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PubMed:11741957 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-64 Sentence denotes A lipid-regulated docking site on vinculin for protein kinase C.
T2 65-269 Sentence denotes During cell spreading, binding of actin-organizing proteins to acidic phospholipids and phosphorylation are important for localization and activity of these proteins at nascent cell-matrix adhesion sites.
T3 270-455 Sentence denotes Here, we report on a transient interaction between the lipid-dependent protein kinase Calpha and vinculin, an early component of these sites, during spreading of HeLa cells on collagen.
T4 456-622 Sentence denotes In vitro binding of protein kinase Calpha to vinculin tail was found dependent on free calcium and acidic phospholipids but independent of a functional kinase domain.
T5 623-709 Sentence denotes The interaction was enhanced by conditions that favor the oligomerization of vinculin.
T6 710-912 Sentence denotes Phosphorylation by protein kinase Calpha reached 1.5 mol of phosphate/mol of vinculin tail and required the C-terminal hydrophobic hairpin, a putative phosphatidylinositol 4,5-bisphosphate-binding site.
T7 913-1046 Sentence denotes Mass spectroscopy of peptides derived from in vitro phosphorylated vinculin tail identified phosphorylation of serines 1033 and 1045.
T8 1047-1190 Sentence denotes Inhibition of C-terminal phospholipid binding at the vinculin tail by mutagenesis or deletion reduced the rate of phosphorylation to < or =50%.
T9 1191-1494 Sentence denotes We suggest a possible mechanism whereby phospholipid-regulated conformational changes in vinculin may lead to exposure of a docking site for protein kinase Calpha and subsequent phosphorylation of vinculin and/or vinculin interaction partners, thereby affecting the formation of cell adhesion complexes.

FSU-PRGE

Id Subject Object Predicate Lexical cue
T1 34-42 protein denotes vinculin
T2 47-63 protein denotes protein kinase C
T3 99-104 protein denotes actin
T4 341-362 protein denotes protein kinase Calpha
T5 367-375 protein denotes vinculin
T6 446-454 protein denotes collagen
T7 476-497 protein denotes protein kinase Calpha
T8 501-509 protein denotes vinculin
T9 608-614 protein denotes kinase
T10 700-708 protein denotes vinculin
T11 729-750 protein denotes protein kinase Calpha
T12 787-795 protein denotes vinculin
T13 980-988 protein denotes vinculin
T14 1100-1108 protein denotes vinculin
T15 1280-1288 protein denotes vinculin
T16 1332-1353 protein denotes protein kinase Calpha
T17 1388-1396 protein denotes vinculin
T18 1404-1412 protein denotes vinculin

PIR-corpus2

Id Subject Object Predicate Lexical cue
T1 34-42 protein denotes vinculin
T2 47-63 protein denotes protein kinase C
T3 99-124 protein denotes actin-organizing proteins
T4 325-348 protein denotes lipid-dependent protein
T5 367-375 protein denotes vinculin
T6 446-454 protein denotes collagen
T7 476-490 protein denotes protein kinase
T8 501-509 protein denotes vinculin
T9 608-614 protein denotes kinase
T10 700-708 protein denotes vinculin
T11 729-750 protein denotes protein kinase Calpha
T12 787-795 protein denotes vinculin
T13 980-988 protein denotes vinculin
T14 1100-1108 protein denotes vinculin
T15 1280-1288 protein denotes vinculin
T16 1332-1353 protein denotes protein kinase Calpha
T17 1388-1396 protein denotes vinculin
T18 1404-1412 protein denotes vinculin

PIR-corpus1

Id Subject Object Predicate Lexical cue
T1 34-42 protein denotes vinculin
T2 47-63 protein denotes protein kinase C
T3 99-124 protein denotes actin-organizing proteins
T4 222-230 protein denotes proteins
T5 341-348 protein denotes protein
T6 367-375 protein denotes vinculin
T7 446-454 protein denotes collagen
T8 476-490 protein denotes protein kinase
T9 700-708 protein denotes vinculin
T10 729-750 protein denotes protein kinase Calpha
T11 1280-1288 protein denotes vinculin
T12 1332-1353 protein denotes protein kinase Calpha
T13 1388-1396 protein denotes vinculin

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 510-514 Body_part denotes tail http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164
T3 796-800 Body_part denotes tail http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164
T5 989-993 Body_part denotes tail http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164
T7 1109-1113 Body_part denotes tail http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164