PubMed:11360673 JSONTXT

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{"target":"","sourcedb":"PubMed","sourceid":"11360673","source_url":"","text":"Probucol inhibits lipid peroxidation of macrophage and affects its secretory properties.\nAIM: To investigate the mechanisms of anti-atherogenic actions of probucol.\nMETHODS: Human peripheral blood monocytes were cultured, and treated by copper ion (10 mumol/L) and/or probucol (PBC). Lipid peroxidation was measured by assaying malondialdehyde (MDA). The cytokine interleukin-1 beta (IL-1 beta) and apolipoprotein E (apo E) secreted by monocyte were assayed by enzyme linked immunoassay (ELISA).\nRESULTS: PBC 10-80 mumol/L inhibited copper ion-induced cellular lipid peroxidation from 15.30 to 7.74 mumol MDA/g cell protein. PBC 40 mumol/L inhibited oxidized macrophage-mediated oxidation of LDL from 5.18 to 1.65 mumol MDA/g cell protein, and attenuated secretory properties of monocytes induced by copper ion. The release of apo E, which is involved in reverse cholesterol transport, increased by 65%. And the release of IL-1 beta, which was shown to enhance vascular smooth muscle cell proliferation, decreased by 45%.\nCONCLUSION: Probucol inhibits lipid peroxidation of macrophages and affects their secretory properties.","tracks":[]}