ischemia |
| T75 | 585-612 | D002546 | denotes | transient cerebral ischemia |
| T80 | 642-649 | 4716 | denotes | gelatin |
| T83 | 642-654 | C015233 | denotes | gelatin film |
| T84 | 662-671 | D011091 | denotes | polyester |
| T85 | 662-671 | CHEBI:60160 | denotes | polyester |
| T86 | 662-671 | CHEBI:26178 | denotes | polyester |
| T88 | 672-676 | SO:0001236 | denotes | base |
| T87 | 672-676 | CHEBI:22695 | denotes | base |
| T89 | 713-717 | CVCL_8262 | denotes | 24 h |
| T90 | 748-757 | C032756 | denotes | Ponceau S |
| T91 | 748-757 | C032756 | denotes | Ponceau S |
| T92 | 748-757 | CHEBI:90319 | denotes | Ponceau S |
| T93 | 849-854 | GO:0019835 | denotes | lysis |
| T94 | 882-886 | D020782 | denotes | MMPs |
| T97 | 907-910 | PR:O04529 | denotes | MMP |
| T95 | 907-910 | CHEBI:340824 | denotes | MMP |
| T98 | 907-910 | CHEBI:59761 | denotes | MMP |
| T96 | 907-910 | GO:0004235 | denotes | MMP |
| T99 | 961-968 | 4716 | denotes | gelatin |
| T102 | 961-973 | C015233 | denotes | gelatin film |
| T103 | 996-1000 | D004492 | denotes | EDTA |
| T104 | 996-1000 | CHEBI:42191 | denotes | EDTA |
| T105 | 996-1000 | CHEBI:64755 | denotes | EDTA |
| T106 | 996-1000 | D004492 | denotes | EDTA |
| T109 | 1005-1008 | PR:O04529 | denotes | MMP |
| T107 | 1005-1008 | CHEBI:340824 | denotes | MMP |
| T110 | 1005-1008 | CHEBI:59761 | denotes | MMP |
| T108 | 1005-1008 | GO:0004235 | denotes | MMP |
| T111 | 1009-1018 | CHEBI:35222 | denotes | inhibitor |
| T112 | 1036-1041 | GO:0019835 | denotes | lysis |
| T113 | 1102-1105 | PR:000014570 | denotes | SDS |
| T114 | 1102-1105 | B5HDJ6 | denotes | SDS |
| T115 | 1102-1105 | PR:000014571 | denotes | SDS |
| T116 | 1102-1105 | PR:P09367 | denotes | SDS |
| T117 | 1102-1105 | PR:P20132 | denotes | SDS |
| T118 | 1102-1105 | PR:Q8R238 | denotes | SDS |
| T121 | 1102-1105 | PR:Q8VBT2 | denotes | SDS |
| T122 | 1102-1105 | PR:Q86B06 | denotes | SDS |
| T123 | 1102-1105 | PR:Q1PFW3 | denotes | SDS |
| T120 | 1102-1105 | CHEBI:8984 | denotes | SDS |
| T119 | 1102-1105 | D000081003 | denotes | SDS |
| T126 | 1141-1144 | PR:O04529 | denotes | MMP |
| T124 | 1141-1144 | CHEBI:340824 | denotes | MMP |
| T127 | 1141-1144 | CHEBI:59761 | denotes | MMP |
| T125 | 1141-1144 | GO:0004235 | denotes | MMP |
| T128 | 1164-1167 | CHEBI:340824 | denotes | MMP |
| T131 | 1164-1167 | CHEBI:59761 | denotes | MMP |
| T132 | 1164-1169 | PR:P08253 | denotes | MMP-2 |
| T133 | 1164-1169 | Q9GLE5 | denotes | MMP-2 |
| T134 | 1164-1169 | P08253 | denotes | MMP-2 |
| T135 | 1164-1169 | Q90611 | denotes | MMP-2 |
| T137 | 1164-1169 | PR:Q8MPP3 | denotes | MMP-2 |
| T138 | 1164-1169 | P33436 | denotes | MMP-2 |
| T139 | 1164-1169 | PR:Q90611 | denotes | MMP-2 |
| T140 | 1164-1169 | PR:P33436 | denotes | MMP-2 |
| T141 | 1164-1169 | PR:000010479 | denotes | MMP-2 |
| T142 | 1164-1169 | PR:P33434 | denotes | MMP-2 |
| T143 | 1164-1169 | P33434 | denotes | MMP-2 |
| T144 | 1164-1169 | P50757 | denotes | MMP-2 |
| T136 | 1164-1169 | GO:0004228 | denotes | MMP-2 |
| T145 | 1233-1237 | D020782 | denotes | MMPs |
| T147 | 1246-1249 | PR:P63003-1 | denotes | rat |
| T148 | 1246-1249 | PR:Q2G0B1 | denotes | rat |
| T149 | 1246-1249 | PR:Q8VHJ4 | denotes | rat |
| T146 | 1246-1249 | 10116 | denotes | rat |
| T150 | 1246-1249 | D051381 | denotes | rat |
| T151 | 1250-1255 | UBERON:6110636 | denotes | brain |
| T152 | 1250-1255 | UBERON:0000955 | denotes | brain |
| T153 | 1322-1326 | GO:1990038 | denotes | halo |
| T154 | 1332-1337 | GO:0019835 | denotes | lysis |
| T155 | 1359-1368 | UBERON:0000955 | denotes | the brain |
| T158 | 1386-1391 | UBERON:6110636 | denotes | brain |
| T159 | 1386-1391 | UBERON:0000955 | denotes | brain |
