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PubMed:11479276 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 653-660 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T2 783-790 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T3 1186-1193 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T4 1425-1432 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T5 1617-1624 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 653-660 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T2 783-790 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T3 1186-1193 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T4 1425-1432 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T5 1617-1624 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 653-660 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T2 783-790 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T3 1186-1193 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T4 1425-1432 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T5 1617-1624 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 55-70 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC228,LEC233,LEC239,LEC279,LEC342,LEC505 denotes Vibrio cholerae
_T2 426-441 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC228,LEC233,LEC239,LEC279,LEC342,LEC505 denotes Vibrio cholerae
_T3 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T4 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T7 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T8 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T9 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli
_T10 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T11 1011-1027 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli

ICD10

Id Subject Object Predicate Lexical cue
T1 62-70 http://purl.bioontology.org/ontology/ICD10/A00.9 denotes cholerae
T2 433-441 http://purl.bioontology.org/ontology/ICD10/A00.9 denotes cholerae
T3 996-1004 http://purl.bioontology.org/ontology/ICD10/A00.9 denotes cholerae
T4 62-70 http://purl.bioontology.org/ontology/ICD10/A00 denotes cholerae
T5 433-441 http://purl.bioontology.org/ontology/ICD10/A00 denotes cholerae
T6 996-1004 http://purl.bioontology.org/ontology/ICD10/A00 denotes cholerae

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 42-49 FMAID:165447 denotes protein
_T2 42-49 FMAID:67257 denotes protein
_T3 155-169 FMAID:82741 denotes monosaccharide
_T4 155-169 FMAID:196730 denotes monosaccharide
_T5 282-301 FMAID:196779 denotes lipopolysaccharides
_T6 282-301 FMAID:82785 denotes lipopolysaccharides
_T7 282-301 FMAID:82746 denotes lipopolysaccharides
_T8 282-301 FMAID:196735 denotes lipopolysaccharides
_T9 640-652 FMAID:179268 denotes intermediate
_T10 640-652 FMAID:74531 denotes intermediate
_T11 653-660 FMAID:82801 denotes mannose
_T12 653-660 FMAID:196796 denotes mannose
_T13 783-790 FMAID:196796 denotes mannose
_T14 783-790 FMAID:82801 denotes mannose
_T15 984-989 FMAID:198663 denotes genes
_T16 1186-1193 FMAID:82801 denotes mannose
_T17 1186-1193 FMAID:196796 denotes mannose
_T18 1425-1432 FMAID:196796 denotes mannose
_T19 1425-1432 FMAID:82801 denotes mannose
_T20 1511-1518 FMAID:67257 denotes protein
_T21 1511-1518 FMAID:165447 denotes protein
_T22 1567-1577 FMAID:166112 denotes mg protein
_T23 1570-1577 FMAID:165447 denotes protein
_T24 1570-1577 FMAID:67257 denotes protein
_T25 1617-1624 FMAID:196796 denotes mannose
_T26 1617-1624 FMAID:82801 denotes mannose
_T27 1705-1715 FMAID:166112 denotes mg protein
_T28 1708-1715 FMAID:165447 denotes protein
_T29 1708-1715 FMAID:67257 denotes protein
_T30 1871-1880 FMAID:66644 denotes system in
_T31 1871-1880 FMAID:185472 denotes system in

uniprot-human

Id Subject Object Predicate Lexical cue
T1 535-559 http://www.uniprot.org/uniprot/P34949 denotes phosphomannose isomerase
T2 745-752 http://www.uniprot.org/uniprot/O15305 denotes 5.4.2.8
T3 745-752 http://www.uniprot.org/uniprot/Q92871 denotes 5.4.2.8
T4 777-790 http://www.uniprot.org/uniprot/O60547 denotes GDP-D-mannose
T5 854-862 http://www.uniprot.org/uniprot/O60547 denotes 4.2.1.47

