PubMed:11005572 JSONTXT

{"project":"GlyCosmos15-Species","denotations":[{"id":"4","span":{"begin":54,"end":76},"obj":"Species"},{"id":"26","span":{"begin":123,"end":145},"obj":"Species"}],"attributes":[{"id":"A4","pred":"db_id","subj":"4","obj":"487"},{"id":"A26","pred":"db_id","subj":"26","obj":"487"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":710,"end":716},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":710,"end":716},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":122},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":123,"end":496},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":497,"end":660},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":661,"end":804},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":805,"end":936},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":937,"end":1036},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":122},"obj":"Sentence"},{"id":"T2","span":{"begin":123,"end":496},"obj":"Sentence"},{"id":"T3","span":{"begin":497,"end":660},"obj":"Sentence"},{"id":"T4","span":{"begin":661,"end":804},"obj":"Sentence"},{"id":"T5","span":{"begin":805,"end":936},"obj":"Sentence"},{"id":"T6","span":{"begin":937,"end":1036},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":161,"end":167},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":488,"end":495},"obj":"Glycan"},{"id":"T2","span":{"begin":601,"end":608},"obj":"Glycan"},{"id":"T3","span":{"begin":710,"end":716},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A4","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A5","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A6","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":488,"end":495},"obj":"Glycan"},{"id":"T2","span":{"begin":601,"end":608},"obj":"Glycan"},{"id":"T3","span":{"begin":710,"end":716},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A4","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A5","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A6","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":122},"obj":"Sentence"},{"id":"T2","span":{"begin":123,"end":496},"obj":"Sentence"},{"id":"T3","span":{"begin":497,"end":660},"obj":"Sentence"},{"id":"T4","span":{"begin":661,"end":804},"obj":"Sentence"},{"id":"T5","span":{"begin":805,"end":936},"obj":"Sentence"},{"id":"T6","span":{"begin":937,"end":1036},"obj":"Sentence"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":54,"end":76},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":123,"end":145},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"487"},{"id":"A2","pred":"db_id","subj":"T2","obj":"487"}],"text":"Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: a methodological comparison.\nNeisseria meningitidis trisaccharide [GlcNAc[(1--\u003e3)Galbeta(1--\u003e4)Glc-R], tetrasaccharide [Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e 3)Galbeta(1--\u003e4)Glc-R], and a pentasaccharide [Neu5Acalpha(2--\u003e3)Galbeta(1--\u003e4)GlcNAcbeta(1--\u003e3)G albeta(1--\u003e4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well as high yield (\u003e 90% from Gal(1--\u003e4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides."}