| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-84 |
Sentence |
denotes |
Structural characterization of protein kinase A as a function of nucleotide binding. |
| T2 |
85-214 |
Sentence |
denotes |
Hydrogen-deuterium exchange studies using matrix-assisted laser desorption ionization-time of flight mass spectrometry detection. |
| T3 |
215-443 |
Sentence |
denotes |
Transient state kinetic studies indicate that substrate phosphorylation in protein kinase A is partially rate-limited by conformational changes, some of which may be associated with nucleotide binding (Shaffer, J., and Adams, J. |
| T4 |
444-484 |
Sentence |
denotes |
A. (1999) Biochemistry 38, 12072-12079). |
| T5 |
485-683 |
Sentence |
denotes |
To assess whether specific structural changes are associated with the binding of nucleotides, hydrogen-deuterium exchange experiments were performed on the enzyme in the absence and presence of ADP. |
| T6 |
684-763 |
Sentence |
denotes |
Four regions of the protein are protected from exchange in the presence of ADP. |
| T7 |
764-865 |
Sentence |
denotes |
Two regions encompass the catalytic and glycine-rich loops and are integral parts of the active site. |
| T8 |
866-970 |
Sentence |
denotes |
Conversely, protection of probes in the C terminus is consistent with nucleotide-induced domain closure. |
| T9 |
971-1209 |
Sentence |
denotes |
One protected probe encompasses a portion of helix C, a secondary structural element that does not make any direct contacts with the nucleotide but has been reported to undergo segmental motion upon the activation of some protein kinases. |
| T10 |
1210-1427 |
Sentence |
denotes |
The combined data suggest that binding of the nucleotide has distal structural effects that may include stabilizing the closed state of the enzyme and altering the position of a critical helix outside the active site. |
| T11 |
1428-1537 |
Sentence |
denotes |
The latter represents the first evidence that the nucleotide alone can induce changes in helix C in solution. |
