PubMed:11247601 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/11247601","sourcedb":"PubMed","sourceid":"11247601","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/11247601","text":"Protein phosphatase 2A: identification in Oryza sativa of the gene encoding the regulatory A subunit.\nA 2225 bp cDNA, designated RPA1, was isolated from an Oryza sativa cDNA library. Analysis revealed a 1761 bp coding sequence with 15 non-identical repeat units. The ORF encoded the A regulatory subunit of protein phosphatase 2A (PP2A-A) as ascertained by complementation of the yeast tpd3 mutant defective in this gene. The corresponding genomic DNA from a rice genome BAC library revealed that the gene contains eleven introns. The rice genome contains only a single copy of this gene as judged by Southern blot analysis. The PP2A protein is highly conserved in nature; the rice protein shows 88% amino acid identity with its counterparts in Arabidopsis or Nicotiana 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