PubMed:11030750
Annnotations
Glycan-Motif
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 69-75 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T2 | 96-103 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T3 | 260-266 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T4 | 375-381 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T5 | 550-557 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T6 | 605-612 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T7 | 686-693 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T8 | 701-707 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T9 | 785-792 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T10 | 862-868 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T11 | 941-948 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T12 | 960-966 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T13 | 996-1002 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T14 | 1054-1068 | https://glytoucan.org/Structures/Glycans/G00054MO | denotes | sialyl Lewis x |
| T15 | 1070-1074 | https://glytoucan.org/Structures/Glycans/G00054MO | denotes | sLex |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 69-75 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T2 | 96-103 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T3 | 260-266 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T4 | 375-381 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T5 | 550-557 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T6 | 605-612 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T7 | 686-693 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T8 | 701-707 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T9 | 785-792 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T10 | 862-868 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T11 | 941-948 | Glycan_Motif | denotes | mannose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ |
| T12 | 960-966 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T13 | 996-1002 | Glycan_Motif | denotes | fucose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO |
| T14 | 1054-1068 | Glycan_Motif | denotes | sialyl Lewis x | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO |
| T15 | 1061-1068 | Glycan_Motif | denotes | Lewis x | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G01187XC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00051MO |
| T17 | 1070-1074 | Glycan_Motif | denotes | sLex | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00054MO |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-132 | Sentence | denotes | Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae. |
| TextSentencer_T2 | 133-280 | Sentence | denotes | Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. |
| TextSentencer_T3 | 281-400 | Sentence | denotes | Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. |
| TextSentencer_T4 | 401-732 | Sentence | denotes | Here we describe the construction of a stable recombinant S.cerevisiae strain expressing the E.coli genes gmd and wcaG encoding the two enzymes, GDP-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase/4-reductase (GMER(FX)) respectively, needed to convert GDP-mannose to GDP-fucose via the de novo pathway. |
| TextSentencer_T5 | 733-949 | Sentence | denotes | Taking advantage of the rich inherent cytosolic GDP-mannose pool in S.cerevisiae cells we could easily produce 0.2 mg/l of GDP-L-fucose with this recombinant yeast strain without addition of any external GDP-mannose. |
| TextSentencer_T6 | 950-1139 | Sentence | denotes | The GDP-L-fucose product could be used as the fucose donor for alpha1,3fucosyltransferase to synthesize sialyl Lewis x (sLex), a glycan crucial for the selectin-dependent leukocyte traffic. |
| T1 | 0-132 | Sentence | denotes | Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae. |
| T2 | 133-280 | Sentence | denotes | Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. |
| T3 | 281-400 | Sentence | denotes | Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. |
| T4 | 401-732 | Sentence | denotes | Here we describe the construction of a stable recombinant S.cerevisiae strain expressing the E.coli genes gmd and wcaG encoding the two enzymes, GDP-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase/4-reductase (GMER(FX)) respectively, needed to convert GDP-mannose to GDP-fucose via the de novo pathway. |
