PubMed:11483512 JSONTXT

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    ggdb-test

    {"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":10,"end":15},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T2","span":{"begin":86,"end":90},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T3","span":{"begin":95,"end":99},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T4","span":{"begin":558,"end":562},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T5","span":{"begin":567,"end":571},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T6","span":{"begin":634,"end":639},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T7","span":{"begin":674,"end":679},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T8","span":{"begin":763,"end":768},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T9","span":{"begin":923,"end":928},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T10","span":{"begin":957,"end":961},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T11","span":{"begin":966,"end":970},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T12","span":{"begin":981,"end":986},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T13","span":{"begin":1042,"end":1046},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T14","span":{"begin":1051,"end":1055},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T15","span":{"begin":1139,"end":1144},"obj":"https://acgg.asia/db/ggdb/info/gg233"}],"text":"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.\nMany eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively."}

    PMID_GLOBAL

    {"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":0,"end":100},"obj":"Sentence"},{"id":"T2","span":{"begin":101,"end":214},"obj":"Sentence"},{"id":"T3","span":{"begin":215,"end":380},"obj":"Sentence"},{"id":"T4","span":{"begin":381,"end":494},"obj":"Sentence"},{"id":"T5","span":{"begin":495,"end":572},"obj":"Sentence"},{"id":"T6","span":{"begin":573,"end":640},"obj":"Sentence"},{"id":"T7","span":{"begin":641,"end":752},"obj":"Sentence"},{"id":"T8","span":{"begin":753,"end":887},"obj":"Sentence"},{"id":"T9","span":{"begin":888,"end":1056},"obj":"Sentence"},{"id":"T10","span":{"begin":1057,"end":1169},"obj":"Sentence"}],"text":"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.\nMany eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively."}

    GGDB-2020

    {"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":10,"end":15},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T2","span":{"begin":86,"end":90},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T3","span":{"begin":95,"end":99},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T4","span":{"begin":558,"end":562},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T5","span":{"begin":567,"end":571},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T6","span":{"begin":634,"end":639},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T7","span":{"begin":674,"end":679},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T8","span":{"begin":763,"end":768},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T9","span":{"begin":923,"end":928},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T10","span":{"begin":957,"end":961},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T11","span":{"begin":966,"end":970},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T12","span":{"begin":981,"end":986},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"T13","span":{"begin":1042,"end":1046},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"T14","span":{"begin":1051,"end":1055},"obj":"https://acgg.asia/db/ggdb/info/gg230"},{"id":"T15","span":{"begin":1139,"end":1144},"obj":"https://acgg.asia/db/ggdb/info/gg233"}],"text":"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.\nMany eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively."}

