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PubMed:11713368 JSONTXT

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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-152 Sentence denotes The regulation of interleukin-8 by hypoxia in human macrophages--a potential role in the pathogenesis of the acute respiratory distress syndrome (ARDS).
T2 153-164 Sentence denotes BACKGROUND:
T3 165-272 Sentence denotes The acute respiratory distress syndrome (ARDS) represents a form of severe acute inflammatory lung disease.
T4 273-490 Sentence denotes We have previously demonstrated significantly raised interleukin-8 (IL-8) levels in the lungs of at-risk patients that progress to ARDS, and identified the alveolar macrophage as an important source of this chemokine.
T5 491-658 Sentence denotes We wished to extend this study in a well-defined group of patients with major trauma, and to investigate potential mechanisms for rapid intrapulmonary IL-8 generation.
T6 659-681 Sentence denotes MATERIALS AND METHODS:
T7 682-800 Sentence denotes Patients with major trauma underwent bronchoalveolar lavage (BAL) and IL-8 levels were measured in BAL fluid by ELISA.
T8 801-888 Sentence denotes Human macrophages were derived from peripheral blood monocytes from healthy volunteers.
T9 889-975 Sentence denotes Rabbit alveolar macrophages were obtained from ex-vivo lavage of healthy rabbit lungs.
T10 976-1052 Sentence denotes Macrophages were culture under normoxic or hypoxic (PO2 26 mmHg) conditions.
T11 1053-1183 Sentence denotes IL-8 and other proinflammatory mediator expression was measured by ELISA, northern blotting or multi-probe RNase protection assay.
T12 1184-1192 Sentence denotes RESULTS:
T13 1193-1350 Sentence denotes In patients with major trauma, IL-8 levels were significantly higher in patients that progressed to ARDS compared to those that did not (n = 56, P = 0.0001).
T14 1351-1428 Sentence denotes High IL-8 levels negatively correlated with PaO2/FiO2 (r = -0.56, P < 0.001).
T15 1429-1562 Sentence denotes In human monocyte derived macrophages hypoxia rapidly upregulated IL-8 protein (within 2 hours) and mRNA expression (within 30 mins).
T16 1563-1635 Sentence denotes Acute hypoxia also increased rabbit alveolar macrophage IL-8 expression.
T17 1636-1711 Sentence denotes Hypoxia increased DNA binding activity of AP-1 and C/EBP but not NF-kappaB.
T18 1712-1822 Sentence denotes Hypoxia induced HIF-1 expression, but cobaltous ions and desferrioxamine did not mimic hypoxic IL-8 induction.
T19 1823-1919 Sentence denotes Hypoxia downregulated a range of other proinflammatory mediators, including MCP-1 and TNF-alpha.
T20 1920-2049 Sentence denotes Both the pattern of cytokine expression and transcription factor activation by hypoxia was different to that seen with endotoxin.
T21 2050-2062 Sentence denotes CONCLUSIONS:
T22 2063-2172 Sentence denotes Rapidly raised intrapulmonary IL-8 levels are associated with ARDS progression in patients with major trauma.
T23 2173-2343 Sentence denotes Acute hypoxia, a clinically relevant stimulus, rapidly and selectively upregulates IL-8 in macrophages associated with a novel pattern of transcription factor activation.
T24 2344-2504 Sentence denotes Acute hypoxia may represent one of potentially several proinflammatory stimuli responsible for rapid intrapulmonary IL-8 generation in patients at-risk of ARDS.
T1 0-152 Sentence denotes The regulation of interleukin-8 by hypoxia in human macrophages--a potential role in the pathogenesis of the acute respiratory distress syndrome (ARDS).
T2 153-164 Sentence denotes BACKGROUND:
T3 165-272 Sentence denotes The acute respiratory distress syndrome (ARDS) represents a form of severe acute inflammatory lung disease.
T4 273-490 Sentence denotes We have previously demonstrated significantly raised interleukin-8 (IL-8) levels in the lungs of at-risk patients that progress to ARDS, and identified the alveolar macrophage as an important source of this chemokine.
T5 491-658 Sentence denotes We wished to extend this study in a well-defined group of patients with major trauma, and to investigate potential mechanisms for rapid intrapulmonary IL-8 generation.
T6 659-681 Sentence denotes MATERIALS AND METHODS:
T7 682-800 Sentence denotes Patients with major trauma underwent bronchoalveolar lavage (BAL) and IL-8 levels were measured in BAL fluid by ELISA.
T8 801-888 Sentence denotes Human macrophages were derived from peripheral blood monocytes from healthy volunteers.
T9 889-975 Sentence denotes Rabbit alveolar macrophages were obtained from ex-vivo lavage of healthy rabbit lungs.
T10 976-1052 Sentence denotes Macrophages were culture under normoxic or hypoxic (PO2 26 mmHg) conditions.
T11 1053-1183 Sentence denotes IL-8 and other proinflammatory mediator expression was measured by ELISA, northern blotting or multi-probe RNase protection assay.
T12 1184-1192 Sentence denotes RESULTS:
T13 1193-1350 Sentence denotes In patients with major trauma, IL-8 levels were significantly higher in patients that progressed to ARDS compared to those that did not (n = 56, P = 0.0001).
T14 1351-1428 Sentence denotes High IL-8 levels negatively correlated with PaO2/FiO2 (r = -0.56, P < 0.001).
T15 1429-1562 Sentence denotes In human monocyte derived macrophages hypoxia rapidly upregulated IL-8 protein (within 2 hours) and mRNA expression (within 30 mins).
T16 1563-1635 Sentence denotes Acute hypoxia also increased rabbit alveolar macrophage IL-8 expression.
T17 1636-1711 Sentence denotes Hypoxia increased DNA binding activity of AP-1 and C/EBP but not NF-kappaB.
T18 1712-1822 Sentence denotes Hypoxia induced HIF-1 expression, but cobaltous ions and desferrioxamine did not mimic hypoxic IL-8 induction.
T19 1823-1919 Sentence denotes Hypoxia downregulated a range of other proinflammatory mediators, including MCP-1 and TNF-alpha.
T20 1920-2049 Sentence denotes Both the pattern of cytokine expression and transcription factor activation by hypoxia was different to that seen with endotoxin.
T21 2050-2062 Sentence denotes CONCLUSIONS:
T22 2063-2172 Sentence denotes Rapidly raised intrapulmonary IL-8 levels are associated with ARDS progression in patients with major trauma.
T23 2173-2343 Sentence denotes Acute hypoxia, a clinically relevant stimulus, rapidly and selectively upregulates IL-8 in macrophages associated with a novel pattern of transcription factor activation.
T24 2344-2504 Sentence denotes Acute hypoxia may represent one of potentially several proinflammatory stimuli responsible for rapid intrapulmonary IL-8 generation in patients at-risk of ARDS.

