PubMed:10944123 JSONTXT

{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":70,"end":75},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T2","span":{"begin":96,"end":100},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T3","span":{"begin":486,"end":491},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T4","span":{"begin":493,"end":498},"obj":"https://acgg.asia/db/ggdb/info/gg216"},{"id":"T5","span":{"begin":500,"end":505},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"T6","span":{"begin":510,"end":514},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T7","span":{"begin":580,"end":585},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T8","span":{"begin":596,"end":600},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T9","span":{"begin":679,"end":684},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T10","span":{"begin":759,"end":764},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T11","span":{"begin":769,"end":773},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T12","span":{"begin":775,"end":780},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T13","span":{"begin":827,"end":832},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T14","span":{"begin":857,"end":861},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T15","span":{"begin":982,"end":987},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T16","span":{"begin":989,"end":994},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"T17","span":{"begin":999,"end":1003},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T18","span":{"begin":1034,"end":1038},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T19","span":{"begin":1093,"end":1097},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T20","span":{"begin":1195,"end":1199},"obj":"https://acgg.asia/db/ggdb/info/gg203"}],"text":"Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.\nGlycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP- N-acetylglucosamine to phosphatidylinositol. GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI."}

{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":70,"end":75},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T2","span":{"begin":96,"end":100},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T3","span":{"begin":486,"end":491},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T4","span":{"begin":493,"end":498},"obj":"https://acgg.asia/db/ggdb/info/gg216"},{"id":"T5","span":{"begin":500,"end":505},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"T6","span":{"begin":510,"end":514},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T7","span":{"begin":580,"end":585},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T8","span":{"begin":596,"end":600},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T9","span":{"begin":679,"end":684},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T10","span":{"begin":759,"end":764},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T11","span":{"begin":769,"end":773},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T12","span":{"begin":775,"end":780},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T13","span":{"begin":827,"end":832},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"T14","span":{"begin":857,"end":861},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T15","span":{"begin":982,"end":987},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"T16","span":{"begin":989,"end":994},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"T17","span":{"begin":999,"end":1003},"obj":"https://acgg.asia/db/ggdb/info/gg213"},{"id":"T18","span":{"begin":1034,"end":1038},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T19","span":{"begin":1093,"end":1097},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"T20","span":{"begin":1195,"end":1199},"obj":"https://acgg.asia/db/ggdb/info/gg203"}],"text":"Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.\nGlycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP- N-acetylglucosamine to phosphatidylinositol. GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI."}

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":0,"end":101},"obj":"Sentence"},{"id":"T2","span":{"begin":102,"end":222},"obj":"Sentence"},{"id":"T3","span":{"begin":223,"end":396},"obj":"Sentence"},{"id":"T4","span":{"begin":397,"end":515},"obj":"Sentence"},{"id":"T5","span":{"begin":516,"end":678},"obj":"Sentence"},{"id":"T6","span":{"begin":679,"end":774},"obj":"Sentence"},{"id":"T7","span":{"begin":775,"end":856},"obj":"Sentence"},{"id":"T8","span":{"begin":857,"end":1004},"obj":"Sentence"},{"id":"T9","span":{"begin":1005,"end":1200},"obj":"Sentence"},{"id":"T10","span":{"begin":1201,"end":1392},"obj":"Sentence"}],"text":"Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.\nGlycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP- N-acetylglucosamine to phosphatidylinositol. GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI."}

{"project":"glycogenes","denotations":[{"id":"PD-GlycoGenes20190927-B_T1","span":{"begin":70,"end":75},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"PD-GlycoGenes20190927-B_T2","span":{"begin":96,"end":100},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"PD-GlycoGenes20190927-B_T3","span":{"begin":486,"end":491},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"PD-GlycoGenes20190927-B_T4","span":{"begin":493,"end":498},"obj":"https://acgg.asia/db/ggdb/info/gg216"},{"id":"PD-GlycoGenes20190927-B_T5","span":{"begin":500,"end":505},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"PD-GlycoGenes20190927-B_T6","span":{"begin":580,"end":585},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"PD-GlycoGenes20190927-B_T7","span":{"begin":596,"end":600},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"PD-GlycoGenes20190927-B_T8","span":{"begin":679,"end":684},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"PD-GlycoGenes20190927-B_T9","span":{"begin":759,"end":764},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"PD-GlycoGenes20190927-B_T10","span":{"begin":775,"end":780},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"PD-GlycoGenes20190927-B_T11","span":{"begin":827,"end":832},"obj":"https://acgg.asia/db/ggdb/info/gg217"},{"id":"PD-GlycoGenes20190927-B_T12","span":{"begin":857,"end":861},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"PD-GlycoGenes20190927-B_T13","span":{"begin":982,"end":987},"obj":"https://acgg.asia/db/ggdb/info/gg215"},{"id":"PD-GlycoGenes20190927-B_T14","span":{"begin":989,"end":994},"obj":"https://acgg.asia/db/ggdb/info/gg214"},{"id":"PD-GlycoGenes20190927-B_T15","span":{"begin":1034,"end":1038},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"PD-GlycoGenes20190927-B_T16","span":{"begin":1093,"end":1097},"obj":"https://acgg.asia/db/ggdb/info/gg203"},{"id":"PD-GlycoGenes20190927-B_T17","span":{"begin":1195,"end":1199},"obj":"https://acgg.asia/db/ggdb/info/gg203"}],"text":"Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2.\nGlycosylphosphatidylinositols (GPIs) are attached to the C-termini of many proteins, thereby acting as membrane anchors. Biosynthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), which transfers N-acetylglucosamine from UDP- N-acetylglucosamine to phosphatidylinositol. GPI-GnT is a uniquely complex glycosyltransferase, consisting of at least four proteins, PIG-A, PIG-H, PIG-C and GPI1. Here, we report that GPI-GnT requires another component, termed PIG-P, and that DPM2, which regulates dolichol-phosphate-mannose synthase, also regulates GPI-GnT. PIG-P, a 134-amino acid protein having two hydrophobic domains, associates with PIG-A and GPI1. PIG-P is essential for GPI-GnT since a cell lacking PIG-P is GPI-anchor negative. DPM2, but not two other components of dolichol-phosphate-mannose synthase, associates with GPI-GnT through interactions with PIG-A, PIG-C and GPI1. Lec15 cell, a null mutant of DPM2, synthesizes early GPI intermediates, indicating that DPM2 is not essential for GPI-GnT; however, the enzyme activity is enhanced 3-fold in the presence of DPM2. These results reveal new essential and regulatory components of GPI-GnT and imply co-regulation of GPI-GnT and the dolichol-phosphate-mannose synthase that generates a mannosyl donor for GPI."}