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PubMed:10993892 JSONTXT

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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 136-148 Cell denotes cancer cells http://purl.obolibrary.org/obo/CL:0001064

sentences

Id Subject Object Predicate Lexical cue
T1 0-149 Sentence denotes Erk1/2-dependent phosphorylation of Galpha-interacting protein stimulates its GTPase accelerating activity and autophagy in human colon cancer cells.
T2 150-323 Sentence denotes Galpha-interacting protein (GAIP) is a regulator of G protein signaling (RGS) that accelerates the rate of GTP hydrolysis by the alpha-subunit of the trimeric G(i3) protein.
T3 324-450 Sentence denotes Both proteins are part of a signaling pathway that controls lysosomal-autophagic catabolism in human colon cancer HT-29 cells.
T4 451-656 Sentence denotes Here we show that GAIP is phosphorylated by an extracellular signal-regulated (Erk1/2) MAP kinase-dependent pathway sensitive to amino acids, MEK1/2 (PD098059), and protein kinase C (GF109203X) inhibitors.
T5 657-992 Sentence denotes An in vitro phosphorylation assay demonstrates that Erk2-dependent phosphorylation of GAIP stimulates its GTPase-activating protein activity toward the Galpha(i3) protein (k = 0.187 +/- 0.001 s(-)(1), EC(50) = 1.12 +/- 0.10 microm) when compared with unphosphorylated recombinant GAIP (k = 0.145 +/- 0.003 s(-)(1), EC(50) = 3.16 +/- 0.
T6 993-1100 Sentence denotes 12 microm) or to GAIP phosphorylated by other Ser/Thr protein kinases (protein kinase C, casein kinase II).
T7 1101-1289 Sentence denotes This stimulation and the phosphorylation of GAIP by Erk2 were abrogated when serine at position 151 in the RGS domain was substituted by an alanine residue using site-directed mutagenesis.
T8 1290-1444 Sentence denotes Furthermore, the lysosomal-autophagic pathway was not stimulated in S151A-GAIP mutant-expressing cells when compared with wild-type GAIP-expressing cells.
T9 1445-1561 Sentence denotes These results demonstrate that the GTPase-activating protein activity of GAIP is stimulated by Erk2 phosphorylation.
T10 1562-1697 Sentence denotes They also suggested that Erk1/2 and GAIP are engaged in the signaling control of a major catabolic pathway in intestinal derived cells.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 130-135 http://purl.obolibrary.org/obo/MAT_0000526 denotes colon
T2 425-430 http://purl.obolibrary.org/obo/MAT_0000526 denotes colon
T3 1672-1682 http://purl.obolibrary.org/obo/MAT_0000043 denotes intestinal

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 425-437 HP_0003003 denotes colon cancer
T2 425-437 HP_0100273 denotes colon cancer
T3 431-437 HP_0002664 denotes cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
10993892-0#0#4#gene5595 0-4 gene5595 denotes Erk1
10993892-0#130#142#diseaseC0007102 130-142 diseaseC0007102 denotes colon cancer
10993892-0#130#142#diseaseC0699790 130-142 diseaseC0699790 denotes colon cancer
0#4#gene5595130#142#diseaseC0007102 10993892-0#0#4#gene5595 10993892-0#130#142#diseaseC0007102 associated_with Erk1,colon cancer
0#4#gene5595130#142#diseaseC0699790 10993892-0#0#4#gene5595 10993892-0#130#142#diseaseC0699790 associated_with Erk1,colon cancer

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 130-135 Body_part denotes colon http://purl.obolibrary.org/obo/MAT_0000526
T2 425-430 Body_part denotes colon http://purl.obolibrary.org/obo/MAT_0000526
T3 1672-1682 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 130-142 Phenotype denotes colon cancer HP:0003003
T2 425-437 Phenotype denotes colon cancer HP:0003003

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 130-142 Disease denotes colon cancer http://purl.obolibrary.org/obo/MONDO_0021063
T2 425-437 Disease denotes colon cancer http://purl.obolibrary.org/obo/MONDO_0021063

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 124-129 OrganismTaxon denotes human 9606
T2 419-424 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 130-135 Body_part denotes colon http://purl.obolibrary.org/obo/UBERON_0001155
T2 425-430 Body_part denotes colon http://purl.obolibrary.org/obo/UBERON_0001155
T3 498-511 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576