PubMed:11546873 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11546873","sourcedb":"PubMed","sourceid":"11546873","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11546873","text":"Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex.\nNonsense-mediated messenger RNA (mRNA) decay, or NMD, is a critical process of selective degradation of mRNAs that contain premature stop codons. NMD depends on both pre-mRNA splicing and translation, and it requires recognition of the position of stop codons relative to exon-exon junctions. A key factor in NMD is hUpf3, a mostly nuclear protein that shuttles between the nucleus and cytoplasm and interacts specifically with spliced mRNAs. We found that hUpf3 interacts with Y14, a component of post-splicing mRNA-protein (mRNP) complexes, and that hUpf3 is enriched in Y14-containing mRNP complexes. The mRNA export factors Aly/REF and TAP are also associated with nuclear hUpf3, indicating that hUpf3 is in mRNP complexes that are poised for nuclear export. Like Y14 and Aly/REF, hUpf3 binds to spliced mRNAs specifically ( approximately 20 nucleotides) upstream of exon-exon junctions. The splicing-dependent binding of hUpf3 to mRNAs before export, as part of the complex that assembles near exon-exon junctions, allows it to serve as a link between splicing and NMD in the cytoplasm.","tracks":[{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":43,"end":48},"obj":"protein"},{"id":"T2","span":{"begin":419,"end":424},"obj":"protein"},{"id":"T3","span":{"begin":560,"end":565},"obj":"protein"},{"id":"T4","span":{"begin":581,"end":584},"obj":"protein"},{"id":"T5","span":{"begin":655,"end":660},"obj":"protein"},{"id":"T6","span":{"begin":676,"end":679},"obj":"protein"},{"id":"T7","span":{"begin":731,"end":738},"obj":"protein"},{"id":"T8","span":{"begin":743,"end":746},"obj":"protein"},{"id":"T9","span":{"begin":780,"end":785},"obj":"protein"},{"id":"T10","span":{"begin":803,"end":808},"obj":"protein"},{"id":"T11","span":{"begin":871,"end":874},"obj":"protein"},{"id":"T12","span":{"begin":879,"end":886},"obj":"protein"},{"id":"T13","span":{"begin":888,"end":893},"obj":"protein"},{"id":"T14","span":{"begin":1029,"end":1034},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"FSU-PRGE"},{"subj":"T2","pred":"source","obj":"FSU-PRGE"},{"subj":"T3","pred":"source","obj":"FSU-PRGE"},{"subj":"T4","pred":"source","obj":"FSU-PRGE"},{"subj":"T5","pred":"source","obj":"FSU-PRGE"},{"subj":"T6","pred":"source","obj":"FSU-PRGE"},{"subj":"T7","pred":"source","obj":"FSU-PRGE"},{"subj":"T8","pred":"source","obj":"FSU-PRGE"},{"subj":"T9","pred":"source","obj":"FSU-PRGE"},{"subj":"T10","pred":"source","obj":"FSU-PRGE"},{"subj":"T11","pred":"source","obj":"FSU-PRGE"},{"subj":"T12","pred":"source","obj":"FSU-PRGE"},{"subj":"T13","pred":"source","obj":"FSU-PRGE"},{"subj":"T14","pred":"source","obj":"FSU-PRGE"}]},{"project":"PIR-corpus2","denotations":[{"id":"T1","span":{"begin":12,"end":48},"obj":"protein"},{"id":"T2","span":{"begin":419,"end":424},"obj":"protein"},{"id":"T3","span":{"begin":435,"end":450},"obj":"protein"},{"id":"T4","span":{"begin":560,"end":565},"obj":"protein"},{"id":"T5","span":{"begin":588,"end":600},"obj":"protein"},{"id":"T6","span":{"begin":655,"end":660},"obj":"protein"},{"id":"T7","span":{"begin":676,"end":705},"obj":"protein"},