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PubMed:10902765 JSONTXT

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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 197-730 OBJECTIVE denotes Osteopontin (OPN) is an extracellular matrix protein that has been implicated in the interactions between tumor cells and host matrix, including those involved in invasion and spread of tumor cells. Because joint destruction in rheumatoid arthritis (RA) is mediated by the invasive growth of synovial tissue through its attachment to cartilage, we examined the expression of OPN in the synovia of patients with RA and the effect of OPN on the production of collagenase 1 in rheumatoid synovial fibroblasts and articular chondrocytes.
T2 740-1162 METHODS denotes The expression of OPN messenger RNA (mRNA) and protein in synovia from 10 RA patients was examined by in situ hybridization and immunohistochemistry. Synovial fibroblasts from RA patients and articular chondrocytes from patients without joint disease were cultured in the presence of various concentrations of OPN, and levels of collagenase 1 in the culture supernatants were measured by enzyme-linked immunosorbent assay.
T3 1172-2108 RESULTS denotes The expression of OPN mRNA and protein was observed in 9 of 10 specimens obtained from patients with RA. OPN was expressed in the synovial lining and sublining layer and at the interface of cartilage and invading synovium. Double labeling revealed that the majority of OPN-expressing cells were positive for the fibroblast-specific enzyme prolyl 4-hydroxylase and negative for the macrophage marker CD68, while only a few, single OPN-expressing cells were positive for CD68 at sites of synovial invasion into cartilage. OPN staining was not observed in lymphocytic infiltrates or leukocyte common antigen (CD45)-positive cells. Three of 3 cultures of human articular chondrocytes secreted detectable basal amounts of collagenase, with a dose-dependent increase upon OPN stimulation, while synovial fibroblast cultures produced much lower levels of collagenase, with only 2 of 4 fibroblast cultures responding in a dose-dependent manner.
T4 2121-2429 CONCLUSIONS denotes These findings suggest that OPN produced by synovial fibroblasts in the synovial lining layer and at sites of cartilage invasion not only mediates attachment of these cells to cartilage, but also contributes to matrix degradation in RA by stimulating the secretion of collagenase 1 in articular chondrocytes.

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 303-308 HP_0002664 denotes tumor
T2 383-388 HP_0002664 denotes tumor
T3 425-445 HP_0001370 denotes rheumatoid arthritis
T4 436-445 HP_0001369 denotes arthritis

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
10902765-0#14#25#gene6696 14-25 gene6696 denotes osteopontin
10902765-0#88#99#gene6696 88-99 gene6696 denotes osteopontin
10902765-0#55#75#diseaseC0003873 55-75 diseaseC0003873 denotes rheumatoid arthritis
10902765-4#160#163#gene6696 1050-1053 gene6696 denotes OPN
10902765-4#87#100#diseaseC0022408 977-990 diseaseC0022408 denotes joint disease
14#25#gene669655#75#diseaseC0003873 10902765-0#14#25#gene6696 10902765-0#55#75#diseaseC0003873 associated_with osteopontin,rheumatoid arthritis
88#99#gene669655#75#diseaseC0003873 10902765-0#88#99#gene6696 10902765-0#55#75#diseaseC0003873 associated_with osteopontin,rheumatoid arthritis
160#163#gene669687#100#diseaseC0022408 10902765-4#160#163#gene6696 10902765-4#87#100#diseaseC0022408 associated_with OPN,joint disease

DisGeNET

Id Subject Object Predicate Lexical cue
T0 572-575 gene:6696 denotes OPN
T1 425-445 disease:C0003873 denotes rheumatoid arthritis
T2 572-575 gene:6696 denotes OPN
T3 447-449 disease:C0003873 denotes RA
T4 572-575 gene:6696 denotes OPN
T5 608-610 disease:C0003873 denotes RA
T6 629-632 gene:6696 denotes OPN
T7 425-445 disease:C0003873 denotes rheumatoid arthritis
T8 629-632 gene:6696 denotes OPN
T9 447-449 disease:C0003873 denotes RA
T10 629-632 gene:6696 denotes OPN
T11 608-610 disease:C0003873 denotes RA
R1 T0 T1 associated_with OPN,rheumatoid arthritis
R2 T2 T3 associated_with OPN,RA
R3 T4 T5 associated_with OPN,RA
R4 T6 T7 associated_with OPN,rheumatoid arthritis
R5 T8 T9 associated_with OPN,RA
R6 T10 T11 associated_with OPN,RA