PubMed:12007471 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/12007471","sourcedb":"PubMed","sourceid":"12007471","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/12007471","text":"Influence of exo beta-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure.\nWe have examined beta-D-galactofuranosidase production by Penicillium fellutanum in the presence of D-galactono-1,4-lactone or 4-aminophenyl 1-thio-beta-D-galactofuranoside, two potent in vitro inhibitors of the enzyme. Activity of the enzyme in the culture filtrate was increased by 35% when glucose was replaced by D-galactose as the carbon source, and the activity diminished 80% of the control value when the inhibitors were added. Significant alterations of the culture were observed: (a) the medium became increasingly opalescent due to the secretion of a protein aggregate (PA) which contained 15% neutral sugar, mainly ribose; (b) the peptidophosphogalactomannan (pPGM) containing galactofuranose, normally produced by P. fellutanum, could not be obtained from the culture medium in the presence of the inhibitors; (c) the content of galactofuranose in the cell wall was significantly decreased in the presence of D-galactono-1,4-lactone. The influence on the mycelia growth was investigated by light microscopy (LM) and transmission electron microscopy (TEM) showing important alterations.","tracks":[{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":430,"end":437},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":456,"end":465},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A3","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":430,"end":437},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"},{"id":"T2","span":{"begin":456,"end":465},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T3","span":{"begin":456,"end":465},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":136},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":137,"end":356},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":357,"end":572},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":573,"end":1083},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":1084,"end":1235},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":136},"obj":"Sentence"},{"id":"T2","span":{"begin":137,"end":356},"obj":"Sentence"},{"id":"T3","span":{"begin":357,"end":572},"obj":"Sentence"},{"id":"T4","span":{"begin":573,"end":1083},"obj":"Sentence"},{"id":"T5","span":{"begin":1084,"end":1235},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":70,"end":92},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":195,"end":217},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"70095"},{"id":"A2","pred":"db_id","subj":"T2","obj":"70095"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1007,"end":1011},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000060"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"Glycosmos15-CL","denotations":[{"id":"T1","span":{"begin":114,"end":125},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000286"},{"subj":"T1","pred":"source","obj":"Glycosmos15-CL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#9693ec","default":true},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#aaec93"},{"id":"sentences","color":"#ec93c4"},{"id":"NCBITAXON","color":"#93deec"},{"id":"Anatomy-UBERON","color":"#ece093"},{"id":"Glycosmos15-CL","color":"#c693ec"}]}]}}