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 535-559 http://www.uniprot.org/uniprot/Q924M7 denotes phosphomannose isomerase
T2 745-752 http://www.uniprot.org/uniprot/O35621 denotes 5.4.2.8
T3 745-752 http://www.uniprot.org/uniprot/Q9Z2M7 denotes 5.4.2.8
T4 854-862 http://www.uniprot.org/uniprot/Q8K0C9 denotes 4.2.1.47

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 55-70 http://purl.bioontology.org/ontology/NCBITAXON/666 denotes Vibrio cholerae
T2 55-70 http://purl.bioontology.org/ontology/NCBITAXON/650003 denotes Vibrio cholerae
T3 329-351 http://purl.bioontology.org/ontology/NCBITAXON/130805 denotes Gram-negative bacteria
T4 329-351 http://purl.bioontology.org/ontology/NCBITAXON/130804 denotes Gram-negative bacteria
T5 343-351 http://purl.bioontology.org/ontology/NCBITAXON/2 denotes bacteria
T6 343-351 http://purl.bioontology.org/ontology/NCBITAXON/629395 denotes bacteria
T7 426-441 http://purl.bioontology.org/ontology/NCBITAXON/666 denotes Vibrio cholerae
T8 426-441 http://purl.bioontology.org/ontology/NCBITAXON/650003 denotes Vibrio cholerae
T9 1011-1022 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-134 Sentence denotes Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine.
TextSentencer_T2 135-352 Sentence denotes The 4-amino-6-deoxy-monosaccharide D-perosamine is an important element in the glycosylation of interesting cell products, such as antibiotics and lipopolysaccharides (LPS) of Gram-positive and Gram-negative bacteria.
TextSentencer_T3 353-616 Sentence denotes The biosynthetic pathway of the precursor molecule, GDP-D-perosamine, in Vibrio cholerae O1 starts with an isomerisation of fructose-6-phosphate catalyzed by the bifunctional enzyme phosphomannose isomerase-guanosine diphosphomannose pyrophosphorylase (RfbA; E.C.
TextSentencer_T4 617-744 Sentence denotes 2.7.7.22) creating the intermediate mannose-6-phosphate, which is subsequently converted by the phosphomanno-mutase (RfbB; E.C.
TextSentencer_T5 745-853 Sentence denotes 5.4.2.8) and further by RfbA to GDP-D-mannose, to GDP-4 keto-6-deoxymannose by a 4,6-dehydratase (RfbD; E.C.
TextSentencer_T6 854-951 Sentence denotes 4.2.1.47) and finally to GDP-D-perosamine by an aminotransferase (RfbE; E.C. not yet classified).
TextSentencer_T7 952-1047 Sentence denotes We cloned the rfbD and the rfbE genes of V. cholerae O1 in Escherichia coli expression vectors.
TextSentencer_T8 1048-1149 Sentence denotes Both biosynthetic enzymes were overproduced in E. coli BL21 (DE3) and their activities were analyzed.
TextSentencer_T9 1150-1366 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-D-perosamine was optimized and the final product, GDP-D-perosamine, was purified and identified by nuclear magnetic resonance, mass spectrometry, and chromatography.
TextSentencer_T10 1367-1486 Sentence denotes The catalytically active form of the GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase seems to be a tetramer of 170 kDa.
TextSentencer_T11 1487-1625 Sentence denotes The His-tag RfbE fusion protein has a Km of 0.06 mM and a Vmax value of 38 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose.
TextSentencer_T12 1626-1730 Sentence denotes The Km and Vmax values for the cosubstrate L-glutamate were 0.1 mM and 42 nkat/mg protein, respectively.
TextSentencer_T13 1731-1927 Sentence denotes The intention of this work is to establish a basis for both the in vitro production of GDP-D-perosamine and for an in vivo perosaminylation system in a suitable bacterial host, preferably E. coli.
T1 0-134 Sentence denotes Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine.
T2 135-352 Sentence denotes The 4-amino-6-deoxy-monosaccharide D-perosamine is an important element in the glycosylation of interesting cell products, such as antibiotics and lipopolysaccharides (LPS) of Gram-positive and Gram-negative bacteria.