    bionlp-st-pc-2013-training

    {"project":"bionlp-st-pc-2013-training","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"Gene_or_gene_product"},{"id":"T2","span":{"begin":10,"end":15},"obj":"Gene_or_gene_product"},{"id":"T3","span":{"begin":31,"end":34},"obj":"Simple_chemical"},{"id":"T4","span":{"begin":86,"end":90},"obj":"Gene_or_gene_product"},{"id":"T5","span":{"begin":95,"end":99},"obj":"Gene_or_gene_product"},{"id":"T6","span":{"begin":117,"end":129},"obj":"Cellular_component"},{"id":"T7","span":{"begin":159,"end":174},"obj":"Cellular_component"},{"id":"T8","span":{"begin":179,"end":207},"obj":"Simple_chemical"},{"id":"T9","span":{"begin":209,"end":212},"obj":"Simple_chemical"},{"id":"T10","span":{"begin":219,"end":235},"obj":"Complex"},{"id":"T11","span":{"begin":245,"end":248},"obj":"Simple_chemical"},{"id":"T12","span":{"begin":266,"end":287},"obj":"Cellular_component"},{"id":"T13","span":{"begin":325,"end":328},"obj":"Simple_chemical"},{"id":"T14","span":{"begin":376,"end":379},"obj":"Simple_chemical"},{"id":"T15","span":{"begin":422,"end":438},"obj":"Complex"},{"id":"T16","span":{"begin":447,"end":455},"obj":"Simple_chemical"},{"id":"T17","span":{"begin":517,"end":533},"obj":"Complex"},{"id":"T18","span":{"begin":558,"end":562},"obj":"Gene_or_gene_product"},{"id":"T19","span":{"begin":567,"end":571},"obj":"Gene_or_gene_product"},{"id":"T20","span":{"begin":624,"end":629},"obj":"Gene_or_gene_product"},{"id":"T21","span":{"begin":634,"end":639},"obj":"Gene_or_gene_product"},{"id":"T22","span":{"begin":664,"end":669},"obj":"Gene_or_gene_product"},{"id":"T23","span":{"begin":674,"end":679},"obj":"Gene_or_gene_product"},{"id":"T24","span":{"begin":753,"end":758},"obj":"Gene_or_gene_product"},{"id":"T25","span":{"begin":763,"end":768},"obj":"Gene_or_gene_product"},{"id":"T26","span":{"begin":814,"end":817},"obj":"Simple_chemical"},{"id":"T27","span":{"begin":864,"end":872},"obj":"Simple_chemical"},{"id":"T28","span":{"begin":913,"end":918},"obj":"Gene_or_gene_product"},{"id":"T29","span":{"begin":923,"end":928},"obj":"Gene_or_gene_product"},{"id":"T30","span":{"begin":957,"end":961},"obj":"Gene_or_gene_product"},{"id":"T31","span":{"begin":966,"end":970},"obj":"Gene_or_gene_product"},{"id":"T32","span":{"begin":981,"end":986},"obj":"Gene_or_gene_product"},{"id":"T33","span":{"begin":1042,"end":1046},"obj":"Gene_or_gene_product"},{"id":"T34","span":{"begin":1051,"end":1055},"obj":"Gene_or_gene_product"},{"id":"T35","span":{"begin":1082,"end":1088},"obj":"Gene_or_gene_product"},{"id":"T36","span":{"begin":1090,"end":1097},"obj":"Gene_or_gene_product"},{"id":"T37","span":{"begin":1103,"end":1109},"obj":"Gene_or_gene_product"},{"id":"T38","span":{"begin":1111,"end":1118},"obj":"Gene_or_gene_product"},{"id":"T39","span":{"begin":1139,"end":1144},"obj":"Gene_or_gene_product"},{"id":"T40","span":{"begin":1149,"end":1154},"obj":"Gene_or_gene_product"}],"text":"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.\nMany eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively."}

    glycogenes

    {"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":0,"end":3},"obj":"https://acgg.asia/db/ggdb/info/gg232"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":10,"end":15},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":86,"end":90},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":558,"end":562},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":624,"end":627},"obj":"https://acgg.asia/db/ggdb/info/gg232"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":634,"end":639},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":664,"end":667},"obj":"https://acgg.asia/db/ggdb/info/gg232"},{"id":"PD-GlycoGenes20190927-B_T8","span":{"begin":674,"end":679},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T9","span":{"begin":753,"end":756},"obj":"https://acgg.asia/db/ggdb/info/gg232"},{"id":"PD-GlycoGenes20190927-B_T10","span":{"begin":763,"end":768},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T11","span":{"begin":913,"end":916},"obj":"https://acgg.asia/db/ggdb/info/gg232"},{"id":"PD-GlycoGenes20190927-B_T12","span":{"begin":923,"end":928},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T13","span":{"begin":957,"end":961},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"PD-GlycoGenes20190927-B_T14","span":{"begin":981,"end":986},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T15","span":{"begin":1042,"end":1046},"obj":"https://acgg.asia/db/ggdb/info/gg231"},{"id":"PD-GlycoGenes20190927-B_T16","span":{"begin":1139,"end":1144},"obj":"https://acgg.asia/db/ggdb/info/gg233"},{"id":"PD-GlycoGenes20190927-B_T17","span":{"begin":1149,"end":1152},"obj":"https://acgg.asia/db/ggdb/info/gg232"}],"text":"PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.\nMany eukaryotic cell surface proteins are anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). The GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with a substrate protein. It was known that the GPI transamidase is a complex containing GAA1 and GPI8. Here, we report two new components of this enzyme: PIG-S and PIG-T. To determine roles for PIG-S and PIG-T, we disrupted these genes in mouse F9 cells by homologous recombination. PIG-S and PIG-T knockout cells were defective in transfer of GPI to proteins, particularly in formation of the carbonyl intermediates. We also demonstrate that PIG-S and PIG-T form a protein complex with GAA1 and GPI8, and that PIG-T maintains the complex by stabilizing the expression of GAA1 and GPI8. Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively."}