| T162 | 1404-1407 | PR:O04529 | denotes | MMP |
| T160 | 1404-1407 | CHEBI:340824 | denotes | MMP |
| T163 | 1404-1407 | CHEBI:59761 | denotes | MMP |
| T161 | 1404-1407 | GO:0004235 | denotes | MMP |
| T166 | 1433-1445 | UBERON:0002316 | denotes | white matter |
| T164 | 1433-1445 | D056784 | denotes | white matter |
| T165 | 1433-1445 | D056784 | denotes | white matter |
| T169 | 1447-1462 | UBERON:0002336 | denotes | corpus callosum |
| T170 | 1464-1483 | UBERON:0004170 | denotes | anterior commissure |
| T171 | 1464-1483 | UBERON:0000935 | denotes | anterior commissure |
| T173 | 1489-1499 | UBERON:0002037 | denotes | cerebellum |
| T174 | 1511-1516 | UBERON:6110636 | denotes | brain |
| T175 | 1511-1516 | UBERON:0000955 | denotes | brain |
| T176 | 1541-1546 | GO:0019835 | denotes | lysis |
| T177 | 1592-1603 | GO:0005874 | denotes | microtubuli |
| T182 | 1615-1622 | SO:0000104 | denotes | protein |
| T178 | 1615-1622 | GO:0003675 | denotes | protein |
| T179 | 1615-1622 | CHEBI:36080 | denotes | protein |
| T180 | 1615-1622 | CHEBI:11122 | denotes | protein |
| T183 | 1615-1624 | Q02966 | denotes | protein-2 |
| T184 | 1615-1624 | Q68Y31 | denotes | protein-2 |
| T185 | 1615-1624 | P03548 | denotes | protein-2 |
| T186 | 1615-1624 | Q00965 | denotes | protein-2 |
| T187 | 1615-1624 | P03550 | denotes | protein-2 |
| T188 | 1615-1624 | P05397 | denotes | protein-2 |
| T189 | 1615-1624 | P19818 | denotes | protein-2 |
| T190 | 1615-1624 | P03549 | denotes | protein-2 |
| T191 | 1615-1624 | P09521 | denotes | protein-2 |
| T192 | 1615-1624 | Q01087 | denotes | protein-2 |
| T193 | 1615-1624 | Q8V3T8 | denotes | protein-2 |
| T194 | 1683-1688 | GO:0019835 | denotes | lysis |
| T197 | 1801-1804 | PR:O04529 | denotes | MMP |
| T195 | 1801-1804 | CHEBI:340824 | denotes | MMP |
| T198 | 1801-1804 | CHEBI:59761 | denotes | MMP |
| T196 | 1801-1804 | GO:0004235 | denotes | MMP |
| T199 | 1805-1812 | D000941 | denotes | antigen |
| T200 | 1805-1812 | CHEBI:59132 | denotes | antigen |
| T201 | 1805-1812 | D000941 | denotes | antigen |
| T202 | 1814-1817 | PR:000014570 | denotes | SDS |
| T203 | 1814-1817 | B5HDJ6 | denotes | SDS |
| T204 | 1814-1817 | PR:000014571 | denotes | SDS |
| T205 | 1814-1817 | PR:P09367 | denotes | SDS |
| T206 | 1814-1817 | PR:P20132 | denotes | SDS |
| T207 | 1814-1817 | PR:Q8R238 | denotes | SDS |
| T210 | 1814-1817 | PR:Q8VBT2 | denotes | SDS |
| T211 | 1814-1817 | PR:Q86B06 | denotes | SDS |
| T212 | 1814-1817 | PR:Q1PFW3 | denotes | SDS |
| T209 | 1814-1817 | CHEBI:8984 | denotes | SDS |
| T208 | 1814-1817 | D000081003 | denotes | SDS |
| T213 | 1883-1887 | D020782 | denotes | MMPs |
| T214 | 1945-1958 | UBERON:0002420 | denotes | basal ganglia |
| T217 | 1945-1958 | UBERON:0006098 | denotes | basal ganglia |
| T218 | 1945-1958 | UBERON:0010011 | denotes | basal ganglia |
| T215 | 1945-1958 | D001480 | denotes | basal ganglia |
| T216 | 1945-1958 | D001480 | denotes | basal ganglia |
| T220 | 1964-1971 | 4716 | denotes | gelatin |
| T223 | 1964-1976 | C015233 | denotes | gelatin film |
| T226 | 2023-2026 | PR:O04529 | denotes | MMP |
| T224 | 2023-2026 | CHEBI:340824 | denotes | MMP |
| T227 | 2023-2026 | CHEBI:59761 | denotes | MMP |
| T225 | 2023-2026 | GO:0004235 | denotes | MMP |
| T229 | 2048-2051 | PR:P63003-1 | denotes | rat |
| T230 | 2048-2051 | PR:Q2G0B1 | denotes | rat |
| T231 | 2048-2051 | PR:Q8VHJ4 | denotes | rat |
| T228 | 2048-2051 | 10116 | denotes | rat |
| T232 | 2048-2051 | D051381 | denotes | rat |
| T233 | 2052-2057 | UBERON:6110636 | denotes | brain |
| T234 | 2052-2057 | UBERON:0000955 | denotes | brain |
| T235 | 2058-2064 | UBERON:0000479 | denotes | tissue |