PubMed:11819253 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11819253","sourcedb":"PubMed","sourceid":"11819253","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/11819253","text":"Serum deprivation enhances DNA synthesis of human hepatoma SMMC7721 cells.\nAIM:To determine the relationship between serum deprivation or serum levels and cell proliferation of human hepatoma SMMC-7721 cells.METHODS:Human hepatoma SMMC-7721 cells were grown in RPMI 1640 supplemented with 10% fetal calf serum (FCS)in 5% CO(2) incubator at 37\u0026mgr; for 24h, and culture media were replaced to serum-free or different serum FCS levels (2.5%, 5%, 10%, 20% and 25%). Six h,12h,18h and 24h after the culture,the cells were incorporated \u0026mgr;(3)H\u0026mgr;-TdR for 4h. At last \u0026mgr;(3)H\u0026mgr;-TdR incorpor-ation was detected with liquid scintillation counting.RESULTS:DNA synthesis of SMMC-7721 cells could be sharply stimulated by short-time (6h) serum deprivation (the cpm value of (3)H-TdR incorporation of cells in serum-free was 39.32-fold higher than cells in 25% serum),and the incorporation of (3)H-TdR was negatively related to the serum levels.Longer-time serum starvation (12h, 18h and 24h) also greatly stimulated DNA synthesis, although the cpm value of (3)H-TdR incroporation was less than that in 6h serum deprivation. Morphology of cells cultured in different serum levels also showed significant difference.CONCLUSION:Compared with other cell lines such as BEL7404 and Swiss 3T3,human hepatoma SMMC-7721 cells had different response to the serum deprivation.Short-time serum deprivation could greatly stimulate DNA synthesis of human hepatoma SMMC-7721 cells.Precautions must be given to the changes of serum levels for the detection of growth factors and drugs using SMMC-7721 cells as a model.","tracks":[{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":50,"end":58},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":183,"end":191},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":222,"end":230},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":1290,"end":1298},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":1439,"end":1447},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0007256"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0007256"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0007256"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0007256"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0007256"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T4","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T5","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PMID_GLOBAL","color":"#eca893","default":true}]}]}}