| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-125 |
Sentence |
denotes |
Dbp9p, a putative ATP-dependent RNA helicase involved in 60S-ribosomal-subunit biogenesis, functionally interacts with Dbp6p. |
| T2 |
126-215 |
Sentence |
denotes |
Ribosome synthesis is a highly complex process and constitutes a major cellular activity. |
| T3 |
216-419 |
Sentence |
denotes |
The biogenesis of this ribonucleoprotein assembly requires a multitude of protein trans-acting factors including several putative ATP-dependent RNA helicases of the DEAD-box and related protein families. |
| T4 |
420-649 |
Sentence |
denotes |
Here we show that the previously uncharacterized Saccharomyces cerevisiae open reading frame YLR276C, hereafter named DBP9 (DEAD-box protein 9), encodes an essential nucleolar protein involved in 60S-ribosomal-subunit biogenesis. |
| T5 |
650-765 |
Sentence |
denotes |
Genetic depletion of Dbp9p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. |
| T6 |
766-980 |
Sentence |
denotes |
This terminal phenotype is likely due to the instability of early pre-ribosomal particles, as evidenced by the low steady-state levels and the decreased synthesis of the 27S precursors to mature 25S and 5.8S rRNAs. |
| T7 |
981-1180 |
Sentence |
denotes |
In agreement with a role of Dbp9p in 60S subunit synthesis, we find that increased Dbp9p dosage efficiently suppresses certain dbp6 alleles and that dbp6/dbp9 double mutants show synthetic lethality. |
| T8 |
1181-1254 |
Sentence |
denotes |
Furthermore, Dbp6p and Dbp9p weakly interact in a yeast two-hybrid assay. |
| T9 |
1255-1401 |
Sentence |
denotes |
Altogether, our findings indicate an intimate functional interaction between Dbp6p and Dbp9p during the process of 60S-ribosomal-subunit assembly. |