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GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 88-93 FMAID:169002 denotes cells
_T2 88-93 FMAID:68646 denotes cells
_T3 485-493 FMAID:165447 denotes proteins
_T4 485-493 FMAID:67257 denotes proteins
_T5 533-538 FMAID:68646 denotes cells
_T6 533-538 FMAID:169002 denotes cells
_T7 860-869 FMAID:162299 denotes lysosomes
_T8 860-869 FMAID:63836 denotes lysosomes
_T9 912-920 FMAID:67257 denotes proteins
_T10 912-920 FMAID:165447 denotes proteins
_T11 1434-1443 FMAID:162299 denotes lysosomal
_T12 1434-1443 FMAID:63836 denotes lysosomal

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 88-93 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T2 533-538 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T3 978-986 http://purl.bioontology.org/ontology/STY/T033 denotes findings
T4 1345-1353 http://purl.bioontology.org/ontology/STY/T033 denotes findings

GO-BP

Id Subject Object Predicate Lexical cue
T1 691-704 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation

GO-CC

Id Subject Object Predicate Lexical cue
T1 88-93 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2 533-538 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3 860-869 http://purl.obolibrary.org/obo/GO_0005764 denotes lysosomes
T4 1434-1443 http://purl.obolibrary.org/obo/GO_0005764 denotes lysosomal

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 0-5 http://edamontology.org/topic_2815 denotes Human
T2 101-115 http://edamontology.org/topic_0091 denotes bioinformatics
T3 261-266 http://edamontology.org/topic_2815 denotes human
T4 332-339 http://edamontology.org/topic_3678 denotes studies
T5 485-493 http://edamontology.org/topic_0078 denotes proteins
T6 664-671 http://edamontology.org/topic_3678 denotes studies
T7 755-765 http://edamontology.org/topic_3382 denotes microscopy
T8 860-869 http://edamontology.org/topic_0616 denotes lysosomes
T9 912-920 http://edamontology.org/topic_0078 denotes proteins
T10 1055-1060 http://edamontology.org/topic_2815 denotes human
T11 1311-1330 http://edamontology.org/topic_3361 denotes labeling techniques
T12 1415-1422 http://edamontology.org/topic_0634 denotes disease
T13 1428-1433 http://edamontology.org/topic_2815 denotes human
T14 1434-1443 http://edamontology.org/topic_0616 denotes lysosomal
T15 1444-1451 http://edamontology.org/topic_0634 denotes disease

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 144-154 http://edamontology.org/data_0842 denotes identified
T2 144-154 http://edamontology.org/data_2611 denotes identified
T3 389-400 http://edamontology.org/operation_2423 denotes predictions
T4 485-493 http://edamontology.org/data_1467 denotes proteins
T5 485-493 http://edamontology.org/format_1208 denotes proteins
T6 634-640 http://edamontology.org/data_2048 denotes report
T7 705-713 http://edamontology.org/operation_2945 denotes analysis
T8 832-841 http://edamontology.org/operation_0004 denotes processed
T9 832-841 http://edamontology.org/operation_2409 denotes processed
T10 912-920 http://edamontology.org/data_1467 denotes proteins
T11 912-920 http://edamontology.org/format_1208 denotes proteins
T12 960-970 http://edamontology.org/operation_0004 denotes processing
T13 960-970 http://edamontology.org/operation_2409 denotes processing
T14 1186-1197 http://edamontology.org/operation_2423 denotes predictions

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
TextSentencer_T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
TextSentencer_T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
TextSentencer_T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
TextSentencer_T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
TextSentencer_T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
TextSentencer_T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
TextSentencer_T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
TextSentencer_T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
TextSentencer_T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.
T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.
T1 0-94 Sentence denotes Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells.
T2 95-286 Sentence denotes Using bioinformatics methods, we have previously identified Glu235 and Glu340 as the putative acid/base catalyst and nucleophile, respectively, in the active site of human glucocerebrosidase.
T3 287-401 Sentence denotes Thus, we undertook site-directed mutagenesis studies to obtain experimental evidence supporting these predictions.
T4 402-539 Sentence denotes Recombinant retroviruses were used to express wild-type and E235A and E340A mutant proteins in glucocerebrosidase-deficient murine cells.
T5 540-625 Sentence denotes In contrast to wild-type enzyme, the mutants were found to be catalytically inactive.
T6 626-870 Sentence denotes We also report the results of various studies (Western blotting, glycosylation analysis, subcellular fractionation, and confocal microscopy) indicating that the wild-type and mutant enzymes are identically processed and sorted to the lysosomes.
T7 871-971 Sentence denotes Thus, enzymatic inactivity of the mutant proteins is not the result of incorrect folding/processing.
T8 972-1121 Sentence denotes These findings indicate that Glu235 plays a key role in the catalytic machinery of human glucocerebrosidase and may indeed be the acid/base catalyst.
T9 1122-1331 Sentence denotes As concerns Glu340, the results both support our computer-based predictions and confirm, at the biological level, previous identification of Glu340 as the nucleophile by use of active site labeling techniques.
T10 1332-1500 Sentence denotes Finally, our findings may help to better understand the molecular basis of Gaucher disease, the human lysosomal disease resulting from deficiency in glucocerebrosidase.

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 1311-1319 id denotes labeling

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 497-525 Disease denotes glucocerebrosidase-deficient http://purl.obolibrary.org/obo/MONDO_0009265|http://purl.obolibrary.org/obo/MONDO_0018150
T3 1407-1422 Disease denotes Gaucher disease http://purl.obolibrary.org/obo/MONDO_0018150
T4 1434-1451 Disease denotes lysosomal disease http://purl.obolibrary.org/obo/MONDO_0002561

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 887-897 Phenotype denotes inactivity HP:0001254

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 0-5 OrganismTaxon denotes Human 9606
T2 261-266 OrganismTaxon denotes human 9606
T3 1055-1060 OrganismTaxon denotes human 9606
T4 1428-1433 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 860-869 Body_part denotes lysosomes http://purl.obolibrary.org/obo/GO_0005764

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 83-93 Cell denotes null cells http://purl.obolibrary.org/obo/CL:0000623|http://purl.obolibrary.org/obo/CL:0000825