| T5 | 733-949 | Sentence | denotes | Taking advantage of the rich inherent cytosolic GDP-mannose pool in S.cerevisiae cells we could easily produce 0.2 mg/l of GDP-L-fucose with this recombinant yeast strain without addition of any external GDP-mannose. |
| T6 | 950-1139 | Sentence | denotes | The GDP-L-fucose product could be used as the fucose donor for alpha1,3fucosyltransferase to synthesize sialyl Lewis x (sLex), a glycan crucial for the selectin-dependent leukocyte traffic. |
| T1 | 0-132 | Sentence | denotes | Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae. |
| T2 | 133-280 | Sentence | denotes | Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. |
| T3 | 281-400 | Sentence | denotes | Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. |
| T4 | 401-732 | Sentence | denotes | Here we describe the construction of a stable recombinant S.cerevisiae strain expressing the E.coli genes gmd and wcaG encoding the two enzymes, GDP-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase/4-reductase (GMER(FX)) respectively, needed to convert GDP-mannose to GDP-fucose via the de novo pathway. |
| T5 | 733-949 | Sentence | denotes | Taking advantage of the rich inherent cytosolic GDP-mannose pool in S.cerevisiae cells we could easily produce 0.2 mg/l of GDP-L-fucose with this recombinant yeast strain without addition of any external GDP-mannose. |
| T6 | 950-1139 | Sentence | denotes | The GDP-L-fucose product could be used as the fucose donor for alpha1,3fucosyltransferase to synthesize sialyl Lewis x (sLex), a glycan crucial for the selectin-dependent leukocyte traffic. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 69-75 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T2 | 96-103 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T3 | 260-266 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T4 | 375-381 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T5 | 550-557 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T6 | 605-612 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T7 | 686-693 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T8 | 701-707 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T9 | 785-792 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T10 | 862-868 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T11 | 941-948 | https://glytoucan.org/Structures/Glycans/G70323CJ | denotes | mannose |
| T12 | 960-966 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T13 | 996-1002 | https://glytoucan.org/Structures/Glycans/G82576YO | denotes | fucose |
| T14 | 1054-1068 | https://glytoucan.org/Structures/Glycans/G00054MO | denotes | sialyl Lewis x |
| T15 | 1061-1068 | https://glytoucan.org/Structures/Glycans/G00051MO | denotes | Lewis x |
| T16 | 1061-1068 | https://glytoucan.org/Structures/Glycans/G01187XC | denotes | Lewis x |
| T17 | 1070-1074 | https://glytoucan.org/Structures/Glycans/G00054MO | denotes | sLex |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1054-1068 | http://www.glycoepitope.jp/epitopes/EP0012 | denotes | sialyl Lewis x |
| T2 | 1061-1068 | http://www.glycoepitope.jp/epitopes/EP0011 | denotes | Lewis x |
| T3 | 1070-1074 | http://www.glycoepitope.jp/epitopes/EP0012 | denotes | sLex |
GlycoBiology-PACDB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 | denotes | Escherichia coli |
| _T2 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 | denotes | Escherichia coli |
| _T3 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 | denotes | Escherichia coli |
| _T4 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 | denotes | Escherichia coli |
| _T5 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 | denotes | Escherichia coli |
| _T6 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 | denotes | Escherichia coli |
| _T7 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 | denotes | Escherichia coli |
| _T8 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 | denotes | Escherichia coli |
| _T9 | 25-41 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 | denotes | Escherichia coli |
| _T10 | 107-131 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC297 | denotes | Saccharomyces cerevisiae |
GlycoBiology-FMA
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 69-75 | FMAID:196784 | denotes | fucose |
| _T2 | 69-75 | FMAID:82790 | denotes | fucose |
| _T3 | 96-103 | FMAID:196796 | denotes | mannose |
