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PubMed:11371126 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 181-225 gene:8743 denotes TNF-related apoptosis-inducing ligand (TRAIL
T1 295-309 disease:C0029925 denotes ovarian cancer
T2 181-225 gene:8743 denotes TNF-related apoptosis-inducing ligand (TRAIL
T3 295-309 disease:C1140680 denotes ovarian cancer
R1 T0 T1 associated_with TNF-related apoptosis-inducing ligand (TRAIL,ovarian cancer
R2 T2 T3 associated_with TNF-related apoptosis-inducing ligand (TRAIL,ovarian cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
11371126-16#19#24#gene8743 1910-1915 gene8743 denotes TRAIL
11371126-16#90#105#diseaseC0029925 1981-1996 diseaseC0029925 denotes ovarian cancers
11371126-16#90#105#diseaseC1140680 1981-1996 diseaseC1140680 denotes ovarian cancers
19#24#gene874390#105#diseaseC0029925 11371126-16#19#24#gene8743 11371126-16#90#105#diseaseC0029925 associated_with TRAIL,ovarian cancers
19#24#gene874390#105#diseaseC1140680 11371126-16#19#24#gene8743 11371126-16#90#105#diseaseC1140680 associated_with TRAIL,ovarian cancers

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 132-342 OBJECTIVE denotes The aim of this study was to investigate whether TNF-related apoptosis-inducing ligand (TRAIL) alone or in combination with chemotherapy could induce apoptosis in ovarian cancer cells resistant to chemotherapy.
T2 352-1000 METHODS denotes Twelve chemoresistant epithelial cancer cell lines were treated with each chemotherapeutic drug alone (cisplatin, doxorubicin, or paclitaxel), TRAIL alone, or the combination. Toxicity was assessed using the MTS assay. To assess whether growth inhibition was due to apoptosis, TUNEL assay, caspase activation (measured by caspase-3 and PARP cleavage), and the sub G0/G1 fraction of cells were measured. Synergism was confirmed by fractional inhibition and dose-effect analysis. Expression of death and decoy receptors was studied by immunoblotting and an RNase protection assay. Statistical comparison of means was performed using Student's t test.
T3 1010-1617 RESULTS denotes The majority of the chemoresistant cells were also resistant to TRAIL alone. In contrast, the combination of TRAIL and chemotherapy resulted in a significant growth inhibition over a wide range of concentrations. This interaction was synergistic by dose-effect analysis. Flow cytometry demonstrated a significant increase in the fraction of apoptotic cells by the combination compared to each reagent alone. A significant enhancement in caspase and PARP cleavage was observed upon treatment with the combination. Finally, no correlation between induction of apoptosis and level of death receptors was found.
T4 1631-1997 CONCLUSIONS denotes The data suggest that almost all the ovarian cancer cells, which are resistant to chemotherapy, are also resistant to TRAIL. The combination of TRAIL and chemotherapy overcomes this resistance in a synergistic fashion by triggering caspase-mediated apoptosis. The combination of TRAIL and chemotherapy could be useful as a therapy for chemoresistant ovarian cancers.