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PubMed:11606073 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 0-24 gene:3082 denotes Hepatocyte growth factor
T1 97-121 disease:C0279671 denotes squamous cervical cancer
T2 25-39 gene:3082 denotes scatter factor
T3 97-121 disease:C0279671 denotes squamous cervical cancer
R1 T0 T1 associated_with Hepatocyte growth factor,squamous cervical cancer
R2 T2 T3 associated_with scatter factor,squamous cervical cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
11606073-0#25#39#gene3082 1278-1481 gene3082 denotes ssenger RNA (mRNA) was detected in stromal cells, and c-met mRNA was detected in SKG-IIIa and Hela-S3. Hela-S3 that initially showed weak intercellular contact freely invaded the Matrigel-coated multipor
11606073-0#97#121#diseaseC0279671 2521-2565 diseaseC0279671 denotes stromal invasion of squamous cervical cancer
11606073-2#30#33#gene3082 341-344 gene3082 denotes HGF
11606073-2#30#33#gene3082 341-344 gene3082 denotes HGF
11606073-2#65#80#diseaseC0007847 376-391 diseaseC0007847 denotes cervical cancer
11606073-2#65#80#diseaseC0302592 376-391 diseaseC0302592 denotes cervical cancer
11606073-2#65#80#diseaseC4048328 376-391 diseaseC4048328 denotes cervical cancer
11606073-2#184#199#diseaseC0007847 495-510 diseaseC0007847 denotes cervical cancer
11606073-2#184#199#diseaseC0302592 495-510 diseaseC0302592 denotes cervical cancer
11606073-2#184#199#diseaseC4048328 495-510 diseaseC4048328 denotes cervical cancer
11606073-2#140#154#diseaseC0001418 451-465 diseaseC0001418 denotes adenocarcinoma
25#39#gene308297#121#diseaseC0279671 11606073-0#25#39#gene3082 11606073-0#97#121#diseaseC0279671 associated_with "ssenger RNA (mRNA) was detected in stromal cells, and c-met mRNA was detected in SKG-IIIa and Hela-S3. Hela-S3 that initially showed weak intercellular contact freely invaded the Matrigel-coated multipor",stromal invasion of squamous cervical cancer
30#33#gene308265#80#diseaseC0007847 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC0007847 associated_with HGF,cervical cancer
30#33#gene308265#80#diseaseC0302592 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC0302592 associated_with HGF,cervical cancer
30#33#gene308265#80#diseaseC4048328 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC4048328 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC0007847 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC0007847 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC0302592 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC0302592 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC4048328 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC4048328 associated_with HGF,cervical cancer
30#33#gene3082140#154#diseaseC0001418 11606073-2#30#33#gene3082 11606073-2#140#154#diseaseC0001418 associated_with HGF,adenocarcinoma
30#33#gene308265#80#diseaseC0007847 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC0007847 associated_with HGF,cervical cancer
30#33#gene308265#80#diseaseC0302592 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC0302592 associated_with HGF,cervical cancer
30#33#gene308265#80#diseaseC4048328 11606073-2#30#33#gene3082 11606073-2#65#80#diseaseC4048328 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC0007847 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC0007847 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC0302592 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC0302592 associated_with HGF,cervical cancer
30#33#gene3082184#199#diseaseC4048328 11606073-2#30#33#gene3082 11606073-2#184#199#diseaseC4048328 associated_with HGF,cervical cancer
30#33#gene3082140#154#diseaseC0001418 11606073-2#30#33#gene3082 11606073-2#140#154#diseaseC0001418 associated_with HGF,adenocarcinoma

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 134-301 OBJECTIVE denotes The purpose of this study was to examine the relationship of hepatocyte growth factor/scatter factor (HGF/SF) to cell motility and invasion in uterine cervical cancer.
T2 311-1259 METHODS denotes We examined the expression of HGF/SF and its receptor, c-met, in cervical cancer cell lines SKG-IIIa (squamous cell carcinoma) and Hela-S3 (adenocarcinoma) and in stromal cells of the cervical cancer tissue by reverse transcription-polymerase chain reaction. We studied the effect of HGF/SF on invasiveness of SKG-IIIa and Hela-S3 in an invasion model of the modified Boyden chamber method and by electron microscopy. SKG-IIIa cells were also seeded on the thick Matrigel-coated layer to evaluate the invasion patterns in three-dimensional directions. To investigate the mechanism of an inductive effect of HGF/SF on the invasiveness of SKG-IIIa, we examined the effect of HGF/SF on the expression of intercellular adhesion molecule E-cadherin, cell-substrate adhesion molecules CD44, alpha2beta1, and alpha6beta1, and intracellular skeleton fiber actin in SKG-IIIa in cell enzyme-linked immunosorbent assay (ELISA) and immunofluorescence staining.
T3 1269-2428 RESULTS denotes HGF/SF messenger RNA (mRNA) was detected in stromal cells, and c-met mRNA was detected in SKG-IIIa and Hela-S3. Hela-S3 that initially showed weak intercellular contact freely invaded the Matrigel-coated multiporous membrane without the addition of HGF/SF. In contrast, SKG-IIIa that initially showed strong intercellular adhesion could invade the membrane after the addition of HGF/SF. The same results were represented by an addition of HECD-1, an anti-human E-cadherin antibody. In an experiment with cell culture in a thick Matrigel layer, control SKG-IIIa showed a mirror-ball-like invasion pattern, whereas HGF/SF-stimulated SKG-IIIa spread horizontally over the membrane and migrated through the membrane holes, presenting a tentacular invasion pattern. Migration of SKG-IIIa under the membrane was confirmed by scanning and transmission electron microscopy. The addition of HGF/SF in cell ELISA assay decreased the expression of E-cadherin and actin in SKG-IIIa, but it did not change the expression of CD44, alpha2beta1, and alpha6beta1. Immunofluorescence staining revealed that the expression of E-cadherin in cell membrane was disturbed by HGF/SF.
T4 2442-2636 CONCLUSIONS denotes Our data indicate that HGF/SF produced by stromal cells influences the mode of stromal invasion of squamous cervical cancer by selectively decreasing the expression of both E-cadherin and actin.