| _T4 | 96-103 | FMAID:82801 | denotes | mannose |
| _T5 | 160-173 | FMAID:167256 | denotes | glycoproteins |
| _T6 | 160-173 | FMAID:62925 | denotes | glycoproteins |
| _T7 | 179-185 | FMAID:67264 | denotes | lipids |
| _T8 | 179-185 | FMAID:165448 | denotes | lipids |
| _T9 | 260-266 | FMAID:196784 | denotes | fucose |
| _T10 | 260-266 | FMAID:82790 | denotes | fucose |
| _T11 | 375-381 | FMAID:196784 | denotes | fucose |
| _T12 | 375-381 | FMAID:82790 | denotes | fucose |
| _T13 | 501-506 | FMAID:198663 | denotes | genes |
| _T14 | 550-557 | FMAID:82801 | denotes | mannose |
| _T15 | 550-557 | FMAID:196796 | denotes | mannose |
| _T16 | 605-612 | FMAID:196796 | denotes | mannose |
| _T17 | 605-612 | FMAID:82801 | denotes | mannose |
| _T18 | 686-693 | FMAID:82801 | denotes | mannose |
| _T19 | 686-693 | FMAID:196796 | denotes | mannose |
| _T20 | 701-707 | FMAID:82790 | denotes | fucose |
| _T21 | 701-707 | FMAID:196784 | denotes | fucose |
| _T22 | 785-792 | FMAID:82801 | denotes | mannose |
| _T23 | 785-792 | FMAID:196796 | denotes | mannose |
| _T24 | 814-819 | FMAID:169002 | denotes | cells |
| _T25 | 814-819 | FMAID:68646 | denotes | cells |
| _T26 | 862-868 | FMAID:196784 | denotes | fucose |
| _T27 | 862-868 | FMAID:82790 | denotes | fucose |
| _T28 | 928-936 | FMAID:214709 | denotes | external |
| _T29 | 941-948 | FMAID:196796 | denotes | mannose |
| _T30 | 941-948 | FMAID:82801 | denotes | mannose |
| _T31 | 960-966 | FMAID:196784 | denotes | fucose |
| _T32 | 960-966 | FMAID:82790 | denotes | fucose |
| _T33 | 996-1002 | FMAID:82790 | denotes | fucose |
| _T34 | 996-1002 | FMAID:196784 | denotes | fucose |
| _T35 | 1102-1110 | FMAID:61795 | denotes | selectin |
| _T36 | 1102-1110 | FMAID:165243 | denotes | selectin |
| _T37 | 1121-1130 | FMAID:62852 | denotes | leukocyte |
| _T38 | 1121-1130 | FMAID:167148 | denotes | leukocyte |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 575-578 | http://www.uniprot.org/uniprot/O60547 | denotes | GMD |
| T2 | 645-647 | http://www.uniprot.org/uniprot/Q13630 | denotes | FX |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 507-510 | http://www.uniprot.org/uniprot/Q8K0C9 | denotes | gmd |
| T2 | 575-578 | http://www.uniprot.org/uniprot/Q8K0C9 | denotes | GMD |
| T3 | 645-647 | http://www.uniprot.org/uniprot/P23591 | denotes | FX |
GlycoBiology-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 25-36 | http://purl.bioontology.org/ontology/NCBITAXON/561 | denotes | Escherichia |
| T2 | 107-120 | http://purl.bioontology.org/ontology/NCBITAXON/4930 | denotes | Saccharomyces |
| T3 | 107-120 | http://purl.bioontology.org/ontology/NCBITAXON/4895 | denotes | Saccharomyces |
| T4 | 107-120 | http://purl.bioontology.org/ontology/NCBITAXON/36034 | denotes | Saccharomyces |
| T5 | 107-120 | http://purl.bioontology.org/ontology/NCBITAXON/4891 | denotes | Saccharomyces |
| T6 | 814-819 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 133-145 | http://purl.obolibrary.org/obo/GO_0036065 | denotes | Fucosylation |
| T2 | 317-328 | http://purl.obolibrary.org/obo/GO_0036065 | denotes | fucosylated |
| T3 | 507-510 | http://purl.obolibrary.org/obo/GO_0008446 | denotes | gmd |
| T4 | 575-578 | http://purl.obolibrary.org/obo/GO_0008446 | denotes | GMD |
| T5 | 1054-1060 | http://purl.obolibrary.org/obo/GO_0097503 | denotes | sialyl |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1102-1110 | http://purl.obolibrary.org/obo/GO_0030246 | denotes | selectin |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 771-780 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosolic |
| T2 | 814-819 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
EDAM-topics
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 179-185 | http://edamontology.org/topic_0153 | denotes | lipids |
| T2 | 724-731 | http://edamontology.org/topic_0602 | denotes | pathway |
| T3 | 891-896 | http://edamontology.org/topic_0782 | denotes | yeast |
| T4 | 891-896 | http://edamontology.org/topic_2817 | denotes | yeast |
EDAM-DFO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 422-434 | http://edamontology.org/operation_3429 | denotes | construction |
| T2 | 724-731 | http://edamontology.org/data_2600 | denotes | pathway |
GlycoBiology-Motifs
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1054-1066 | http://rdf.glycoinfo.org/glycan/G00053MO | denotes | sialyl Lewis |
