PubMed:11790164 JSONTXT

{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":8,"end":30},"obj":"protein"},{"id":"T2","span":{"begin":35,"end":53},"obj":"protein"},{"id":"T3","span":{"begin":400,"end":403},"obj":"protein"},{"id":"T4","span":{"begin":752,"end":779},"obj":"protein"},{"id":"T5","span":{"begin":781,"end":787},"obj":"protein"},{"id":"T6","span":{"begin":793,"end":802},"obj":"protein"},{"id":"T7","span":{"begin":942,"end":948},"obj":"protein"},{"id":"T8","span":{"begin":986,"end":995},"obj":"protein"},{"id":"T9","span":{"begin":1153,"end":1159},"obj":"protein"},{"id":"T10","span":{"begin":1163,"end":1172},"obj":"protein"},{"id":"T11","span":{"begin":1226,"end":1232},"obj":"protein"},{"id":"T12","span":{"begin":1370,"end":1376},"obj":"protein"},{"id":"T13","span":{"begin":1456,"end":1462},"obj":"protein"}],"text":"Soluble interleukin 2 receptor and interleukin 1alpha in toxic epidermal necrolysis: a comparative analysis of serum and blister fluid samples.\nBACKGROUND: Toxic epidermal necrolysis (TEN) is a rare but severe adverse drug disease, characterized by extensive skin and mucosal detachment with participation of different immunoinflammatory pathways, in particular with early participation of activated CD8+ T lymphocytes.\nOBJECTIVE: To further study the potential role of T lymphocytes in the early phase of keratinocyte necrosis.\nDESIGN: Prospective study.\nSETTING: University hospitals.\nPATIENTS: Thirteen patients with clinical and histopathologic criteria of TEN and 6 patients with second-degree burns.\nMAIN OUTCOME MEASURES: Measurement of soluble interleukin (IL) 2 receptor (sIL-2R) and IL-1alpha in serum samples and fluid of recent blisters.\nRESULTS: In the blister fluid of patients with TEN, we found significantly higher levels of sIL-2R than in patients with burns, whereas IL-1alpha levels were higher in the blister fluid of burned patients. No significant differences were found in serum samples of patients with TEN and burns, in either sIL-2R or IL-1alpha. In TEN we also found significantly higher levels of sIL-2R in the blister fluid compared with serum samples, pointing to a predominantly local production contrasting with the low concentration of sIL-2R in the blister fluid of burned patients.\nCONCLUSIONS: Our findings of elevated sIL-2R levels in blister fluid of patients with TEN are probably related to a local down-regulation of an immunologically mediated cytotoxic reaction and further support the involvement of activated T lymphocytes in the early blisters of TEN."}

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":8,"end":30},"obj":"protein"},{"id":"T2","span":{"begin":35,"end":53},"obj":"protein"},{"id":"T3","span":{"begin":400,"end":403},"obj":"protein"},{"id":"T4","span":{"begin":752,"end":779},"obj":"protein"},{"id":"T5","span":{"begin":781,"end":787},"obj":"protein"},{"id":"T6","span":{"begin":793,"end":802},"obj":"protein"},{"id":"T7","span":{"begin":942,"end":948},"obj":"protein"},{"id":"T8","span":{"begin":986,"end":995},"obj":"protein"},{"id":"T9","span":{"begin":1153,"end":1159},"obj":"protein"},{"id":"T10","span":{"begin":1163,"end":1172},"obj":"protein"},{"id":"T11","span":{"begin":1226,"end":1232},"obj":"protein"},{"id":"T12","span":{"begin":1370,"end":1376},"obj":"protein"},{"id":"T13","span":{"begin":1456,"end":1462},"obj":"protein"}],"text":"Soluble interleukin 2 receptor and interleukin 1alpha in toxic epidermal necrolysis: a comparative analysis of serum and blister fluid samples.\nBACKGROUND: Toxic epidermal necrolysis (TEN) is a rare but severe adverse drug disease, characterized by extensive skin and mucosal detachment with participation of different immunoinflammatory pathways, in particular with early participation of activated CD8+ T lymphocytes.\nOBJECTIVE: To further study the potential role of T lymphocytes in the early phase of keratinocyte necrosis.\nDESIGN: Prospective study.\nSETTING: University hospitals.\nPATIENTS: Thirteen patients with clinical and histopathologic criteria of TEN and 6 patients with second-degree burns.\nMAIN OUTCOME MEASURES: Measurement of soluble interleukin (IL) 2 receptor (sIL-2R) and IL-1alpha in serum samples and fluid of recent blisters.\nRESULTS: In the blister fluid of patients with TEN, we found significantly higher levels of sIL-2R than in patients with burns, whereas IL-1alpha levels were higher in the blister fluid of burned patients. No significant differences were found in serum samples of patients with TEN and burns, in either sIL-2R or IL-1alpha. In TEN we also found significantly higher levels of sIL-2R in the blister fluid compared with serum samples, pointing to a predominantly local production contrasting with the low concentration of sIL-2R in the blister fluid of burned patients.\nCONCLUSIONS: Our findings of elevated sIL-2R levels in blister fluid of patients with TEN are probably related to a local down-regulation of an immunologically mediated cytotoxic reaction and further support the involvement of activated T lymphocytes in the early blisters of TEN."}