T3 353-951 Sentence denotes The biosynthetic pathway of the precursor molecule, GDP-D-perosamine, in Vibrio cholerae O1 starts with an isomerisation of fructose-6-phosphate catalyzed by the bifunctional enzyme phosphomannose isomerase-guanosine diphosphomannose pyrophosphorylase (RfbA; E.C. 2.7.7.22) creating the intermediate mannose-6-phosphate, which is subsequently converted by the phosphomanno-mutase (RfbB; E.C. 5.4.2.8) and further by RfbA to GDP-D-mannose, to GDP-4 keto-6-deoxymannose by a 4,6-dehydratase (RfbD; E.C. 4.2.1.47) and finally to GDP-D-perosamine by an aminotransferase (RfbE; E.C. not yet classified).
T4 952-1047 Sentence denotes We cloned the rfbD and the rfbE genes of V. cholerae O1 in Escherichia coli expression vectors.
T5 1048-1149 Sentence denotes Both biosynthetic enzymes were overproduced in E. coli BL21 (DE3) and their activities were analyzed.
T6 1150-1366 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-D-perosamine was optimized and the final product, GDP-D-perosamine, was purified and identified by nuclear magnetic resonance, mass spectrometry, and chromatography.
T7 1367-1486 Sentence denotes The catalytically active form of the GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase seems to be a tetramer of 170 kDa.
T8 1487-1625 Sentence denotes The His-tag RfbE fusion protein has a Km of 0.06 mM and a Vmax value of 38 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose.
T9 1626-1730 Sentence denotes The Km and Vmax values for the cosubstrate L-glutamate were 0.1 mM and 42 nkat/mg protein, respectively.
T10 1731-1927 Sentence denotes The intention of this work is to establish a basis for both the in vitro production of GDP-D-perosamine and for an in vivo perosaminylation system in a suitable bacterial host, preferably E. coli.
T1 0-134 Sentence denotes Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine.
T2 135-352 Sentence denotes The 4-amino-6-deoxy-monosaccharide D-perosamine is an important element in the glycosylation of interesting cell products, such as antibiotics and lipopolysaccharides (LPS) of Gram-positive and Gram-negative bacteria.
T3 353-616 Sentence denotes The biosynthetic pathway of the precursor molecule, GDP-D-perosamine, in Vibrio cholerae O1 starts with an isomerisation of fructose-6-phosphate catalyzed by the bifunctional enzyme phosphomannose isomerase-guanosine diphosphomannose pyrophosphorylase (RfbA; E.C.
T4 617-744 Sentence denotes 2.7.7.22) creating the intermediate mannose-6-phosphate, which is subsequently converted by the phosphomanno-mutase (RfbB; E.C.
T5 745-853 Sentence denotes 5.4.2.8) and further by RfbA to GDP-D-mannose, to GDP-4 keto-6-deoxymannose by a 4,6-dehydratase (RfbD; E.C.
T6 854-951 Sentence denotes 4.2.1.47) and finally to GDP-D-perosamine by an aminotransferase (RfbE; E.C. not yet classified).
T7 952-1047 Sentence denotes We cloned the rfbD and the rfbE genes of V. cholerae O1 in Escherichia coli expression vectors.
T8 1048-1149 Sentence denotes Both biosynthetic enzymes were overproduced in E. coli BL21 (DE3) and their activities were analyzed.
T9 1150-1366 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-D-perosamine was optimized and the final product, GDP-D-perosamine, was purified and identified by nuclear magnetic resonance, mass spectrometry, and chromatography.
T10 1367-1486 Sentence denotes The catalytically active form of the GDP-4-keto-6-deoxy-D-mannose-4-aminotransferase seems to be a tetramer of 170 kDa.
T11 1487-1625 Sentence denotes The His-tag RfbE fusion protein has a Km of 0.06 mM and a Vmax value of 38 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose.
T12 1626-1730 Sentence denotes The Km and Vmax values for the cosubstrate L-glutamate were 0.1 mM and 42 nkat/mg protein, respectively.
T13 1731-1927 Sentence denotes The intention of this work is to establish a basis for both the in vitro production of GDP-D-perosamine and for an in vivo perosaminylation system in a suitable bacterial host, preferably E. coli.

