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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 36-42 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T2 116-122 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T3 240-251 https://glytoucan.org/Structures/Glycans/G25326UC denotes xyloglucans
T4 357-363 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T5 399-406 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T6 426-433 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T7 551-558 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T8 603-609 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T9 636-642 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T10 988-995 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T11 1010-1016 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T12 1095-1101 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T13 1405-1411 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T14 1564-1571 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T15 1838-1844 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 36-42 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T2 116-122 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T3 240-251 Glycan_Motif denotes xyloglucans https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC
T4 357-363 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T5 399-406 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T6 426-433 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T7 551-558 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T8 603-609 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T9 636-642 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T10 988-995 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T11 1010-1016 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T12 1095-1101 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T13 1405-1411 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T14 1564-1571 Glycan_Motif denotes mannose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ
T15 1838-1844 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 36-42 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T2 116-122 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T3 240-251 https://glytoucan.org/Structures/Glycans/G25326UC denotes xyloglucans
T4 357-363 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T5 399-406 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T6 426-433 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T7 551-558 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T8 603-609 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T9 636-642 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T10 988-995 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T11 1010-1016 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T12 1095-1101 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T13 1405-1411 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T14 1564-1571 https://glytoucan.org/Structures/Glycans/G70323CJ denotes mannose
T15 1838-1844 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 378-389 HP_0001944 denotes dehydration

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T2 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T3 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T4 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T7 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T8 698-714 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli
_T9 698-718 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC720 denotes Escherichia coli K12
_T10 698-718 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli K12

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 36-42 FMAID:82790 denotes fucose
_T2 36-42 FMAID:196784 denotes fucose
_T3 116-122 FMAID:196784 denotes fucose
_T4 116-122 FMAID:82790 denotes fucose
_T5 169-184 FMAID:82782 denotes glycoconjugates
_T6 169-184 FMAID:196776 denotes glycoconjugates
_T7 204-223 FMAID:82746 denotes lipopolysaccharides
_T8 204-223 FMAID:82785 denotes lipopolysaccharides
_T9 204-223 FMAID:196779 denotes lipopolysaccharides
_T10 204-223 FMAID:196735 denotes lipopolysaccharides
_T11 226-232 FMAID:214748 denotes plants
_T12 272-283 FMAID:196773 denotes glycolipids
_T13 272-283 FMAID:82780 denotes glycolipids
_T14 285-298 FMAID:167256 denotes glycoproteins
_T15 285-298 FMAID:62925 denotes glycoproteins
_T16 304-320 FMAID:196731 denotes oligosaccharides
_T17 304-320 FMAID:82742 denotes oligosaccharides
_T18 357-363 FMAID:196784 denotes fucose
_T19 357-363 FMAID:82790 denotes fucose
_T20 399-406 FMAID:82801 denotes mannose
_T21 399-406 FMAID:196796 denotes mannose
_T22 426-433 FMAID:82801 denotes mannose
_T23 426-433 FMAID:196796 denotes mannose
_T24 551-558 FMAID:82801 denotes mannose
_T25 551-558 FMAID:196796 denotes mannose
_T26 603-609 FMAID:82790 denotes fucose
_T27 603-609 FMAID:196784 denotes fucose
_T28 636-642 FMAID:82790 denotes fucose
_T29 636-642 FMAID:196784 denotes fucose
_T30 662-667 FMAID:198663 denotes genes
_T31 770-772 FMAID:63509 denotes T7
_T32 770-772 FMAID:167737 denotes T7
_T33 878-886 FMAID:67257 denotes proteins
_T34 878-886 FMAID:165447 denotes proteins
_T35 988-995 FMAID:82801 denotes mannose
_T36 988-995 FMAID:196796 denotes mannose
_T37 1010-1016 FMAID:82790 denotes fucose
_T38 1010-1016 FMAID:196784 denotes fucose
_T39 1095-1101 FMAID:196784 denotes fucose
_T40 1095-1101 FMAID:82790 denotes fucose
_T41 1216-1224 FMAID:165447 denotes proteins
_T42 1216-1224 FMAID:67257 denotes proteins
_T43 1405-1411 FMAID:82790 denotes fucose
_T44 1405-1411 FMAID:196784 denotes fucose
_T45 1495-1501 FMAID:165145 denotes microM
_T46 1514-1524 FMAID:166112 denotes mg protein
_T47 1517-1524 FMAID:165447 denotes protein
_T48 1517-1524 FMAID:67257 denotes protein
_T49 1564-1571 FMAID:196796 denotes mannose
_T50 1564-1571 FMAID:82801 denotes mannose
_T51 1582-1588 FMAID:165145 denotes microM
_T52 1601-1611 FMAID:166112 denotes mg protein
_T53 1604-1611 FMAID:165447 denotes protein
_T54 1604-1611 FMAID:67257 denotes protein
_T55 1767-1770 FMAID:182506 denotes Ca2
_T56 1767-1770 FMAID:272658 denotes Ca2
_T57 1767-1770 FMAID:272430 denotes Ca2
_T58 1838-1844 FMAID:82790 denotes fucose
_T59 1838-1844 FMAID:196784 denotes fucose

