PubMed:11030748 / 0-760 JSONTXT

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{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":0,"end":8},"obj":"http://edamontology.org/data_2042"},{"id":"T2","span":{"begin":82,"end":92},"obj":"http://edamontology.org/operation_3434"},{"id":"T3","span":{"begin":223,"end":230},"obj":"http://edamontology.org/data_2600"},{"id":"T4","span":{"begin":269,"end":276},"obj":"http://edamontology.org/data_2048"},{"id":"T5","span":{"begin":340,"end":348},"obj":"http://edamontology.org/operation_2423"},{"id":"T6","span":{"begin":394,"end":400},"obj":"http://edamontology.org/data_2048"},{"id":"T7","span":{"begin":554,"end":564},"obj":"http://edamontology.org/operation_3434"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":375,"end":380},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":46,"end":62},"obj":"http://www.glycoepitope.jp/epitopes/EP0078"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":521,"end":537},"obj":"http://www.glycoepitope.jp/epitopes/EP0078"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":375,"end":380},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":375,"end":380},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":46,"end":62},"obj":"Glycan"},{"id":"T2","span":{"begin":78,"end":81},"obj":"Glycan"},{"id":"T3","span":{"begin":106,"end":109},"obj":"Glycan"},{"id":"T4","span":{"begin":299,"end":302},"obj":"Glycan"},{"id":"T5","span":{"begin":476,"end":479},"obj":"Glycan"},{"id":"T6","span":{"begin":521,"end":537},"obj":"Glycan"},{"id":"T7","span":{"begin":539,"end":545},"obj":"Glycan"},{"id":"T8","span":{"begin":550,"end":553},"obj":"Glycan"},{"id":"T9","span":{"begin":659,"end":662},"obj":"Glycan"},{"id":"T10","span":{"begin":699,"end":702},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A20","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A21","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A22","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A23","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A24","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A25","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A26","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A27","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A9","pred":"glycosmos_id","subj":"T9","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A28","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A10","pred":"glycosmos_id","subj":"T10","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A29","pred":"image","subj":"T10","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":371,"end":374},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":568,"end":583},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10029"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":27,"end":32},"obj":"Body_part"},{"id":"T2","span":{"begin":217,"end":222},"obj":"Body_part"},{"id":"T3","span":{"begin":362,"end":367},"obj":"Body_part"},{"id":"T4","span":{"begin":375,"end":380},"obj":"Body_part"},{"id":"T5","span":{"begin":426,"end":431},"obj":"Body_part"},{"id":"T6","span":{"begin":584,"end":589},"obj":"Body_part"},{"id":"T7","span":{"begin":624,"end":629},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0000992"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/GO_0005794"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":375,"end":380},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000097"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":94,"end":237},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":238,"end":432},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":433,"end":630},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":237},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":237},"obj":"Sentence"},{"id":"T3","span":{"begin":238,"end":432},"obj":"Sentence"},{"id":"T4","span":{"begin":433,"end":630},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":93},"obj":"Sentence"},{"id":"T2","span":{"begin":94,"end":237},"obj":"Sentence"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":46,"end":62},"obj":"Glycan"},{"id":"T2","span":{"begin":78,"end":81},"obj":"Glycan"},{"id":"T3","span":{"begin":106,"end":109},"obj":"Glycan"},{"id":"T4","span":{"begin":299,"end":302},"obj":"Glycan"},{"id":"T5","span":{"begin":476,"end":479},"obj":"Glycan"},{"id":"T6","span":{"begin":521,"end":537},"obj":"Glycan"},{"id":"T7","span":{"begin":539,"end":545},"obj":"Glycan"},{"id":"T8","span":{"begin":550,"end":553},"obj":"Glycan"},{"id":"T9","span":{"begin":659,"end":662},"obj":"Glycan"},{"id":"T10","span":{"begin":699,"end":702},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G84224TW"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A9","pred":"glycosmos_id","subj":"T9","obj":"https://glycosmos.org/glycans/show/G91237TK"},{"id":"A10","pred":"glycosmos_id","subj":"T10","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A20","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A21","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A22","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A23","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A24","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A25","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A26","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW"},{"id":"A27","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A28","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G91237TK"},{"id":"A29","pred":"image","subj":"T10","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":46,"end":62},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":521,"end":537},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":539,"end":545},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0078"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0078"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0078"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":371,"end":374},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":568,"end":583},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10029"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":46,"end":62},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":521,"end":537},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":539,"end":545},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0078"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0078"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0078"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":27,"end":32},"obj":"Body_part"},{"id":"T2","span":{"begin":217,"end":222},"obj":"Body_part"},{"id":"T3","span":{"begin":362,"end":367},"obj":"Body_part"},{"id":"T4","span":{"begin":375,"end":380},"obj":"Body_part"},{"id":"T5","span":{"begin":426,"end":431},"obj":"Body_part"},{"id":"T6","span":{"begin":584,"end":589},"obj":"Body_part"},{"id":"T7","span":{"begin":624,"end":629},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/GO_0005794"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0000992"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/GO_0005794"}],"text":"Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion.\nGanglioside GM2 synthase and other enzymes required for complex ganglioside synthesis were localized recently to the trans Golgi network (TGN). However, there are conflicting reports as to the location of GM3 synthase; originally this enzyme was detected in the early Golgi of rat liver but a recent report localized it to the late Golgi. We have used chimeric forms of ganglioside GM2 synthase to determine if the location of lactosylceramide (LacCer) to GM3 conversion in Chinese hamster ovary (CHO) cells was the early or late Golgi. Our approach tested whether GM3 could be utilized as a substrate by GM2 synthase chimeras which were targeted to compartments ear"}