PubMed:11790884 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":33,"end":52},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":228,"end":237},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":272,"end":288},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":307,"end":311},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":327,"end":346},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":348,"end":352},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":364,"end":379},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":389,"end":395},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":430,"end":439},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":503,"end":507},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":614,"end":624},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":644,"end":650},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T13","span":{"begin":669,"end":678},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T14","span":{"begin":863,"end":877},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T15","span":{"begin":863,"end":867},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T16","span":{"begin":1186,"end":1195},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T17","span":{"begin":1225,"end":1231},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T18","span":{"begin":1349,"end":1358},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T19","span":{"begin":1383,"end":1392},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0005108"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0005550"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0005055"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0005055"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0005055"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0005055"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0005525"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0005801"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0005550"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"0005055"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"0005550"},{"id":"A12","pred":"mondo_id","subj":"T12","obj":"0005801"},{"id":"A13","pred":"mondo_id","subj":"T13","obj":"0005550"},{"id":"A14","pred":"mondo_id","subj":"T14","obj":"0005187"},{"id":"A15","pred":"mondo_id","subj":"T15","obj":"0005055"},{"id":"A16","pred":"mondo_id","subj":"T16","obj":"0005550"},{"id":"A17","pred":"mondo_id","subj":"T17","obj":"0005801"},{"id":"A18","pred":"mondo_id","subj":"T18","obj":"0005550"},{"id":"A19","pred":"mondo_id","subj":"T19","obj":"0005550"}],"text":"Potential cellular signatures of viral infections in human hematopoietic cells.\nExpression profiling of cellular genes was performed using a 10,000 cDNA human gene array in order to identify expression changes following chronic infection of human hematopoietic cells with Kaposi's Sarcoma-associated Virus (KSHV) also known as Human Herpesvirus 8 (HHV8) and Human T cell leukemia virus-1 (HTLV-1). We performed cell-free in vitro infection of primary bone marrow derived CD34+ cells using semi-purified HHV8 and a mature IL-2 dependent T cell line, KIT 225, using highly concentrated viral stocks prepared from an infectious molecular clone of HTLV-1. Thirty days post infection, mRNA was isolated from infected cultures and uninfected controls and submitted for microarray analysis. More than 400 genes were differentially expressed more than two-fold following HHV8 infection of primary bone marrow derived CD34+ cells. Of these 400, interferon regulatory factor 4 (IRF4), cyclin B2, TBP-associated factor, eukaryotic elongation factor and pim 2 were up-regulated more than 3.5 fold. In contrast, less than 100 genes were differentially expressed more than two-fold following chronic infection of a mature T cell line with HTLV-1. Of these, only cdc7 was up-regulated more than 3.5 fold. These data may provide insight into cellular signatures of infection useful for diagnosis of infection as well as potential targets for therapeutic intervention."}

{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":471,"end":475},"obj":"protein"},{"id":"T2","span":{"begin":521,"end":525},"obj":"protein"},{"id":"T3","span":{"begin":909,"end":913},"obj":"protein"},{"id":"T4","span":{"begin":936,"end":966},"obj":"protein"},{"id":"T5","span":{"begin":968,"end":972},"obj":"protein"},{"id":"T6","span":{"begin":975,"end":984},"obj":"protein"},{"id":"T7","span":{"begin":986,"end":1007},"obj":"protein"},{"id":"T8","span":{"begin":1009,"end":1037},"obj":"protein"},{"id":"T9","span":{"begin":1042,"end":1047},"obj":"protein"}],"text":"Potential cellular signatures of viral infections in human hematopoietic cells.\nExpression profiling of cellular genes was performed using a 10,000 cDNA human gene array in order to identify expression changes following chronic infection of human hematopoietic cells with Kaposi's Sarcoma-associated Virus (KSHV) also known as Human Herpesvirus 8 (HHV8) and Human T cell leukemia virus-1 (HTLV-1). We performed cell-free in vitro infection of primary bone marrow derived CD34+ cells using semi-purified HHV8 and a mature IL-2 dependent T cell line, KIT 225, using highly concentrated viral stocks prepared from an infectious molecular clone of HTLV-1. Thirty days post infection, mRNA was isolated from infected cultures and uninfected controls and submitted for microarray analysis. More than 400 genes were differentially expressed more than two-fold following HHV8 infection of primary bone marrow derived CD34+ cells. Of these 400, interferon regulatory factor 4 (IRF4), cyclin B2, TBP-associated factor, eukaryotic elongation factor and pim 2 were up-regulated more than 3.5 fold. In contrast, less than 100 genes were differentially expressed more than two-fold following chronic infection of a mature T cell line with HTLV-1. Of these, only cdc7 was up-regulated more than 3.5 fold. These data may provide insight into cellular signatures of infection useful for diagnosis of infection as well as potential targets for therapeutic intervention."}

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":471,"end":475},"obj":"protein"},{"id":"T2","span":{"begin":521,"end":525},"obj":"protein"},{"id":"T3","span":{"begin":909,"end":913},"obj":"protein"},{"id":"T4","span":{"begin":936,"end":966},"obj":"protein"},{"id":"T5","span":{"begin":968,"end":972},"obj":"protein"},{"id":"T6","span":{"begin":975,"end":984},"obj":"protein"},{"id":"T7","span":{"begin":1042,"end":1047},"obj":"protein"},{"id":"T8","span":{"begin":1248,"end":1252},"obj":"protein"}],"text":"Potential cellular signatures of viral infections in human hematopoietic cells.\nExpression profiling of cellular genes was performed using a 10,000 cDNA human gene array in order to identify expression changes following chronic infection of human hematopoietic cells with Kaposi's Sarcoma-associated Virus (KSHV) also known as Human Herpesvirus 8 (HHV8) and Human T cell leukemia virus-1 (HTLV-1). We performed cell-free in vitro infection of primary bone marrow derived CD34+ cells using semi-purified HHV8 and a mature IL-2 dependent T cell line, KIT 225, using highly concentrated viral stocks prepared from an infectious molecular clone of HTLV-1. Thirty days post infection, mRNA was isolated from infected cultures and uninfected controls and submitted for microarray analysis. More than 400 genes were differentially expressed more than two-fold following HHV8 infection of primary bone marrow derived CD34+ cells. Of these 400, interferon regulatory factor 4 (IRF4), cyclin B2, TBP-associated factor, eukaryotic elongation factor and pim 2 were up-regulated more than 3.5 fold. In contrast, less than 100 genes were differentially expressed more than two-fold following chronic infection of a mature T cell line with HTLV-1. Of these, only cdc7 was up-regulated more than 3.5 fold. These data may provide insight into cellular signatures of infection useful for diagnosis of infection as well as potential targets for therapeutic intervention."}