PubMed:11459824 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11459824","sourcedb":"PubMed","sourceid":"11459824","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11459824","text":"Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene.\nPlants maintain cytosine methylation at CG and non-CG residues to control gene expression and genome stability. In a screen for Arabidopsis mutants that alter methylation and silencing of a densely methylated endogenous reporter gene, we recovered 11 loss-of-function alleles in the CMT3 chromomethylase gene. The cmt3 mutants displayed enhanced expression and reduced methylation of the reporter, particularly at non-CG cytosines. CNG methylation was also reduced at repetitive centromeric sequences. Thus, CMT3 is a key determinant for non-CG methylation. The lack of CMT homologs in animal genomes could account for the observation that in contrast to plants, animals maintain primarily CG methylation.","tracks":[{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":388,"end":392},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":613,"end":617},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":675,"end":678},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0007790"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0007790"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0015626"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PMID_GLOBAL","color":"#ecb293","default":true}]}]}}