| T2 | 1054-1068 | http://rdf.glycoinfo.org/glycan/G00054MO | denotes | sialyl Lewis x |
| T3 | 1061-1066 | http://rdf.glycoinfo.org/glycan/G00047MO | denotes | Lewis |
| T4 | 1061-1068 | http://rdf.glycoinfo.org/glycan/G00051MO | denotes | Lewis x |
| T5 | 1070-1074 | http://rdf.glycoinfo.org/glycan/G00054MO | denotes | sLex |
Lectin
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| Lectin_T1 | 1102-1110 | https://acgg.asia/db/lfdb/LfDB0142 | denotes | selectin |
| Lectin_T2 | 1102-1110 | https://acgg.asia/db/lfdb/LfDB0013 | denotes | selectin |
| Lectin_T3 | 1102-1110 | https://acgg.asia/db/lfdb/LfDB0043 | denotes | selectin |
GlycoBiology-Epitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-GlycoEpitope-B_T1 | 1061-1068 | http://www.glycoepitope.jp/epitopes/EP0011 | denotes | Lewis x |
| PD-GlycoEpitope-B_T2 | 1054-1066 | http://www.glycoepitope.jp/epitopes/EP0008 | denotes | sialyl Lewis |
| PD-GlycoEpitope-B_T3 | 1054-1068 | http://www.glycoepitope.jp/epitopes/EP0012 | denotes | sialyl Lewis x |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 1054-1068 | Glycan | denotes | sialyl Lewis x | https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO |
| T2 | 1070-1074 | Glycan | denotes | sLex | https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 1054-1068 | Glycan | denotes | sialyl Lewis x | https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO |
| T2 | 1070-1074 | Glycan | denotes | sLex | https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 1054-1068 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | sialyl Lewis x | http://www.glycoepitope.jp/epitopes/EP0012 |
| T2 | 1070-1074 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | sLex | http://www.glycoepitope.jp/epitopes/EP0012 |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 1121-1130 | Cell | denotes | leukocyte | http://purl.obolibrary.org/obo/CL:0000738 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 1121-1130 | Body_part | denotes | leukocyte | http://purl.obolibrary.org/obo/CL_0000738 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 25-41 | Organism | denotes | Escherichia coli | 562 |
| T2 | 107-131 | Organism | denotes | Saccharomyces cerevisiae | 4932 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-132 | Sentence | denotes | Functional expression of Escherichia coli enzymes synthesizing GDP-L-fucose from inherent GDP-D-mannose in Saccharomyces cerevisiae. |
| T2 | 133-280 | Sentence | denotes | Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. |
| T3 | 281-400 | Sentence | denotes | Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. |
| T4 | 401-732 | Sentence | denotes | Here we describe the construction of a stable recombinant S.cerevisiae strain expressing the E.coli genes gmd and wcaG encoding the two enzymes, GDP-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase/4-reductase (GMER(FX)) respectively, needed to convert GDP-mannose to GDP-fucose via the de novo pathway. |
| T5 | 733-949 | Sentence | denotes | Taking advantage of the rich inherent cytosolic GDP-mannose pool in S.cerevisiae cells we could easily produce 0.2 mg/l of GDP-L-fucose with this recombinant yeast strain without addition of any external GDP-mannose. |
| T6 | 950-1139 | Sentence | denotes | The GDP-L-fucose product could be used as the fucose donor for alpha1,3fucosyltransferase to synthesize sialyl Lewis x (sLex), a glycan crucial for the selectin-dependent leukocyte traffic. |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 1054-1068 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | sialyl Lewis x | http://www.glycoepitope.jp/epitopes/EP0012 |
| T2 | 1070-1074 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | sLex | http://www.glycoepitope.jp/epitopes/EP0012 |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 771-780 | Body_part | denotes | cytosolic | FMA:66836 |
| T2 | 1121-1130 | Body_part | denotes | leukocyte | FMA:62852 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 25-41 | OrganismTaxon | denotes | Escherichia coli | 562 |
| T2 | 107-131 | OrganismTaxon | denotes | Saccharomyces cerevisiae | 4932 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 1121-1130 | Body_part | denotes | leukocyte | http://purl.obolibrary.org/obo/CL_0000738 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 1121-1130 | Cell | denotes | leukocyte | http://purl.obolibrary.org/obo/CL:0000738 |