uniprot-human

Id Subject Object Predicate Lexical cue
T1 715-718 http://www.uniprot.org/uniprot/Q99456 denotes K12
T2 715-718 http://www.uniprot.org/uniprot/Q8WVN6 denotes K12
T3 986-1002 http://www.uniprot.org/uniprot/O60547 denotes D-mannose to GDP

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 668-671 http://www.uniprot.org/uniprot/Q8K0C9 denotes gmd
T2 715-718 http://www.uniprot.org/uniprot/Q64291 denotes K12
T3 715-718 http://www.uniprot.org/uniprot/Q9JL59 denotes K12
T4 1767-1770 http://www.uniprot.org/uniprot/P00920 denotes Ca2

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 29-33 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T2 29-33 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T3 71-86 http://purl.bioontology.org/ontology/NCBITAXON/547 denotes enterobacterial
T4 71-86 http://purl.bioontology.org/ontology/NCBITAXON/543 denotes enterobacterial
T5 71-86 http://purl.bioontology.org/ontology/NCBITAXON/539813 denotes enterobacterial
T6 71-86 http://purl.bioontology.org/ontology/NCBITAXON/885040 denotes enterobacterial
T7 188-196 http://purl.bioontology.org/ontology/NCBITAXON/2 denotes bacteria
T8 188-196 http://purl.bioontology.org/ontology/NCBITAXON/629395 denotes bacteria
T9 596-600 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T10 596-600 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T11 629-633 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T12 629-633 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T13 698-709 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T14 1003-1007 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T15 1003-1007 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T16 1088-1092 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T17 1088-1092 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T18 1398-1402 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T19 1398-1402 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T20 1741-1749 http://purl.bioontology.org/ontology/NCBITAXON/433098 denotes divalent
T21 1831-1835 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T22 1831-1835 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta

GO-BP

Id Subject Object Predicate Lexical cue
T1 12-21 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T2 12-28 http://purl.obolibrary.org/obo/GO_0046711 denotes synthesis of GDP
T3 451-454 http://purl.obolibrary.org/obo/GO_0008446 denotes Gmd
T4 924-927 http://purl.obolibrary.org/obo/GO_0008446 denotes Gmd
T5 1203-1206 http://purl.obolibrary.org/obo/GO_0008446 denotes Gmd
T6 1272-1275 http://purl.obolibrary.org/obo/GO_0008446 denotes Gmd
T7 668-671 http://purl.obolibrary.org/obo/GO_0008446 denotes gmd
T8 1071-1080 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T9 1071-1087 http://purl.obolibrary.org/obo/GO_0046711 denotes formation of GDP

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 226-232 http://edamontology.org/topic_0780 denotes plants
T2 340-347 http://edamontology.org/topic_0602 denotes pathway
T3 773-781 http://edamontology.org/topic_1312 denotes promoter
T4 773-781 http://edamontology.org/topic_0749 denotes promoter
T5 773-781 http://edamontology.org/topic_0111 denotes promoter
T6 854-862 http://edamontology.org/topic_0749 denotes terminal
T7 878-886 http://edamontology.org/topic_0078 denotes proteins
T8 1154-1157 http://edamontology.org/topic_0593 denotes NMR
T9 1216-1224 http://edamontology.org/topic_0078 denotes proteins
T10 1517-1524 http://edamontology.org/topic_0078 denotes protein
T11 1604-1611 http://edamontology.org/topic_0078 denotes protein

