PubMed:11819247 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11819247","sourcedb":"PubMed","sourceid":"11819247","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/11819247","text":"Partial isolation and identification of hepatic stimulator substance mRNA extracted from human fetal liver.\nAIM:To partially isolate and identify hepatic stimulator substance mRNA from human fetal liver tissues.METHODS:The poly (A)mRNA was extracted from human fetal liver tissues of 4-5 month gestation, fractionated by size on sucrose gradient centrifugation, translated into protein from each fraction in vitro and then its products were tested for HSS activity.RESULTS:Twenty-two 500 total RNA was obtained from human fetal liver tissues and pooled.mRNA of 420 was yielded,processed by oligo(dT)-cellulose column chromatography, then was size-fractionated by ultracentrifution on a continuous sucrose density gradient (5%-25%), and separated into 18 fractions.Translated products of mRNA in fraction 8 and 9 could produce a two-fold increase in the incorporation of( 3)H-TdR into DNA of SMMC-7721 hepatoma cells and in a heat resistant and organ-specific way.CONCLUSION:The partially purified HSS mRNA was obtained and this would facilitate the cloning of HSS using expression vectors.","tracks":[{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":452,"end":455},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":901,"end":909},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":997,"end":1000},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":1060,"end":1063},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0009318"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0007256"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0009318"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0009318"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T4","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PMID_GLOBAL","color":"#ecca93","default":true}]}]}}