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PubMed:11914347 JSONTXT

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PMID_GLOBAL

Id Subject Object Predicate Lexical cue
T1 0-101 Sentence denotes Use of the Caulobacter crescentus genome sequence to develop a method for systematic genetic mapping.
T2 102-230 Sentence denotes The functional analysis of sequenced genomes will be facilitated by the development of tools for the rapid mapping of mutations.
T3 231-423 Sentence denotes We have developed a systematic approach to genetic mapping in Caulobacter crescentus that is based on bacteriophage-mediated transduction of strategically placed antibiotic resistance markers.
T4 424-571 Sentence denotes The genomic DNA sequence was used to identify sites distributed evenly around the chromosome at which plasmids could be nondisruptively integrated.
T5 572-687 Sentence denotes DNA fragments from these sites were amplified by PCR and cloned into a kanamycin-resistant (Kan(r)) suicide vector.
T6 688-787 Sentence denotes Delivery of these plasmids into C. crescentus resulted in integration via homologous recombination.
T7 788-876 Sentence denotes A set of 41 strains containing Kan(r) markers at 100-kb intervals was thereby generated.
T8 877-1008 Sentence denotes These strains serve as donors for generalized transduction using bacteriophage phiCr30, which can transduce at least 120 kb of DNA.
T9 1009-1163 Sentence denotes Transductants are selected with kanamycin and screened for loss of the mutant phenotype to assess linkage between the marker and the site of the mutation.
T10 1164-1283 Sentence denotes The dependence of cotransduction frequency on sequence distance was evaluated using several markers and mutant strains.
T11 1284-1435 Sentence denotes With these data as a standard, previously unmapped mutations were readily localized to DNA sequence intervals equivalent to less than 1% of the genome.
T12 1436-1542 Sentence denotes Candidate genes within the interval were then examined further by subcloning and complementation analysis.
T13 1543-1666 Sentence denotes Mutations resulting in sensitivity to ampicillin, in nutritional auxotrophies, or temperature-sensitive growth were mapped.
T14 1667-1816 Sentence denotes This approach to genetic mapping should be applicable to other bacteria with sequenced genomes for which generalized transducing phage are available.