PubMed:11909600 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11909600","sourcedb":"PubMed","sourceid":"11909600","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11909600","text":"Preparation of (1--\u003e4)-beta-D-xylooligosaccharides from an acid hydrolysate of cotton-seed xylan: suitability of cotton-seed xylan as a starting material for the preparation of (1--\u003e4)-beta-D-xylooligosaccharides.\nCotton-seed residual cake, which is a byproduct of the process of oil extraction from the seed, was delignified with sodium hypochlorite (1% available chlorine). Xylan was then prepared from the delignified wet material by alkali extraction with 15% sodium hydroxide. The cotton-seed xylan contained 64.7% xylose and 9.4% uronic acid. The xylan was hydrolyzed with 0.125 M sulfuric acid at 90 degrees C for 15 min. The resultant hydrolysis products were separated by gel-permeation chromatography on BioGel P-4 and Toyopearl HW-40F columns connected in series, with water as an eluate. Xylose and xylooligosaccharides with a degree of polymerization ranging from DP 2 to 15 were separated under such conditions, and each xylooligosaccharide-containing peak fraction afforded a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that cotton-seed xylan is suitable for the preparation of xylose and xylooligosaccharides.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":213},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":214,"end":375},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":376,"end":481},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":482,"end":548},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":549,"end":628},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":629,"end":799},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":800,"end":1056},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1057,"end":1169},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":213},"obj":"Sentence"},{"id":"T2","span":{"begin":214,"end":375},"obj":"Sentence"},{"id":"T3","span":{"begin":376,"end":481},"obj":"Sentence"},{"id":"T4","span":{"begin":482,"end":548},"obj":"Sentence"},{"id":"T5","span":{"begin":549,"end":628},"obj":"Sentence"},{"id":"T6","span":{"begin":629,"end":799},"obj":"Sentence"},{"id":"T7","span":{"begin":800,"end":1056},"obj":"Sentence"},{"id":"T8","span":{"begin":1057,"end":1169},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":998,"end":1002},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000560"},{"subj":"T1","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ecbc93","default":true},{"id":"CL-cell","color":"#a293ec"}]}]}}