PubMed:11410804 JSONTXT

{"project":"PennBioIE","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"protein"},{"id":"T2","span":{"begin":112,"end":117},"obj":"protein"},{"id":"T3","span":{"begin":293,"end":303},"obj":"protein"},{"id":"T4","span":{"begin":410,"end":415},"obj":"protein"},{"id":"T5","span":{"begin":416,"end":425},"obj":"protein"},{"id":"T6","span":{"begin":429,"end":435},"obj":"protein"},{"id":"T7","span":{"begin":440,"end":442},"obj":"protein"},{"id":"T8","span":{"begin":447,"end":449},"obj":"protein"},{"id":"T9","span":{"begin":451,"end":456},"obj":"protein"},{"id":"T10","span":{"begin":457,"end":466},"obj":"protein"},{"id":"T11","span":{"begin":682,"end":690},"obj":"protein"},{"id":"T12","span":{"begin":697,"end":698},"obj":"protein"},{"id":"T13","span":{"begin":701,"end":702},"obj":"protein"},{"id":"T14","span":{"begin":703,"end":715},"obj":"protein"},{"id":"T15","span":{"begin":752,"end":764},"obj":"protein"},{"id":"T16","span":{"begin":770,"end":777},"obj":"protein"},{"id":"T17","span":{"begin":784,"end":792},"obj":"protein"},{"id":"T18","span":{"begin":798,"end":804},"obj":"protein"},{"id":"T19","span":{"begin":855,"end":860},"obj":"protein"},{"id":"T20","span":{"begin":889,"end":890},"obj":"protein"},{"id":"T21","span":{"begin":893,"end":894},"obj":"protein"},{"id":"T22","span":{"begin":895,"end":907},"obj":"protein"},{"id":"T23","span":{"begin":923,"end":928},"obj":"protein"},{"id":"T24","span":{"begin":929,"end":938},"obj":"protein"},{"id":"T25","span":{"begin":1001,"end":1002},"obj":"protein"},{"id":"T26","span":{"begin":1005,"end":1006},"obj":"protein"},{"id":"T27","span":{"begin":1007,"end":1020},"obj":"protein"},{"id":"T28","span":{"begin":1069,"end":1074},"obj":"protein"}],"text":"K-ras gene mutations in non-small cell lung cancer in Japanese.\nTo evaluate the association of mutations in the K-ras gene with the incidence of non-small cell lung cancer (NSCLC) in Japanese patients, 410 patients treated surgically were studied. DNA was extracted from frozen specimens, and polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) hybridization assay was used to examine K-ras mutations in codons 12, 13 and 61. K-ras mutations were found in 33 patients (8.0%), and all were smokers or ex-smokers. There were no significant differences in clinical or pathological stage between wild-type cases and mutant cases. The most frequently identified mutation was a G--\u003eT transversion (25/33, 75.8%) that resulted in the substitution of a glycine for a cysteine or a valine. Previous studies have shown that the majority of K-ras mutations among smokers are G--\u003eT transversion. In our study, K-ras mutations were found only in smokers, and there was a high frequency of G--\u003eT transversions. A clear correlation exists between smoking and K-ras gene mutations."}