{"id":"T8","span":{"begin":711,"end":738},"obj":"protein"},{"id":"T9","span":{"begin":743,"end":746},"obj":"protein"},{"id":"T10","span":{"begin":772,"end":785},"obj":"protein"},{"id":"T11","span":{"begin":803,"end":808},"obj":"protein"},{"id":"T12","span":{"begin":815,"end":819},"obj":"protein"},{"id":"T13","span":{"begin":879,"end":886},"obj":"protein"},{"id":"T14","span":{"begin":888,"end":893},"obj":"protein"},{"id":"T15","span":{"begin":1029,"end":1034},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"PIR-corpus2"},{"subj":"T2","pred":"source","obj":"PIR-corpus2"},{"subj":"T3","pred":"source","obj":"PIR-corpus2"},{"subj":"T4","pred":"source","obj":"PIR-corpus2"},{"subj":"T5","pred":"source","obj":"PIR-corpus2"},{"subj":"T6","pred":"source","obj":"PIR-corpus2"},{"subj":"T7","pred":"source","obj":"PIR-corpus2"},{"subj":"T8","pred":"source","obj":"PIR-corpus2"},{"subj":"T9","pred":"source","obj":"PIR-corpus2"},{"subj":"T10","pred":"source","obj":"PIR-corpus2"},{"subj":"T11","pred":"source","obj":"PIR-corpus2"},{"subj":"T12","pred":"source","obj":"PIR-corpus2"},{"subj":"T13","pred":"source","obj":"PIR-corpus2"},{"subj":"T14","pred":"source","obj":"PIR-corpus2"},{"subj":"T15","pred":"source","obj":"PIR-corpus2"}]},{"project":"PIR-corpus1","denotations":[{"id":"T1","span":{"begin":43,"end":48},"obj":"protein"},{"id":"T2","span":{"begin":419,"end":424},"obj":"protein"},{"id":"T3","span":{"begin":443,"end":450},"obj":"protein"},{"id":"T4","span":{"begin":560,"end":565},"obj":"protein"},{"id":"T5","span":{"begin":581,"end":584},"obj":"protein"},{"id":"T6","span":{"begin":588,"end":600},"obj":"protein"},{"id":"T7","span":{"begin":655,"end":660},"obj":"protein"},{"id":"T8","span":{"begin":691,"end":705},"obj":"protein"},{"id":"T9","span":{"begin":716,"end":738},"obj":"protein"},{"id":"T10","span":{"begin":780,"end":785},"obj":"protein"},{"id":"T11","span":{"begin":803,"end":808},"obj":"protein"},{"id":"T12","span":{"begin":815,"end":819},"obj":"protein"},{"id":"T13","span":{"begin":871,"end":874},"obj":"protein"},{"id":"T14","span":{"begin":879,"end":886},"obj":"protein"},{"id":"T15","span":{"begin":888,"end":893},"obj":"protein"},{"id":"T16","span":{"begin":1029,"end":1034},"obj":"protein"},{"id":"T17","span":{"begin":1074,"end":1081},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"PIR-corpus1"},{"subj":"T2","pred":"source","obj":"PIR-corpus1"},{"subj":"T3","pred":"source","obj":"PIR-corpus1"},{"subj":"T4","pred":"source","obj":"PIR-corpus1"},{"subj":"T5","pred":"source","obj":"PIR-corpus1"},{"subj":"T6","pred":"source","obj":"PIR-corpus1"},{"subj":"T7","pred":"source","obj":"PIR-corpus1"},{"subj":"T8","pred":"source","obj":"PIR-corpus1"},{"subj":"T9","pred":"source","obj":"PIR-corpus1"},{"subj":"T10","pred":"source","obj":"PIR-corpus1"},{"subj":"T11","pred":"source","obj":"PIR-corpus1"},{"subj":"T12","pred":"source","obj":"PIR-corpus1"},{"subj":"T13","pred":"source","obj":"PIR-corpus1"},{"subj":"T14","pred":"source","obj":"PIR-corpus1"},{"subj":"T15","pred":"source","obj":"PIR-corpus1"},{"subj":"T16","pred":"source","obj":"PIR-corpus1"},{"subj":"T17","pred":"source","obj":"PIR-corpus1"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"FSU-PRGE","color":"#96ec93","default":true},{"id":"PIR-corpus2","color":"#ec93b0"},{"id":"PIR-corpus1","color":"#93caec"}]}]}}