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PubMed:11956479 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 2023-2032 gene:1191 denotes clusterin
T1 2184-2204 disease:C0007134 denotes renal cell carcinoma
R1 T0 T1 associated_with clusterin,renal cell carcinoma

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
11956479-0#16#25#gene1191 736-745 gene1191 denotes clusterin
11956479-0#42#62#diseaseC0007134 982-1226 diseaseC0007134 denotes rvival assay in suspension. To investigate the in vivo effects of clusterin over expression on metastatic potentials each cell line was injected into the tail vein or renal subcapsule of nonobese diabetic, severe combined immunodeficient mice a
11956479-2#51#60#gene1191 316-325 gene1191 denotes clusterin
11956479-2#103#113#diseaseC0027627 368-378 diseaseC0027627 denotes metastasis
11956479-5#38#47#gene1191 1048-1057 gene1191 denotes clusterin
11956479-5#264#272#diseaseC0729233 1274-1282 diseaseC0729233 denotes thoracic
16#25#gene119142#62#diseaseC0007134 11956479-0#16#25#gene1191 11956479-0#42#62#diseaseC0007134 associated_with clusterin,"rvival assay in suspension. To investigate the in vivo effects of clusterin over expression on metastatic potentials each cell line was injected into the tail vein or renal subcapsule of nonobese diabetic, severe combined immunodeficient mice a"
51#60#gene1191103#113#diseaseC0027627 11956479-2#51#60#gene1191 11956479-2#103#113#diseaseC0027627 associated_with clusterin,metastasis
38#47#gene1191264#272#diseaseC0729233 11956479-5#38#47#gene1191 11956479-5#264#272#diseaseC0729233 associated_with clusterin,thoracic

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 115-545 OBJECTIVE denotes We recently reported a protective role of clusterin expression against apoptosis induced by a wide variety of stimuli in several human cancer models. In the current study we tested the hypothesis that clusterin over expression confers a benefit for the metastasis of renal cell carcinoma through the inhibition of apoptosis induced by the various obstacles the cancer cells may confront after detachment from their primary origin.
T2 569-1305 METHODS denotes We introduced clusterin complementary DNA into human renal cell carcinoma ACHN cells, which do not express detectable level of clusterin expression, and generated the clusterin over expressing cell line ACHN/CL and the control vector only transfected cell line ACHN/C. In vitro anti-cell death activity under anchorage independent conditions among ACHN sublines was examined by limiting dilution assay and cell survival assay in suspension. To investigate the in vivo effects of clusterin over expression on metastatic potentials each cell line was injected into the tail vein or renal subcapsule of nonobese diabetic, severe combined immunodeficient mice and the metastatic features in all abdominal and thoracic organs were evaluated.
T3 1315-1977 RESULTS denotes ACHN/CL showed significantly enhanced growth in limiting dilution cultures compared with ACHN/C. The analysis of cell survival in the floating assay also revealed that ACHN/CL had a powerful survival advantage in suspension compared with ACHN/C. Furthermore, ACHN/CL formed more than 5-fold as many metastatic nodules in the lung after intravenous injection than ACHN/C. Similarly more marked lung metastasis was observed after implanting ACHN/CL cells into the renal subcapsule than after implanting ACHN/C cells. In contrast, there were no significant differences among ACHN sublines in the growth rates in vitro and in vivo, cell motility or invasive ability.
T4 1991-2205 CONCLUSIONS denotes These findings suggest that, if clusterin is over expressed, it prolongs cell survival under unfavorable conditions in the metastatic process, resulting in the enhanced metastatic potential of renal cell carcinoma.