| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-108 |
Sentence |
denotes |
Resistance to TRAIL-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression. |
| TextSentencer_T2 |
109-120 |
Sentence |
denotes |
BACKGROUND: |
| TextSentencer_T3 |
121-249 |
Sentence |
denotes |
Disruption of apoptotic pathways may be involved in tumor formation, regression, and treatment resistance of neuroblastoma (NB). |
| TextSentencer_T4 |
250-350 |
Sentence |
denotes |
TNF-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in cancer cell lines. |
| TextSentencer_T5 |
351-361 |
Sentence |
denotes |
PROCEDURE: |
| TextSentencer_T6 |
362-535 |
Sentence |
denotes |
In this study we analyzed the expression and function of TRAIL, its agonistic and antagonistic receptors, and important intracellular signaling elements in 18 NB cell lines. |
| TextSentencer_T7 |
536-544 |
Sentence |
denotes |
RESULTS: |
| TextSentencer_T8 |
545-651 |
Sentence |
denotes |
Semiquantitative RT-PCR revealed that TRAIL-R2 and TRAIL-R3 are the main TRAIL-receptors used by NB cells. |
| TextSentencer_T9 |
652-758 |
Sentence |
denotes |
Sensitivity to TRAIL-induced apoptosis did not correlate with mRNA expression of TRAIL receptors or cFLIP. |
| TextSentencer_T10 |
759-846 |
Sentence |
denotes |
Surprisingly, caspase-8 and caspase-10 mRNA was detected in only 5 of 18 NB cell lines. |
| TextSentencer_T11 |
847-973 |
Sentence |
denotes |
Interestingly, only these five NB cell lines were susceptible to TRAIL-induced apoptosis in a time- and dose-dependent manner. |
| TextSentencer_T12 |
974-986 |
Sentence |
denotes |
CONCLUSIONS: |
| TextSentencer_T13 |
987-1187 |
Sentence |
denotes |
Treatment with 5-aza-2'-deoxycytidine restored mRNA expression of caspase-8 and -10 and TRAIL sensitivity of resistant cell lines, suggesting that gene methylation is involved in caspase inactivation. |
| TextSentencer_T14 |
1188-1366 |
Sentence |
denotes |
Since many cytotoxic drugs induce caspase-dependent apoptosis, failure to express caspase-8 and/or caspase-10 might be an important mechanism of resistance to chemotherapy in NB. |
| T1 |
0-108 |
Sentence |
denotes |
Resistance to TRAIL-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression. |
| T2 |
109-120 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
121-249 |
Sentence |
denotes |
Disruption of apoptotic pathways may be involved in tumor formation, regression, and treatment resistance of neuroblastoma (NB). |
| T4 |
250-350 |
Sentence |
denotes |
TNF-related apoptosis-inducing ligand (TRAIL) is a potent inducer of apoptosis in cancer cell lines. |
| T5 |
351-361 |
Sentence |
denotes |
PROCEDURE: |
| T6 |
362-535 |
Sentence |
denotes |
In this study we analyzed the expression and function of TRAIL, its agonistic and antagonistic receptors, and important intracellular signaling elements in 18 NB cell lines. |
| T7 |
536-544 |
Sentence |
denotes |
RESULTS: |
| T8 |
545-651 |
Sentence |
denotes |
Semiquantitative RT-PCR revealed that TRAIL-R2 and TRAIL-R3 are the main TRAIL-receptors used by NB cells. |
| T9 |
652-758 |
Sentence |
denotes |
Sensitivity to TRAIL-induced apoptosis did not correlate with mRNA expression of TRAIL receptors or cFLIP. |
| T10 |
759-846 |
Sentence |
denotes |
Surprisingly, caspase-8 and caspase-10 mRNA was detected in only 5 of 18 NB cell lines. |
| T11 |
847-973 |
Sentence |
denotes |
Interestingly, only these five NB cell lines were susceptible to TRAIL-induced apoptosis in a time- and dose-dependent manner. |
| T12 |
974-986 |
Sentence |
denotes |
CONCLUSIONS: |
| T13 |
987-1187 |
Sentence |
denotes |
Treatment with 5-aza-2'-deoxycytidine restored mRNA expression of caspase-8 and -10 and TRAIL sensitivity of resistant cell lines, suggesting that gene methylation is involved in caspase inactivation. |
| T14 |
1188-1366 |
Sentence |
denotes |
Since many cytotoxic drugs induce caspase-dependent apoptosis, failure to express caspase-8 and/or caspase-10 might be an important mechanism of resistance to chemotherapy in NB. |