PubMed:10972871 JSON TXT

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OryzaGP_2021

Oryza-OGER

OryzaGP_2022

{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":124,"end":125},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T2","span":{"begin":341,"end":342},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T3","span":{"begin":409,"end":410},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T4","span":{"begin":418,"end":419},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T5","span":{"begin":825,"end":826},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T6","span":{"begin":995,"end":996},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T7","span":{"begin":1108,"end":1109},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T8","span":{"begin":1273,"end":1274},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T9","span":{"begin":1449,"end":1450},"obj":"http://identifiers.org/oryzabase.gene/11216"}],"text":"dull: rice mutants with tissue-specific effects on the splicing of the waxy pre-mRNA.\nIn the endosperm of japonica rice, du-1 and du-2 mutations cause the reduction of amylose contents. It was previously shown that the Wx(b) allele of rice, which is predominantly distributed in japonica rice, has a mutation in the 5' splice site of intron 1 resulting in the creation of two weak 5' splice sites within exon 1. In du-1 and du-2 mutants, spliced Wx(b) transcripts were highly reduced, whereas the processing of transcripts derived from three other genes highly expressed in endosperm was not apparently influenced. Results of competitive RT-PCR analysis indicate that transcripts spliced at the two newly created 5' splice sites were equally affected in these two mutants. Genetic and molecular analyses of the effects of du-1 and du-2 on Wx(a) pre-mRNA with normal splice sites indicate that these two mutations do not affect the processing of Wx(a) pre-mRNA after splicing, suggesting that du-1 and du-2 are mutations of genes required for the efficient splicing of mutated Wx(b) pre-mRNA. Furthermore, du-1 and du-2 showed differential effects in endosperm and pollen. Although both mutations caused similar effects on the splicing of Wx(a) transcripts in endosperm, du-1 caused higher reduction of Wx(b) mRNA in pollen than in endosperm, while du-2 had a lesser effect in pollen than in endosperm. Based on these results, we propose that the du-1 and du-2 loci of rice encode tissue-specifically regulated splicing factors that are involved in alternative splicing of pre-mRNA in rice."}

OryzaGP_2021_v2

OryzaGP_2021_FLAIR

OryzaGP

{"project":"OryzaGP","denotations":[{"id":"T1","span":{"begin":71,"end":75},"obj":"gene"}],"text":"dull: rice mutants with tissue-specific effects on the splicing of the waxy pre-mRNA.\nIn the endosperm of japonica rice, du-1 and du-2 mutations cause the reduction of amylose contents. It was previously shown that the Wx(b) allele of rice, which is predominantly distributed in japonica rice, has a mutation in the 5' splice site of intron 1 resulting in the creation of two weak 5' splice sites within exon 1. In du-1 and du-2 mutants, spliced Wx(b) transcripts were highly reduced, whereas the processing of transcripts derived from three other genes highly expressed in endosperm was not apparently influenced. Results of competitive RT-PCR analysis indicate that transcripts spliced at the two newly created 5' splice sites were equally affected in these two mutants. Genetic and molecular analyses of the effects of du-1 and du-2 on Wx(a) pre-mRNA with normal splice sites indicate that these two mutations do not affect the processing of Wx(a) pre-mRNA after splicing, suggesting that du-1 and du-2 are mutations of genes required for the efficient splicing of mutated Wx(b) pre-mRNA. Furthermore, du-1 and du-2 showed differential effects in endosperm and pollen. Although both mutations caused similar effects on the splicing of Wx(a) transcripts in endosperm, du-1 caused higher reduction of Wx(b) mRNA in pollen than in endosperm, while du-2 had a lesser effect in pollen than in endosperm. Based on these results, we propose that the du-1 and du-2 loci of rice encode tissue-specifically regulated splicing factors that are involved in alternative splicing of pre-mRNA in rice."}