| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane. |
| T2 |
89-290 |
Sentence |
denotes |
B cell linker protein (BLNK) and Src homology 2 domain-containing leukocyte protein of 76 kD (SLP-76) are adaptor proteins required for B cell receptor (BCR) and T cell receptor function, respectively. |
| T3 |
291-392 |
Sentence |
denotes |
Here, we show that expression of SLP-76 cannot reconstitute BCR function in Zap-70(+)BLNK(-) B cells. |
| T4 |
393-541 |
Sentence |
denotes |
This could be attributable to inability of SLP-76 to be recruited into glycolipid-enriched microdomains (GEMs) after antigen receptor cross-linking. |
| T5 |
542-898 |
Sentence |
denotes |
Supporting this idea, the BCR function was restored when a membrane-associated SLP-76 chimera was enforcedly localized to GEMs. Moreover, we demonstrate that addition of both linker for activation of T cells (LAT) and Grb2-related adaptor downstream of Shc (Gads) to SLP-76 allow SLP-76 to be recruited into GEMs, whereby the BCR function is reconstituted. |
| T6 |
899-967 |
Sentence |
denotes |
The Gads function was able to be replaced by overexpression of Grb2. |
| T7 |
968-1235 |
Sentence |
denotes |
In contrast to SLP-76, BLNK did not require Grb2 families for its recruitment to GEMs. Hence, these data suggest a functional overlap between BLNK and SLP-76, while emphasizing the difference in requirement for additional adaptor molecules in their targeting to GEMs. |