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 340-347 http://edamontology.org/data_2600 denotes pathway
T2 878-886 http://edamontology.org/data_1467 denotes proteins
T3 878-886 http://edamontology.org/format_1208 denotes proteins
T4 966-976 http://edamontology.org/operation_3434 denotes conversion
T5 1071-1080 http://edamontology.org/format_1915 denotes formation
T6 1071-1080 http://edamontology.org/operation_0335 denotes formation
T7 1216-1224 http://edamontology.org/data_1467 denotes proteins
T8 1216-1224 http://edamontology.org/format_1208 denotes proteins
T9 1455-1457 http://edamontology.org/data_0910 denotes Km
T10 1462-1466 http://edamontology.org/data_0909 denotes Vmax
T11 1517-1524 http://edamontology.org/format_1208 denotes protein
T12 1517-1524 http://edamontology.org/data_1467 denotes protein
T13 1604-1611 http://edamontology.org/format_1208 denotes protein
T14 1604-1611 http://edamontology.org/data_1467 denotes protein
T15 1655-1657 http://edamontology.org/format_1997 denotes pH

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
TextSentencer_T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
TextSentencer_T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
TextSentencer_T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
TextSentencer_T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
TextSentencer_T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
TextSentencer_T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
TextSentencer_T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
TextSentencer_T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
TextSentencer_T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
TextSentencer_T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.
T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.
T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 240-251 http://www.glycoepitope.jp/epitopes/EP0511 denotes xyloglucans

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 240-251 Glycan denotes xyloglucans https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 378-389 Phenotype denotes dehydration HP:0001944

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 378-389 Phenotype denotes dehydration HP:0001944

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-95 Sentence denotes Preparative synthesis of GDP-beta-L-fucose by recombinant enzymes from enterobacterial sources.
T2 96-322 Sentence denotes The 6-deoxyhexose L-fucose is an important and characteristic element in glycoconjugates of bacteria (e.g., lipopolysaccharides), plants (e.g., xyloglucans) and animals (e.g., glycolipids, glycoproteins, and oligosaccharides).
T3 323-643 Sentence denotes The biosynthetic pathway of GDP-L-fucose starts with a dehydration of GDP-D-mannose catalyzed by GDP-D-mannose 4,6-dehydratase (Gmd) creating GDP-4-keto-6-deoxymannose which is subsequently converted by the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase (WcaG; GDP-beta-L-fucose synthetase) to GDP-beta-L-fucose.
T4 644-901 Sentence denotes Both biosynthetic genes gmd and wcaG were cloned from Escherichia coli K12 and the enzymes overexpressed under control of the T7 promoter in the expression vectors pET11a and pET16b, yielding both native and N-terminal His-tag fusion proteins, respectively.
T5 902-951 Sentence denotes The activities of the Gmd and WcaG were analyzed.
T6 952-1066 Sentence denotes The enzymatic conversion from GDP-D-mannose to GDP-beta-L-fucose was optimized and the final product was purified.
T7 1067-1167 Sentence denotes The formation of GDP-beta-L-fucose by the recombinant enzymes was verified by HPLC and NMR analyses.
T8 1168-1259 Sentence denotes The His-tag fusion variants of the Gmd and WcaG proteins were purified to near homogeneity.
T9 1260-1423 Sentence denotes The His-tag Gmd recombinant enzyme was inactive, whereas His-tag WcaG showed very similar enzymatic properties relative to the native GDP-beta-L-fucose synthetase.
T10 1424-1772 Sentence denotes With the purified His-tag WcaG Km and Vmax values, respectively, of 40 microM and 23 nkat/mg protein for the substrate GDP-4-keto-6-deoxy-D-mannose and of 21 microM and 10 nkat/mg protein for the cosubstrate NADPH were obtained; a pH optimum of 7.5 was determined and the enzyme was stimulated to equal extend by the divalent cations Mg2+ and Ca2+.
T11 1773-1845 Sentence denotes The Gmd enzyme showed a strong feedback inhibition by GDP-beta-L-fucose.

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 188-196 Organism denotes bacteria 2|629395
T3 698-714 Organism denotes Escherichia coli 562

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 188-196 OrganismTaxon denotes bacteria 2|629395
T3 698-714 OrganismTaxon denotes Escherichia coli 562