PubMed:11809883 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T1	0-10	protein	denotes	hMutSalpha
T2	47-57	protein	denotes	hMutLalpha
T3	62-71	protein	denotes	hMutLbeta
T4	110-120	protein	denotes	hMutSalpha
T5	125-135	protein	denotes	hMutLalpha
T6	161-171	protein	denotes	hMutSalpha
T7	177-187	protein	denotes	hMutLalpha
T8	192-201	protein	denotes	hMutLbeta
T9	328-336	protein	denotes	proteins
T10	338-348	protein	denotes	hMutSalpha
T11	423-433	protein	denotes	hMutSalpha
T12	501-511	protein	denotes	hMutSalpha
T13	524-534	protein	denotes	hMutSalpha
T14	579-589	protein	denotes	hMutSalpha
T15	612-622	protein	denotes	hMutLalpha
T16	627-636	protein	denotes	hMutLbeta
T17	803-813	protein	denotes	hMutLalpha
T18	818-827	protein	denotes	hMutLbeta
T19	902-912	protein	denotes	hMutLalpha

Id	Subject	Object	Predicate	Lexical cue
T1	0-10	protein	denotes	hMutSalpha
T2	47-57	protein	denotes	hMutLalpha
T3	62-71	protein	denotes	hMutLbeta
T4	110-120	protein	denotes	hMutSalpha
T5	125-135	protein	denotes	hMutLalpha
T6	161-171	protein	denotes	hMutSalpha
T7	177-187	protein	denotes	hMutLalpha
T8	192-201	protein	denotes	hMutLbeta
T9	338-348	protein	denotes	hMutSalpha
T10	423-433	protein	denotes	hMutSalpha
T11	501-511	protein	denotes	hMutSalpha
T12	524-534	protein	denotes	hMutSalpha
T13	579-589	protein	denotes	hMutSalpha
T14	612-622	protein	denotes	hMutLalpha
T15	627-636	protein	denotes	hMutLbeta
T16	803-813	protein	denotes	hMutLalpha
T17	818-827	protein	denotes	hMutLbeta
T18	902-912	protein	denotes	hMutLalpha

Id	Subject	Object	Predicate	Lexical cue
T1	0-79	Sentence	denotes	hMutSalpha forms an ATP-dependent complex with hMutLalpha and hMutLbeta on DNA.
T2	80-456	Sentence	denotes	The DNA binding properties of hMutSalpha and hMutLalpha and complex formation of hMutSalpha with hMutLalpha and hMutLbeta were investigated using binding experiments on magnetic bead-coupled DNA substrates with nuclear extracts as well as purified proteins. hMutSalpha binding to homoduplex DNA was disrupted by lower NaCl concentrations than hMutSalpha binding to a mismatch.
T3	457-667	Sentence	denotes	ATP markedly reduced the salt resistance of hMutSalpha binding but hMutSalpha still retained affinity for heteroduplexes. hMutSalpha formed a complex with hMutLalpha and hMutLbeta on DNA in the presence of ATP.
T4	668-731	Sentence	denotes	This complex only formed on 81mer and not 32mer DNA substrates.
T5	732-892	Sentence	denotes	Complex formation was enhanced by a mismatch in the DNA substrate, and hMutLalpha and hMutLbeta were shown to enter the complex at different ATP concentrations.
T6	893-1014	Sentence	denotes	Purified hMutLalpha showed an intrinsic affinity for DNA, with a preference for single-stranded over double-stranded DNA.