GO-BP

Id Subject Object Predicate Lexical cue
T1 104-113 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T2 104-120 http://purl.obolibrary.org/obo/GO_0046711 denotes synthesis of GDP
T3 104-122 http://purl.obolibrary.org/obo/GO_0019306 denotes synthesis of GDP-D
T4 214-227 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T5 1371-1391 http://purl.obolibrary.org/obo/GO_0003824 denotes catalytically active

GO-CC

Id Subject Object Predicate Lexical cue
T1 243-247 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2 1902-1906 http://purl.obolibrary.org/obo/GO_0018995 denotes host

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 42-49 http://edamontology.org/topic_0078 denotes protein
T2 370-377 http://edamontology.org/topic_0602 denotes pathway
T3 395-403 http://edamontology.org/topic_2839 denotes molecule
T4 1300-1326 http://edamontology.org/topic_3444 denotes nuclear magnetic resonance
T5 1328-1345 http://edamontology.org/topic_0134 denotes mass spectrometry
T6 1328-1345 http://edamontology.org/topic_3520 denotes mass spectrometry
T7 1511-1518 http://edamontology.org/topic_0078 denotes protein
T8 1570-1577 http://edamontology.org/topic_0078 denotes protein
T9 1708-1715 http://edamontology.org/topic_0078 denotes protein

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 42-49 http://edamontology.org/format_1208 denotes protein
T2 42-49 http://edamontology.org/data_1467 denotes protein
T3 155-169 http://edamontology.org/data_2746 denotes monosaccharide
T4 370-377 http://edamontology.org/data_2600 denotes pathway
T5 1164-1174 http://edamontology.org/operation_3434 denotes conversion
T6 1282-1296 http://edamontology.org/data_0992 denotes and identified
T7 1286-1296 http://edamontology.org/data_0842 denotes identified
T8 1286-1296 http://edamontology.org/data_2611 denotes identified
T9 1328-1345 http://edamontology.org/data_2536 denotes mass spectrometry
T10 1328-1345 http://edamontology.org/data_3147 denotes mass spectrometry
T11 1511-1518 http://edamontology.org/format_1208 denotes protein
T12 1511-1518 http://edamontology.org/data_1467 denotes protein
T13 1525-1527 http://edamontology.org/data_0910 denotes Km
T14 1545-1549 http://edamontology.org/data_0909 denotes Vmax
T15 1570-1577 http://edamontology.org/data_1467 denotes protein
T16 1570-1577 http://edamontology.org/format_1208 denotes protein
T17 1630-1632 http://edamontology.org/data_0910 denotes Km
T18 1637-1641 http://edamontology.org/data_0909 denotes Vmax
T19 1708-1715 http://edamontology.org/data_1467 denotes protein
T20 1708-1715 http://edamontology.org/format_1208 denotes protein

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 303-306 http://rdf.glycoinfo.org/glycan/G00022MO denotes LPS

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 55-70 Disease denotes Vibrio cholerae http://purl.obolibrary.org/obo/MONDO_0015766
T2 426-441 Disease denotes Vibrio cholerae http://purl.obolibrary.org/obo/MONDO_0015766

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 55-70 OrganismTaxon denotes Vibrio cholerae 666
T2 343-351 OrganismTaxon denotes bacteria 2|629395
T4 426-441 OrganismTaxon denotes Vibrio cholerae 666
T5 1011-1027 OrganismTaxon denotes Escherichia coli 562
T6 1095-1102 OrganismTaxon denotes E. coli 562
T7 1919-1926 OrganismTaxon denotes E. coli 562