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 165-658 BACKGROUND denotes The acute respiratory distress syndrome (ARDS) represents a form of severe acute inflammatory lung disease. We have previously demonstrated significantly raised interleukin-8 (IL-8) levels in the lungs of at-risk patients that progress to ARDS, and identified the alveolar macrophage as an important source of this chemokine. We wished to extend this study in a well-defined group of patients with major trauma, and to investigate potential mechanisms for rapid intrapulmonary IL-8 generation.
T2 682-1183 METHODS denotes Patients with major trauma underwent bronchoalveolar lavage (BAL) and IL-8 levels were measured in BAL fluid by ELISA. Human macrophages were derived from peripheral blood monocytes from healthy volunteers. Rabbit alveolar macrophages were obtained from ex-vivo lavage of healthy rabbit lungs. Macrophages were culture under normoxic or hypoxic (PO2 26 mmHg) conditions. IL-8 and other proinflammatory mediator expression was measured by ELISA, northern blotting or multi-probe RNase protection assay.
T3 1193-2049 RESULTS denotes In patients with major trauma, IL-8 levels were significantly higher in patients that progressed to ARDS compared to those that did not (n = 56, P = 0.0001). High IL-8 levels negatively correlated with PaO2/FiO2 (r = -0.56, P < 0.001). In human monocyte derived macrophages hypoxia rapidly upregulated IL-8 protein (within 2 hours) and mRNA expression (within 30 mins). Acute hypoxia also increased rabbit alveolar macrophage IL-8 expression. Hypoxia increased DNA binding activity of AP-1 and C/EBP but not NF-kappaB. Hypoxia induced HIF-1 expression, but cobaltous ions and desferrioxamine did not mimic hypoxic IL-8 induction. Hypoxia downregulated a range of other proinflammatory mediators, including MCP-1 and TNF-alpha. Both the pattern of cytokine expression and transcription factor activation by hypoxia was different to that seen with endotoxin.
T4 2063-2504 CONCLUSIONS denotes Rapidly raised intrapulmonary IL-8 levels are associated with ARDS progression in patients with major trauma. Acute hypoxia, a clinically relevant stimulus, rapidly and selectively upregulates IL-8 in macrophages associated with a novel pattern of transcription factor activation. Acute hypoxia may represent one of potentially several proinflammatory stimuli responsible for rapid intrapulmonary IL-8 generation in patients at-risk of ARDS.

PMID_GLOBAL

Id Subject Object Predicate Lexical cue mondo_id
T1 109-144 DiseaseOrPhenotypicFeature denotes acute respiratory distress syndrome 0006502
T2 146-150 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130
T4 169-204 DiseaseOrPhenotypicFeature denotes acute respiratory distress syndrome 0006502
T5 206-210 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130
T7 259-271 DiseaseOrPhenotypicFeature denotes lung disease 0005275
T8 404-408 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130
T10 569-575 DiseaseOrPhenotypicFeature denotes trauma 0021178
T11 702-708 DiseaseOrPhenotypicFeature denotes trauma 0021178
T12 743-746 DiseaseOrPhenotypicFeature denotes BAL 0019460
T13 781-784 DiseaseOrPhenotypicFeature denotes BAL 0019460
T14 1216-1222 DiseaseOrPhenotypicFeature denotes trauma 0021178
T15 1293-1297 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130
T17 2125-2129 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130
T19 2165-2171 DiseaseOrPhenotypicFeature denotes trauma 0021178
T20 2499-2503 DiseaseOrPhenotypicFeature denotes ARDS 0006502|0100130

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
11713368-0#18#31#gene3576 18-31 gene3576 denotes interleukin-8
11713368-0#109#144#diseaseC0035222 109-144 diseaseC0035222 denotes acute respiratory distress syndrome
11713368-0#146#150#diseaseC0035222 146-150 diseaseC0035222 denotes ARDS
18#31#gene3576109#144#diseaseC0035222 11713368-0#18#31#gene3576 11713368-0#109#144#diseaseC0035222 associated_with interleukin-8,acute respiratory distress syndrome
18#31#gene3576146#150#diseaseC0035222 11713368-0#18#31#gene3576 11713368-0#146#150#diseaseC0035222 associated_with interleukin-8,ARDS

DisGeNET

Id Subject Object Predicate Lexical cue
T0 1224-1228 gene:3576 denotes IL-8
T1 1293-1297 disease:C0035222 denotes ARDS
R1 T0 T1 associated_with IL-8,ARDS