PubMed:11259485 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11259485","sourcedb":"PubMed","sourceid":"11259485","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11259485","text":"Preferential expression of antioxidant response element mediated gene expression in astrocytes.\nTranscriptional control of target genes by antioxidant/electrophile response elements has been well described in peripheral tissues. Genes that are regulated by this mechanism include the antioxidant enzymes NAD(P)H:quinone oxidoreductase, gamma-glutamyl cystine synthetase and glutathione-S-transferase. Antioxidant/electrophile response elements within a gene's promoter confer induction by low-molecular-weight electrophilic compounds such as tert-butylhydroquinone and dimethyl fumarate. We have now examined the ability of antioxidant/electrophile response elements to elicit gene expression in neurons and astrocytes in both brain slices and primary cultures using transient transfection of promoter reporter constructs. Our results using a heat-stable human placental alkaline phosphatase reporter indicate that antioxidant/electrophile response element mediated gene expression is largely restricted to astrocyte cell populations. Placental alkaline phosphatase expression was significantly elevated in astrocytes treated with the antioxidant/electrophile response element inducer dimethyl fumarate. Mutant constructs lacking a functional antioxidant/electrophile response element abolished all placental alkaline phosphatase expression in astrocytes. We suggest that astrocytic metabolic processes that normally aid and/or protect neurons may be controlled via this inducible system.","tracks":[{"project":"PennBioIE","denotations":[{"id":"T1","span":{"begin":304,"end":311},"obj":"protein"},{"id":"T2","span":{"begin":312,"end":334},"obj":"protein"},{"id":"T3","span":{"begin":336,"end":369},"obj":"protein"},{"id":"T4","span":{"begin":374,"end":399},"obj":"protein"},{"id":"T5","span":{"begin":861,"end":891},"obj":"protein"},{"id":"T6","span":{"begin":1035,"end":1065},"obj":"protein"},{"id":"T7","span":{"begin":1299,"end":1329},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"PennBioIE"},{"subj":"T2","pred":"source","obj":"PennBioIE"},{"subj":"T3","pred":"source","obj":"PennBioIE"},{"subj":"T4","pred":"source","obj":"PennBioIE"},{"subj":"T5","pred":"source","obj":"PennBioIE"},{"subj":"T6","pred":"source","obj":"PennBioIE"},{"subj":"T7","pred":"source","obj":"PennBioIE"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PennBioIE","color":"#bcec93","default":true}]}]}}