PubMed:11235952
Annnotations
DisGeNET
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T0 | 54-94 | gene:7077 | denotes | tissue inhibitor of metalloproteinases 2 |
| T1 | 134-150 | disease:C0555198 | denotes | malignant glioma |
| T2 | 9-49 | gene:4323 | denotes | membrane type 1 matrix metalloproteinase |
| T3 | 134-150 | disease:C0555198 | denotes | malignant glioma |
| T4 | 1038-1045 | gene:4323 | denotes | MT1-MMP |
| T5 | 1076-1088 | disease:C0017636 | denotes | glioblastoma |
| T6 | 1491-1497 | gene:7077 | denotes | TIMP-2 |
| T7 | 1542-1554 | disease:C0017636 | denotes | glioblastoma |
| R1 | T0 | T1 | associated_with | tissue inhibitor of metalloproteinases 2,malignant glioma |
| R2 | T2 | T3 | associated_with | membrane type 1 matrix metalloproteinase,malignant glioma |
| R3 | T4 | T5 | associated_with | MT1-MMP,glioblastoma |
| R4 | T6 | T7 | associated_with | TIMP-2,glioblastoma |
DisGeNET5_gene_disease
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 11235952-0#9#49#gene4323 | 9-49 | gene4323 | denotes | membrane type 1 matrix metalloproteinase |
| 11235952-0#54#94#gene7077 | 54-94 | gene7077 | denotes | tissue inhibitor of metalloproteinases 2 |
| 11235952-0#134#150#diseaseC0555198 | 134-150 | diseaseC0555198 | denotes | malignant glioma |
| 11235952-12#28#35#gene4323 | 2170-2177 | gene4323 | denotes | MT1-MMP |
| 11235952-12#145#151#gene7077 | 2287-2293 | gene7077 | denotes | TIMP-2 |
| 11235952-12#94#106#diseaseC0017636 | 2236-2248 | diseaseC0017636 | denotes | glioblastoma |
| 11235952-4#111#117#gene7076 | 842-848 | gene7076 | denotes | TIMP-1 |
| 11235952-4#163#176#diseaseC0017636 | 894-907 | diseaseC0017636 | denotes | glioblastomas |
| 9#49#gene4323134#150#diseaseC0555198 | 11235952-0#9#49#gene4323 | 11235952-0#134#150#diseaseC0555198 | associated_with | membrane type 1 matrix metalloproteinase,malignant glioma |
| 54#94#gene7077134#150#diseaseC0555198 | 11235952-0#54#94#gene7077 | 11235952-0#134#150#diseaseC0555198 | associated_with | tissue inhibitor of metalloproteinases 2,malignant glioma |
| 28#35#gene432394#106#diseaseC0017636 | 11235952-12#28#35#gene4323 | 11235952-12#94#106#diseaseC0017636 | associated_with | MT1-MMP,glioblastoma |
| 145#151#gene707794#106#diseaseC0017636 | 11235952-12#145#151#gene7077 | 11235952-12#94#106#diseaseC0017636 | associated_with | TIMP-2,glioblastoma |
| 111#117#gene7076163#176#diseaseC0017636 | 11235952-4#111#117#gene7076 | 11235952-4#163#176#diseaseC0017636 | associated_with | TIMP-1,glioblastomas |
PubMed_Structured_Abstracts
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 160-721 | OBJECTIVE | denotes | Acquisition of invasive and metastatic potentials through proteinase expression is an essential event in tumor progression. Among proteinases, matrix metalloproteinases (MMPs) are thought to play a key role in tumor progression through the degradation of the extracellular matrix. In the present study, the authors examined the role of MMP-2 (gelatinase A) and membrane type 1 MMP (MT1-MMP), an activator of the zymogen of MMP-2, proMMP-2, together with tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in the invasion of astrocytic tumors in humans. |
| T2 | 731-2128 | METHODS | denotes | Analyses performed using sandwich enzyme immunoassays demonstrated that the production levels of pro-MMP-2 and TIMP-1, but not TIMP-2, are significantly higher in glioblastomas multiforme than in other grades of astrocytic tumors. Quantitative reverse transcription-polymerase chain reaction indicated that MT1-MMP is expressed predominantly in glioblastoma tissues, and its expression levels are significantly enhanced as tumor grade increases. In addition, the expression levels and proMMP-2 activation ratio were remarkably higher in glioblastomas associated with cerebrospinal fluid (CSF) dissemination than in those not associated with CSF dissemination. In contrast, an examination of TIMP-2 levels showed a reverse correlation. Like MT1-MMP, TIMP-1 and TIMP-2 were immunolocalized to neoplastic cells in glioblastoma samples. To study the roles of these molecules in the invasion of astrocytic tumors more fully, stable transfectants expressing the MT1-MMP gene were developed in a U251 human glioblastoma cell line. The MT1-MMP transfectants displayed prominent activation of proMMP-2 and invasive growth in three-dimensional collagen gel; however, mock transfectants and parental cells displayed noninvasive growth without the activation. The invasion and gelatinolytic activity of the transfectants were completely inhibited by addition of recombinant TIMP-2, but not recombinant TIMP-1. |
| T3 | 2142-2294 | CONCLUSIONS | denotes | These results indicate that MT1-MMP may contribute to tumor invasion and CSF dissemination of glioblastoma cells on the basis of an imbalance of TIMP-2. |